Determination of oregonin in Alnus plants and biological samples by capillary electrophoresis

Kuo, Ching‐Hua; Lee, Chia-Wen; Lai, Yi-Chun; Lee, Shoei‐Sheng

doi:10.1016/j.jpba.2007.12.012

Cited by 11 publications

(7 citation statements)

References 14 publications

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“…A possible connection to the observed effects might be the diarylheptanoid derivate oregonin, mainly found in Alnus species, which was reported to display anti-inflammatory and anti-oxidative activities (Kuo et al, 2008). …”

Section: Resultsmentioning

confidence: 99%

Ethnopharmacological in vitro studies on Austria's folk medicine—An unexplored lore in vitro anti-inflammatory activities of 71 Austrian traditional herbal drugs

Vogl

Picker

Mihály-Bison

et al. 2013

Journal of Ethnopharmacology

225

130

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Ethnopharmacological relevanceIn Austria, like in most Western countries, knowledge about traditional medicinal plants is becoming scarce. Searching the literature concerning Austria's ethnomedicine reveals its scant scientific exploration.Aiming to substantiate the potential of medicinal plants traditionally used in Austria, 63 plant species or genera with claimed anti-inflammatory properties listed in the VOLKSMED database were assessed for their in vitro anti-inflammatory activity.Material and methods71 herbal drugs from 63 plant species or genera were extracted using solvents of varying polarities and subsequently depleted from the bulk constituents, chlorophylls and tannins to avoid possible interferences with the assays. The obtained 257 extracts were assessed for their in vitro anti-inflammatory activity. The expression of the inflammatory mediators E-selectin and interleukin-8 (IL-8), induced by the inflammatory stimuli tumor necrosis factor alpha (TNF-α) and the bacterial product lipopolysaccharide (LPS) was measured in endothelial cells. The potential of the extracts to activate the nuclear factors PPARα and PPARγ and to inhibit TNF-α-induced activation of the nuclear factor-kappa B (NF-κB) in HEK293 cells was determined by luciferase reporter gene assays.ResultsIn total, extracts from 67 of the 71 assessed herbal drugs revealed anti-inflammatory activity in the applied in vitro test systems. Thereby, 30 could downregulate E-selectin or IL-8 gene expression, 28 were strong activators of PPARα or PPARγ (inducing activation of more than 2-fold at a concentration of 10 µg/mL) and 21 evoked a strong inhibition of NF-κB (inhibition of more than 80% at 10 µg/mL).ConclusionOur research supports the efficacy of herbal drugs reported in Austrian folk medicine used for ailments associated with inflammatory processes. Hence, an ethnopharmacological screening approach is a useful tool for the discovery of new drug leads.

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Section: Resultsmentioning

confidence: 99%

Ethnopharmacological in vitro studies on Austria's folk medicine—An unexplored lore in vitro anti-inflammatory activities of 71 Austrian traditional herbal drugs

Vogl

Picker

Mihály-Bison

et al. 2013

Journal of Ethnopharmacology

225

130

View full text Add to dashboard Cite

show abstract

“…Oregonin was isolated from the methanol extract of Alnus formosana leaves that was subjected to liquid-liquid partitioning. The n-butanol soluble fraction was separated on a Sephadex LH-20 column to provide an oregonin-rich fraction that was further purified by CPC to yield pure oregonin [89]. Abedini et al also aimed to optimize the isolation of oregonin from the methanol extract of A. glutinosa bark.…”

Section: Further Isolation Techniquesmentioning

confidence: 99%

“…The optimized conditions allowed as short analysis time as 6 min. Oregonin contents were examined in spiked human serum samples as well as in pig urine and dialysate samples after intragastrical administration of an oregonin-rich fraction from the leaves of A. formosana [89]. Watanabe et al developed a rapid micellar electrokinetic chromatography (MEKC) method using a high molecular mass surfactant for the separation and determination of curcumin, demethoxycurcumin, and bisdemethoxycurcumin in turmeric samples.…”

Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

Characterization of diarylheptanoids: An emerging class of bioactive natural products

Alberti

Riethmüller

Béni

2018

Journal of Pharmaceutical and Biomedical Analysis

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Diarylheptanoids are a class of secondary plant metabolites with a wide variety of bioactivity. Research on their phytochemistry and phytoanalysis is rapidly growing and the number of identified structures bearing the aryl-C-aryl skeleton is at present approaching 500. Historically, the yellow pigment curcumin has been characterized as the first diarylheptanoid and the extensive research on naturally occurring analogues is still ongoing. In this review, studies dealing with the characterization of linear and cyclic derivatives are discussed from the phytoanalytical point of view. Isolation, fractionation and purification strategies from natural sources along with their chromatographic behavior and structural characteristics are discussed. The role of various techniques used for the extraction (such as Soxhlet extraction, sonication, maceration/percolation, microwave-assisted extraction, supercritical carbon dioxide extraction); isolation (liquid-liquid extraction, column chromatographic techniques, preparative thin-layer and high-performance liquid chromatography, centrifugal partition chromatography, counter-current chromatography); separation (thin-layer chromatography, high-performance liquid chromatography, gas chromatography, capillary electrophoresis) and structural characterization (UV/Vis spectroscopy, infrared spectroscopy, X-ray crystallography, mass spectrometry, nuclear magnetic resonance spectroscopy and circular dichroism spectroscopy) are critically reviewed.

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“…It has been reported that diarylheptanoids reveal a strong antioxidant activity not only in food compositions but also in human/animal organisms providing high level of their protection [3][4]. One of the most known diarylheptanoids is 1,7-bis-(3,4-dihydroxyphenyl)-heptan-3-one-5-O-β-D-xylopyronoside or oregonin, which displayed anti-inflammatory and antioxidant activities [5][6]. Traditionally, classical Soxhlet extraction is used for isolation of extractives from plant materials.…”

Section: Introductionmentioning

confidence: 99%

Determination and Separation of Diarylheptanoids From Alder Growing in Latvia

Roze

Bikovens

Телышева

2015

ETR

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The composition of diarylhepatnoids fraction isolated from bark of two alder species (grey alder and black alder) was studied. The efficiency of three extraction methods used for isolation of diarylhepatnoids from alder bark was compared. xylopyranoside were isolated from the bark of grey alder. The phenolic components of the extracts were analyzed by high-performance liquid chromatography (HPLC). Quantitative determination of oregonin was performed using an internal standard method. The results obtained show that alder barks are rich source of diarylhepatnoids.

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Determination of oregonin in Alnus plants and biological samples by capillary electrophoresis

Cited by 11 publications

References 14 publications

Ethnopharmacological in vitro studies on Austria's folk medicine—An unexplored lore in vitro anti-inflammatory activities of 71 Austrian traditional herbal drugs

Ethnopharmacological in vitro studies on Austria's folk medicine—An unexplored lore in vitro anti-inflammatory activities of 71 Austrian traditional herbal drugs

Characterization of diarylheptanoids: An emerging class of bioactive natural products

Determination and Separation of Diarylheptanoids From Alder Growing in Latvia

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