Determination of paeoniflorin, calycosin‐7‐O‐β‐d‐glucoside, ononin, calycosin and formononetin in rat plasma after oral administration of Buyang Huanwu decoction for their pharmacokinetic study by liquid chromatography–mass spectrometry

Abstract

The purpose of this study was to simultaneously investigate the pharmacokinetics of five bioactive compounds in rat plasma after oral administration of Buyang Huanwu decoction (BYHWD) using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS). The separations were performed on a Thermo Hypersil Gold C(18) analytical column (50 × 2.1 mm, 3 µm) with the column temperature kept at 30°C. The quantitative analysis was performed using a quadrupole mass spectrometer detector operated under selected ion monitoring mode. A linear gradient elution of A (0.1% formic acid solution) and B (100% acetonitrile) was used at a flow rate of 0.2 mL/min. The method was validated within the concentration ranges 1.8-450, 6.0-1500, 2.0-500, 1.2-300 and 1.2-150 ng/mL for paeoniflorin, calycosin-7-O-β-d-glucoside, ononin, calycosin and formononetin, respectively. The calibration curves were linear with correlation coefficients > 0.99. The lower limits of quantitations were < 6.0 ng/mL. The method was further applied to assess the pharmacokinetics of the five bioactive constituents of BYHWD in rat plasma.

HPLC–MS/MS Analysis of a Traditional Chinese Medical Formulation of Bu-Yang-Huan-Wu-Tang and Its Pharmacokinetics after Oral Administration to Rats

“…However, the other herbal ingredients of formononetin, ononin, calycosin, calycosin-7-O-β-d-glucoside and paeoniflorin presented a single and plateau absorption phase, consistent with a previous report [15]. By means of plateau absorption or multiple-peak absorption phase, these phenomenona were similar to another report on Chinese herbal compounds [25].…”

“…Moreover, the extent and rate of drug uptakes into the systemic circulation of AUC and C max for ligustilide were the lowest in BYHWT. However, the previous report did not monitor ligustilide as one of their target components [15]. In this study, we provide more detailed information to enable better understanding for the pharmacokinetic profiles of the herbal preparation BYHWT.…”

“…However, there are few reports available regarding pharmacokinetic studies of BYHWT. One report has previously described the analysis of multiple components of BYHWT in a pharmacokinetic study, with the determination of formononetin, ononin, calycosin, calycosin-7-O-β-d-glucoside and paeoniflorin by LC-MS [15]. However, dealing with just five constituents from one or two medicinal herbs is not corresponding with the characteristics of exhibiting effects on multiple targets.…”

Identification of metabolites of Buyang Huanwu decoction in rat urine using liquid chromatography–quadrupole time-of-flight mass spectrometry

“…Although the pharmacokinetic parameters of BYHWD have been investigated [12], up to now the literature data on the metabolism of BYHWD is still limited. In order to get much metabolism information of BYHWD from the complex biological matrices, it is necessary to develop effective and reliable analytical methods for detection.…”

Chromatographic Fingerprint Analysis and Characterization of Constituents in Shenqi Fuzheng Injection by HPLC-DAD-ELSD and UFLC-DAD-Q-TOF Tandem Mass Spectrometry Techniques

“…3. Comparing the retention time, UV spectra and mass spectra of the components in SFI with those of the reference standards, the peaks could be unambiguously identified as the known constituents, adenine (3) [17,18], 5-hydroxymethyl-furaldehyde (5) [19,20], calycosin-7-O-β-D-glucopyranoside (9) [21,22], lobetyolin (13) [11,23], ononin (14) [21,24], and astragaloside IV (3′) [25,26], respectively. The other components were tentatively identified according to their MS fragmentation pattern, and also by studying their UV spectral characteristics and reported literatures.…”