Determination of oxytetracycline levels in rainbow trout serum on a biphenyl column using high-performance liquid chromatography

Miller, Ron; Reimschuessel, Renate; Carson, Mary C

doi:10.1016/j.jchromb.2007.01.040

Cited by 8 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A mixture of 450 μl of each plasma sample, 50‐μl internal standard doxycycline (IS) 40 μg/ml dissolved in mobile phase and 1‐ml acetonitrile was vortexed for 1 min and then centrifuged at 11,200 g for 10 min (Gelec ® Argentina) in order to separate proteins. The supernatant was evaporated under air flow in a Turbo Vap workstation (Massachusetts, USA) and resuspended in 3‐ml McIlvaine‐EDTA solution prepared according to Miller, Reimschuessel, and Carson (). Strata ® polymeric reversed phase (33 μm) 200 mg/3 ml cartridges (Phenomenex) were used for solid phase extraction under vacuum (Visiprep Supelco Vacuum Manifold) according to Fritz and Zuo ().…”

Section: Methodsmentioning

confidence: 99%

Impact of water hardness on oxytetracycline oral bioavailability in fed and fasted piglets

Decundo

Dieguez

Martínez

et al. 2019

Veterinary Medicine & Sci

View full text Add to dashboard Cite

Water hardness is a critical factor that affects oxytetracycline dissolution by chelation with cations. These interactions may lead to impaired dosing and consequently decrease absorption. Moreover, feed present in gastrointestinal tract may interact with antibiotic and alter pharmacokinetic parameters. In the present study, dissolution profiles of an oxytetracycline veterinary formulation were assessed in purified, soft and hard water. Furthermore, oxytetracycline absolute bioavailability, after oral administration of the drug dissolved in soft or hard water, was evaluated in fed and fasted piglets. A maximum dissolution of 86% and 80% was obtained in soft and hard water, respectively, while in purified water dissolution was complete. Results from in vivo study reconfirmed oxytetracycline's very low oral bioavailability. The greatest values were attained when antibiotic was dissolved in soft water and in fasted animals. Statistically significant lower absolute bioavailability was achieved when hard water was used and/or animals were fed. Moreover, Cmax attained in all treatments was lower than MIC90 of most important swine pathogens. For these reasons, the oral use of OTC formulations, that have demonstrated low oral bioavailability, should be avoided to treat systemic diseases in pigs.

show abstract

Section: Methodsmentioning

confidence: 99%

Impact of water hardness on oxytetracycline oral bioavailability in fed and fasted piglets

Decundo

Dieguez

Martínez

et al. 2019

Veterinary Medicine & Sci

View full text Add to dashboard Cite

show abstract

“…The extraction and detection method of OTC in plasma were based on a previously described method (Sun et al ., ; Miller et al ., ) with slight modifications. Briefly, 200 μL of buffalo plasma was deproteinated by adding 50 μL of 15% trichloroacetic acid in acetonitrile (ACN) in a 1 mL conic Eppendorf tube and then vortexed for 1 min.…”

Section: Mean ± Sd Values Of the Pharmacokinetic Parameters Of A Longmentioning

confidence: 99%

Disposition of a long‐acting oxytetracycline formulation in Thai swamp buffaloes (Bubalus bubalis)

Poapolathep

Wongpanit

Tanhan

et al. 2016

Vet Pharm & Therapeutics

View full text Add to dashboard Cite

The present study aimed to characterize the pharmacokinetic profile of oxytetracycline long-acting formulation (OTC-LA) in Thai swamp buffaloes, Bubalus bubalis, following single intramuscular administration at two dosages of 20 and 30 mg/kg body weight (b.w.). Blood samples were collected at assigned times up to 504 h. The plasma concentrations of OTC were measured by high-performance liquid chromatography (HPLC). The concentrations of OTC in the plasma were determined up to 264 h and 432 h after i.m. administration at doses of 20 and 30 mg/kg b.w., respectively. The C values of OTC were 12.11 ± 1.87 μg/mL and 12.27 ± 1.92 μg/mL at doses of 20 and 30 mg/kg, respectively. The AUC values increased in a dose-dependent fashion. The half-life values were 52.00 ± 14.26 h and 66.80 ± 10.91 h at doses of 20 and 30 mg/kg b.w, respectively. Based on the pharmacokinetic data and PK-PD index (T > MIC), i.m. administration of OTC at a dose of 30 mg/kg b.w once per week might be appropriate for the treatment of susceptible bacterial infection in Thai swamp buffaloes.

show abstract

“…A validated extraction procedure previously described by Miller et al (2007) was used. To monitor the accuracy of the method, rainbow trout control serum was spiked with 10.0 or 1.0 µg OTC/mL to obtain chromatographic standards (final concentrations of 1.0 and 0.10 µg OTC/mL, respectively).…”

Section: Experiments Day Actionmentioning

confidence: 99%

Oxytetracycline Pharmacokinetics in Rainbow Trout during and after an Orally Administered Medicated Feed Regimen

Miller

Pelsor

Kane³

et al. 2012

J Aqua Anim Hlth

Self Cite

View full text Add to dashboard Cite

The pharmacokinetic-pharmacodynamic predictor of antimicrobial activity for tetracyclines is reported to be the area under the concentration-time curve at steady state (AUC(ss)) divided by the minimal inhibitory concentration of the targeted pathogen. Here, we estimate AUC(ss) values for oxytetracycline (OTC) in serum of rainbow trout Oncorhynchus mykiss by using a destructive sampling study design. Seventy-two rainbow trout were fed OTC-medicated feed at 74.7 +/- 1.5 mg/kg (mean +/- SD) body weight (BW) by oral gavage for 10 consecutive days. Serum was collected from nine fish at 1, 3, 6, 8, 10, 12, 15, and 22 d after dosing began. Serum OTC concentrations were measured by high-performance liquid chromatography with a 0.01-microg/mL limit of detection. The average OTC AUC(ss) was 29.2 microg x h/mL and was estimated using nonlinear mixed-effects modeling and bootstrap resampling techniques. The elimination half-life was estimated as 85.0 h, and the fraction of steady state achieved was estimated as 0.85. The calculated AUC(ss) (24.8 microg x h/mL) following 10 d of oral dosing with 75 mg OTC/kg BW was less than the estimated AUC(ss). Results suggest that the pharmacokinetics of OTC exposure, including the AUC(ss), is better evaluated by using multiday dosimetry than by using a standard single-dose protocol.

show abstract

Determination of oxytetracycline levels in rainbow trout serum on a biphenyl column using high-performance liquid chromatography

Cited by 8 publications

References 15 publications

Impact of water hardness on oxytetracycline oral bioavailability in fed and fasted piglets

Impact of water hardness on oxytetracycline oral bioavailability in fed and fasted piglets

Disposition of a long‐acting oxytetracycline formulation in Thai swamp buffaloes (Bubalus bubalis)

Oxytetracycline Pharmacokinetics in Rainbow Trout during and after an Orally Administered Medicated Feed Regimen

Contact Info

Product

Resources

About