Determination of pesticides fenoxycarb and permethrin by sequential injection chromatography using miniaturized monolithic column

Chocholouš, Petr; Šatı́nský, Dalibor; Sladkovský, Radek; Pospı́šilová, Marie; Solich, Petr

doi:10.1016/j.talanta.2008.06.015

Cited by 32 publications

(14 citation statements)

References 20 publications

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“…The peak asymmetry of lignans on a particulate Ascentis Express column 1.00-1.10 was lower than that on a Chromolith column. This fi nding agrees with earlier observation found for phenol, N,N-dimethylaniline and toluene (Wu et al, 2004) and pesticides (Chocholouš et al, 2008). They showed that the tailing on monolithic columns was higher than packed columns for acidic, basic, and even neutral compounds.…”

Section: Hplcsupporting

confidence: 93%

Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application

Březinová

Vlašínová

Havel

et al. 2010

Biomedical Chromatography

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A simple and rapid method for determination of six lignans found in plant cell cultures of Schisandra chinensis was developed and validated. The lignans were extracted from plant samples with methanol and the extracts were effectively cleaned by solid-phase extraction using Strata C18-E (Phenomenex) cartridges. Chromatographic separation was carried out on a Chromolith Performance RP-18e monolithic column (100 x 4.6 mm, Merck) using an isocratic mobile phase of acetonitrile and water in a 50:50 (v/v) ratio. The eluent was monitored at 220 nm. The baseline separation of schizandrin, gomisin A, deoxyschizandrin, gamma-schizandrin, gomisin N and wuweizisu C was achieved in a relatively short time period (20 min), which was made possible by the relatively high flow rate of the mobile phase (2 mL/min). The lower limit of quantitation was 0.1 mg/L for schizandrin and gomisin A, 0.3 mg/L for deoxyschizandrin, gamma-schizandrin, and gomisin N and 1 mg/L for wuweizisu C. The analysis of spiked samples containing six lignans provided absolute recoveries between 93 and 101% in all cases. The validated method was successfully applied to the determination of lignans in embryogenic plant cell cultures of Schisandra chinensis.

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Section: Hplcsupporting

confidence: 93%

Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application

Březinová

Vlašínová

Havel

et al. 2010

Biomedical Chromatography

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“…A Cheminert® Valco10-port multi-position valve (SV) model C25 stream selector C25-3180 EMH (Valco Instruments, Houston, TX, USA) was used to select and drive sample and mobile phase solutions though the system. Port 2 of SV is connected to a 5 mm long guard column coupled to a 25 mm reverse phase C 18 Chromolith Flash monolithic column, both from Merck KGaA (Darmstadt, Germany). Detection was made by molecular absorbance spectrophotometry using an USB 4000 spectrometer (Ocean Optics, Dunedin, An LC 9A Shimadzu high performance liquid chromatograph (HPLC), equipped with a SPD 6 AV UV detector and the LC Workstation Class-LC 10 software was used in comparison studies.…”

Section: A Sichrommentioning

confidence: 99%

“…Electroanalytical methods are known to attend the demand for minimal sample treatment and low consumption of organic solvents, 12,13 but these techniques are liable to matrix effects such as passivation 15 and the low backpressures provided by C 18 monolithic stationary phases. This technique uses low cost instrumentation and has been applied especially in the determination of components of pharmaceutical products, 16 although determinations of herbicides and pesticides 17,18 and amino acids 19 have been described. The present paper shows that the SIC capabilities for separation of simple mixtures can be explored for monitoring the maximum concentration levels of picloram in natural waters.…”

Section: Introductionmentioning

confidence: 99%

“…A metodologia foi aplicada a águas de rio fortificadas, não sendo observadas diferenças estatisticamente significativas em comparação com a metodologia convencional de HPLC-UV. This paper describes a sequential injection chromatography procedure for determination of picloram in waters exploring the low backpressure of a 2.5 cm long monolithic C 18 column. Separation of the analyte from the matrix was achieved in less than 60 s using a mobile phase composed by 20:80 (v v .…”

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confidence: 99%

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Determination of picloram in waters by sequential injection chromatography with UV detection

Santos¹,

Infante²,

Masini³

2010

J. Braz. Chem. Soc.

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Este trabalho descreve um procedimento de cromatografia por injeção seqüencial para a determinação de picloram em águas explorando a baixa pressão de uma coluna monolítica C 18 de 2,5 cm de comprimento. A separação do analito da matriz foi obtida em menos de 60 s usando como fase móvel uma mistura de acetonitrila e H 3 PO 4 5,0 mmol L -1 na proporção 20:80 (v v -1 ) e vazão de 30 mL s -1. Detecção foi feita a 223 nm com uma cela de 40 mm de caminho óptico. O limite de detecção do método é adequado para monitorar o nível de concentração máximo permitido para picloram em água potável (500 mg L -1 ). A frequência de amostragem é de 60 análises por hora, consumindo 300 mL de acetonitrila por análise. A metodologia foi aplicada a águas de rio fortificadas, não sendo observadas diferenças estatisticamente significativas em comparação com a metodologia convencional de HPLC-UV. This paper describes a sequential injection chromatography procedure for determination of picloram in waters exploring the low backpressure of a 2.5 cm long monolithic C 18 column. Separation of the analyte from the matrix was achieved in less than 60 s using a mobile phase composed by 20:80 (v v ). The sampling frequency is 60 analyses per hour, consuming only 300 mL of acetonitrile per analysis. The proposed methodology was applied to spiked river water samples and no statistically significant differences were observed in comparison to a conventional HPLC-UV method.Keywords: sequential injection chromatography, monolithic column, picloram, waters IntroductionThe presence of pesticides in surface and ground waters is a consequence of the extensive use of these chemicals in agriculture and their runoff down through the soil profile. Picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxilic acid) is a herbicide widely used to control weeds in crops of sugar cane (pre-emergency), rice, pasture and wheat (pos-emergency).2 This herbicide can stay active in soil for long time, depending on the type of soil, soil moisture and temperature. It may exist at toxic levels to plants for more than one year after application at normal rates. 2,3 It is chemically adsorbed onto clay particles and natural organic matter occurring in soils. If the soil is poor in clay or organic matter contents, the herbicide may be easily leached to surface and ground waters. 3-6Determination of picloram is usually made by gas-liquid chromatography with electron capture detector or mass spectrometry detectors, 7 although several high performance liquid chromatography methods have already been proposed using either UV absorption or mass spectrometry detection modes. [8][9][10][11] These methods are very sensitive, but require the use of large sample volumes, besides to extensive extraction steps, derivatization reactions and expensive instrumentation, so that new sensitive methods that reduce the time of analysis and the use of organic solvents are needed. Electroanalytical methods are known to attend the demand for minimal sample treatment and low consumption of organic solvent...

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“…A zona de amostra transportada através da coluna monolítica pela fase móvel, sendo a detecção espectrofotométrica realizada em 210 nm. As medidas foram baseadas na altura dos picos como em procedimentos desenvolvidos anteriormente em SIC [35,50]. Todos os experimentos foram realizados em triplicata em temperatura ambiente (25ºC).…”

Section: Procedimentounclassified

Estratégias para aumento de sensibilidade e seletividade de separações cromatográficas em sistemas de análises em fluxo

Batista¹

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Determination of pesticides fenoxycarb and permethrin by sequential injection chromatography using miniaturized monolithic column

Cited by 32 publications

References 20 publications

Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application

Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application

Determination of picloram in waters by sequential injection chromatography with UV detection

Estratégias para aumento de sensibilidade e seletividade de separações cromatográficas em sistemas de análises em fluxo

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