Determination of oligosaccharides by capillary zone electrophoresis

Arentoft, Anne Marie; Michaelsen, Søren; Sørensen, Hilmer

doi:10.1016/0021-9673(93)83273-u

Cited by 45 publications

(16 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A powerful alternative for HPAEC-MS can be CE [33][34][35][36][37]. CE evolved as a promising alternative in carbohydrate analysis with respect to fast and highly efficient separations [38].…”

Section: Introductionmentioning

confidence: 99%

CE‐MSⁿ of complex pectin‐derived oligomers

Coenen¹,

Kabel²,

Schols³

et al. 2008

Electrophoresis

View full text Add to dashboard Cite

As pectin molecules are too large and heterogeneous to analyze as a whole, the polymer is usually degraded to smaller oligomers, which are often analyzed by high-performance anion exchange chromatography (HPAEC). However, the high salt concentration necessary to elute pectin oligomers by HPAEC is incompatible with online mass detection. To overcome such a disadvantage, a CE-IT-MS system was set up to further elucidate the fine structure of charged oligosaccharides. An effective separation of differently substituted galacturonic acid containing oligomers was obtained by low-pH CE-LIF analysis. By adapting the buffer and capillary online MS detection was enabled. Moreover, with MS/MS it was possible to localize sugar residues' substitutions. With this combined CE-MS approach LIF electropherograms of xylogalacturonan and rhamnogalacturonan I digests could be annotated. The method was further exemplified by a complex oligomer mixture of acid hydrolyzed apple pectin, which was separated and characterized by CE-MSn. Oligomers present in low amounts could be localized by their corresponding m/z, as was demonstrated by selected mass range representation.

show abstract

Section: Introductionmentioning

confidence: 99%

CE‐MSⁿ of complex pectin‐derived oligomers

Coenen¹,

Kabel²,

Schols³

et al. 2008

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…Arentoft et al [12] used MEKC, with cholate as detergent, to separate immuno-complexes and, most recently, Steinmann et al [13] determined theophylline in serum using SDS as buffer additive. These authors did not evaluate the denaturing effect of the detergent, although previous work [14] has shown that SDS binding (even at submillimolar concentration) results in protein denaturation with potential loss of affinity.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the biotin‐binding protein actinavidin using affinity capillary electrophoresis

Okun

Bilitewski

1996

Electrophoresis

View full text Add to dashboard Cite

Affinity capillary electrophoresis (ACE) was applied to study the bioaffinity of ligand-receptor interaction between the microbial biotin-binding protein actinavidin and biotin. The ACE method is based on short time incubation of a mixture of actinavidin and increasing concentrations of biotinylated oligonucleotide (bio-ON), which was found to be an effective affinity ligand. Separation of intermediate loading forms of actinavidin from unbound ligand in the presence of micellar phase and by capillary zone electrophoresis enabled the quantitation of free bio-ON, permitting the evaluation of the biotin-binding capacity of actinavidin in absence and presence of sodium dodecyl sulfate (SDS). Although in the latter case actinavidin lost a part of its binding capacity (not more than 12%), it was still possible to develop an indirect, noncompetitive assay for the determination of actinavidin in culture liquid, utilizing the combination of micellar electrokinetic capillary chromatography (MEKC) and ACE. Due to the affinity interaction, actinavidin in the sample decreases the amount of bio-ON added, enabling quantitation of the protein. SDS, which is required in this assay to prevent protein adsorption to the capillary wall, greatly enhances the reproducibility and peak shape. Actinavidin levels determined are in agreement with those obtained by commonly used solid-phase analysis. The limit of detection was about 500 ng/mL. Thus the proposed method was found to be well suited for the evaluation of actinavidin affinity and monitoring of its levels in cultivation process.

show abstract

“…Raffinose family oligosaccharides (RFOs), also known as α-galactosides, are galactosylic derivatives of sucrose whose biosynthesis starts during seed development (Arentoft et al, 1993;Piotrowicz-Cieślak, 2005). Under natural conditions, an intense accumulation of RFOs occurs near the end of seed ripening (Blackman et al, 1992;Górecki et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

The effect of irrigation, soil cultivation system and nitrogen fertilizer on the vitality and content of selected sugars in Vicia faba seed

Kurasiak-Popowska

Szukała

Gulewicz³

2009

Span. j. agric. res.

