Determination of palmatine in canine plasma by liquid chromatography–tandem mass spectrometry with solid-phase extraction

Huang, Jianming; Wang, Guoquan; Jin, Young-Woo; Shen, Teng; Weng, Weiyu

doi:10.1016/j.jchromb.2007.04.047

Cited by 21 publications

(12 citation statements)

References 8 publications

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“…6). These results are consistent with those of palmatine (C 21 H 22 NO 4 , MW 352; Huang et al, 2007;Lu and Yang, 2004;Zhang et al, 2006). The mechanistic pathway for fragments formed may be explained according to Scheme 3.…”

Section: Identification Of Compounds 3 Andsupporting

confidence: 86%

“…It is impossible to obtain their effective constituents by traditional methods like pure compounds isolation, structure elucidation and then individual pharmacological tests. Therefore, methods to reveal the effective substances are in great demand (Ye et al, 2007). In fact, for most oral administrative TCMFs, only part of constituents successfully absorbed into blood and kept at a considerable concentration level in target organs can be responsible for the curative effects.…”

Section: Introductionmentioning

confidence: 99%

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Identification of major alkaloids and steroidal saponins in rat serum by HPLC‐diode array detection‐MS/MS following oral administration of Huangbai‐Zhimu herb‐pair Extract

Fan

Tang

et al. 2008

Biomedical Chromatography

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Huangbai-Zhimu herb-pair (HBZMHP) is a widely used Chinese traditional medicine formula in treating various diseases; however, its active components have remained unknown. In this paper, serum chemistry and combined high-performance liquid chromatography (HPLC), diode-array detection and mass-spectrometry (MS) techniques were used to study the constituents of HBZMHP extract absorbed into rat serum after oral administration. A total of nine characteristic HPLC peaks in the TIC chromatograms were identified as magnoflorine (1), menisperine (2), palmatine (3), berberine (4), timosaponin N or timosaponin E1 (5), timosaponin D (6), timosaponin BIII, anemarsaponin C or xilingsaponin B (7) timosaponin BII (8) and timosaponin AIII (9). All of the identified peaks were constituents of HBZMHP extract. The results narrow the range of active compounds to be found in HBZMHP extract, and pave the way for the follow-up action mechanism research.

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Section: Identification Of Compounds 3 Andsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Identification of major alkaloids and steroidal saponins in rat serum by HPLC‐diode array detection‐MS/MS following oral administration of Huangbai‐Zhimu herb‐pair Extract

Fan

Tang

et al. 2008

Biomedical Chromatography

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“…Although the pharmacological effects of BER and other Coptis extracts have been widely reported, information regarding their oral absorption remains to be fully elucidated. Previous pharmacokinetic studies have demonstrated that the four protoberberine alkaloids have markedly low plasma concentrations and poor oral bioavailability in rats, beagle dogs and humans following oral administration (24)(25)(26)(27). Furthermore, several studies have reported that BER is a P-glycoprotein (P-gp)…”

Section: Introductionmentioning

confidence: 99%

Poor permeability and absorption affect the activity of four alkaloids from Coptis

