Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application

Abstract

A simple and rapid method for determination of six lignans found in plant cell cultures of Schisandra chinensis was developed and validated. The lignans were extracted from plant samples with methanol and the extracts were effectively cleaned by solid-phase extraction using Strata C18-E (Phenomenex) cartridges. Chromatographic separation was carried out on a Chromolith Performance RP-18e monolithic column (100 x 4.6 mm, Merck) using an isocratic mobile phase of acetonitrile and water in a 50:50 (v/v) ratio. The eluent was monitored at 220 nm. The baseline separation of schizandrin, gomisin A, deoxyschizandrin, gamma-schizandrin, gomisin N and wuweizisu C was achieved in a relatively short time period (20 min), which was made possible by the relatively high flow rate of the mobile phase (2 mL/min). The lower limit of quantitation was 0.1 mg/L for schizandrin and gomisin A, 0.3 mg/L for deoxyschizandrin, gamma-schizandrin, and gomisin N and 1 mg/L for wuweizisu C. The analysis of spiked samples containing six lignans provided absolute recoveries between 93 and 101% in all cases. The validated method was successfully applied to the determination of lignans in embryogenic plant cell cultures of Schisandra chinensis.

Phytochemical and biotechnological studies on Schisandra chinensis cultivar Sadova No. 1—a high utility medicinal plant

“…Besides examining SchS raw materials for SL content, the current study involved establishing in vitro shoot cultures of the Sadova cultivar, with the aim of providing an alternative, sustainable source of the aforementioned compounds. So far, there have been only few reports on in vitro cultures of Sch, including the projects of the authors of this paper (Březinová et al 2010 ; Kohda et al 2012 ; Szopa and Ekiert 2012 ; Szopa et al 2016b ), whereas in vitro cultures of SchS has not been studied at all.…”

Accumulation of dibenzocyclooctadiene lignans in agar cultures and in stationary and agitated liquid cultures of Schisandra chinensis (Turcz.) Baill

“…13.90 mg/100 g DW), gomisin A (11.90 mg/100 g DW), and schisandrin (max. 0.90 mg/100 g DW) (Březinová et al 2010). The presence of deoxyschisandrin, gomisin G, schisantherin A, schisandrin C, schisantherin B, and schisanthenol, and other tentatively identified dibenzocyclooctadiene lignans, which were detected under our study in significant quantities, had not been revealed by Březinová’s team.…”

Current knowledge of Schisandra chinensis (Turcz.) Baill. (Chinese magnolia vine) as a medicinal plant species: a review on the bioactive components, pharmacological properties, analytical and biotechnological studies

“…A much broader scope of research is presented by the second work by scientific workers from Masaryk and Mendel Universities in Brno (Březinová et al 2010). In their study different cell lines were examined; three lines of embryogenic callus in agar cultures were maintained in the dark (lines: LA-4, LAT-52, LA-58) and five lines of suspension cultures were maintained in a photoperiod (lines: LA-1, LA-4, LA-10, LAT-52, LA-58).…”