View full text Add to dashboard Cite

In this study the influence of sprinkler irrigation, various soil cultivation systems (conventional, reduced tillage, zero tillage system) and the level (0, 30, 60, 90 kg N ha -1 ) of nitrogen (N) fertilization on the vitality and content of selected sugars in faba bean seeds (Vicia faba L.) of the cultivar Nadwiślański was examined. Sprinkler irrigation of faba bean improved seed energy and germination in all three years of the study (1999)(2000)(2001)) -on average germination energy by 8.8% and total germination by 3.2%-. Germination of faba bean seed under conventional tillage in the drier years was significantly higher than in the zero tillage system. In the wetter year, seed from both simplified systems produced seeds with higher germination than in traditional conventional tillage. Nitrogen (N) fertilizer affected germination energy, but had no effect on faba bean germination. Sprinkler irrigation and N fertilization had no effect on the content of the sugars studied in the faba bean seed. However, the stachyose content of faba bean seeds from conventional tilled plants was significantly higher than in seed of zero tilled plants (0.78 mg g -1 seed dm), and the galactose content of seed from zero tilled plants was significantly higher than in the other two cultivation systems -0.34 and 0.28 mg g -1 seed dm in seeds from conventional and reduced tillage system, respectively. Additional key words: agronomic treatment, faba bean seeds, RFOs sugars. ResumenEfectos del regadío de primavera, del manejo del suelo y de la fertilización nitrogenada sobre la vitalidad de las semillas y el contenido de azúcares en Vicia faba El objetivo de este estudio fue evaluar los efectos del riego por aspersión bajo diferentes sistemas de laboreo (tradicional, reducido o no laboreo) y con diferentes dosis (0, 30, 60 y 90 kg N ha -1 ) de fertilización de nitrógeno, sobre la vitalidad y el contenido de azúcares en semillas de haba cv. Nadwislanski. Se observó que en los tres años del estudio (1999-2001) el riego mejoró la energía y capacidad de germinación de las semillas -de media la energía de la germinación un 8,8% y la germinación total un 3,2%-. La capacidad de germinación de las semillas de habas bajo laboreo tradicional en los años más secos fue significativamente mayor que sin laboreo. En el año más húmedo, las habas bajo laboreo reducido produjeron semillas con mayor capacidad de germinación que bajo laboreo tradicional. La fertilización nitrogenada produjo diferencias en la energía de la germinación pero no tuvo efecto sobre la capacidad de germinación. El riego y la fertilización de N no tuvieron efecto sobre el contenido de los azúcares estudiados en las semillas. Sin embargo, bajo laboreo tradicional, el contenido de estaquiosa en semillas fue significativamente más alto que sin laboreo (0,78 mg g -1 semillas dm), y el contenido de galactosa en semillas sin laboreo fue significativamente más alto que en los otros dos sistemas de laboreo (0,34 y 0,28 mg g -1 semillas dm en semillas cultivadas bajo laboreo tradi...

show abstract

Determination of oligosaccharides by capillary zone electrophoresis

Cited by 45 publications

References 12 publications

CE‐MSⁿ of complex pectin‐derived oligomers

CE‐MSⁿ of complex pectin‐derived oligomers

Analysis of the biotin‐binding protein actinavidin using affinity capillary electrophoresis

The effect of irrigation, soil cultivation system and nitrogen fertilizer on the vitality and content of selected sugars in Vicia faba seed

Contact Info

Product

Resources

About

Determination of oligosaccharides by capillary zone electrophoresis

Cited by 45 publications

References 12 publications

CE‐MSn of complex pectin‐derived oligomers

CE‐MSn of complex pectin‐derived oligomers

Analysis of the biotin‐binding protein actinavidin using affinity capillary electrophoresis

The effect of irrigation, soil cultivation system and nitrogen fertilizer on the vitality and content of selected sugars in Vicia faba seed

Contact Info

Product

Resources

About

CE‐MSⁿ of complex pectin‐derived oligomers

CE‐MSⁿ of complex pectin‐derived oligomers