Cui

Zhang

Wang

et al. 2015

Molecular Medicine Reports

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Coptidis rhizoma (Coptis) and its alkaloids exert various pharmacological functions in cells and tissues; however, the oral absorption of these alkaloids requires further elucidation. The present study aimed to examine the mechanism underlying the poor absorption of alkaloids, including berberine (BER), coptisine (COP), palmatine (PAL) and jatrorrhizine (JAT). An ultra‑performance liquid chromatography (UPLC) method was validated for the determination of BER, COP, PAL and JAT in the above experimental medium. In addition, the apparent oil‑water partition coefficient (Po/w); apparent permeability coefficient (Papp), determined using a parallel artificial membrane permeability assay (PAMPA) plate; membrane retention coefficient (R %); and effect of P‑glycoprotein (P‑gp) inhibitor on the Papp of the four alkaloids were investigated. The intestinal absorption rate constant (Ka) and absorption percentage (A %) of the four alkaloids were also determined. The results of the present study demonstrated that the Po/w of the four alkaloids in 0.1 mol·l‑1 HCl medium was significantly higher (P<0.01), compared with those of the alkaloids in phosphate buffer (pH 7.4). The Papp of BER was 1.0‑1.2x10‑6 cm·s‑1, determined using a PAMPA plate, and the Papp of BER, COP, PAL and JAT decreased sequentially. The concentrations of the four alkaloids on the apical‑to‑basolateral (AP‑BL) surface and the basolateral‑to‑apical (BL‑AP) surface increased in a linear manner, with increasing concentrations between 10 and 100 µmol. In addition, the transportation of BER on the BL‑AP surface was significantly faster (P<0.01), compared with that on the AP‑BL surface and, following the addition of verpamil (a P‑gp inhibitor), the Papp (AP‑BL) of the four alkaloids increased, whereas the Papp (BL‑AP) was significantly decreased (P<0.01). The rat intestinal perfusion experiment demonstrated that the four alkaloids were poorly absorbed; however, the Ka of BER was significantly higher, compared with the three other alkaloids. Furthermore, the A % and Ka provided evidence that the absorption of BER was increased in the jejunum, compared with in the ileum. In conclusion, the four alkaloids from Coptis appeared to be poorly absorbed, determined using a shake flask, pre‑coated PAMPA plates, a Caco‑2 cell monolayer model and intestinal perfusion; however, absorption was higher in the jejunum than in the ileum. Among the four alkaloids, the permeability of BER was markedly higher than the others, and P‑gp efflux had a significant effect on the absorption of those alkaloids.

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“…The results also showed that although the chemical structures of the six alkaloids were similar, their pharmacokinetic behaviors were different. As in the literatures [18,19], the pharmacokinetic parameters after administration of BB and PM were studied, and we could found that there were more peaks in the decoction and the T max were longer than individual compounds, which meant there might be synergistic effects after administration of the decoction.…”

Section: Resultsmentioning

confidence: 90%

Pharmacochemistry and integrated pharmacokinetics of six alkaloids after oral administration of Huang-Lian-Jie-Du-Tang decoction

Yang

Zhang

et al. 2014

Journal of Asian Natural Products Research

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Pharmacochemistry and integrated pharmacokinetics of six alkaloids (groenlandicine, berberine, palmatine, epiberberine, jatrorrhizine, and columbamine) after oral administration of Huang-Lian-Jie-Du-Tang (HLJDT) decoction were investigated in this paper. The method of plasma pharmacochemistry was applied to predict the potential bioactive components in HLJDT decoction. Based on the accurate molecular weight, 10 components including 2 flavonoids (baicalin and wogonoside), 1 iridoid glycoside (geniposide), and 7 alkaloids (above-mentioned 6 alkaloids and coptisine) were structurally identified. Then, integrated pharmacokinetics of the alkaloids in Sprague-Dawley rats after oral administration of HLJDT decoction was investigated by HPLC method. The results showed that the pharmacokinetic behaviors of the alkaloids were different although their chemical structures were similar. This study developed a method to predict the potential bioactive components in HLJDT decoction and research the pharmacokinetic behaviors of the potential bioactive components.

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Determination of palmatine in canine plasma by liquid chromatography–tandem mass spectrometry with solid-phase extraction

Cited by 21 publications

References 8 publications

Identification of major alkaloids and steroidal saponins in rat serum by HPLC‐diode array detection‐MS/MS following oral administration of Huangbai‐Zhimu herb‐pair Extract

Identification of major alkaloids and steroidal saponins in rat serum by HPLC‐diode array detection‐MS/MS following oral administration of Huangbai‐Zhimu herb‐pair Extract

Poor permeability and absorption affect the activity of four alkaloids from Coptis

Pharmacochemistry and integrated pharmacokinetics of six alkaloids after oral administration of Huang-Lian-Jie-Du-Tang decoction

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