Determination of phenolic carboxylic acids by micellar electrokinetic capillary chromatography and evaluation of factors affecting the method

Bjergegaard, Charlotte; Michaelsen, Søren; Sørensen, Hilmer

doi:10.1016/0021-9673(92)87148-2

Cited by 49 publications

(27 citation statements)

References 15 publications

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“…As expected [5], increasing buffer concentration generally induced longer migration times (MT). However, this behaviour can also be attributed to the alteration of the micellar phase, causing a decrease in the critical micelle concentration [5,9]. As already pointed out in previous works [8,9], this is largely due to the direct effect on the electroosmotic flow, which decreases as a result on the capillary wall zeta potential.…”

Section: Buffer Concentrationmentioning

confidence: 71%

Micellar electrokinetic capillary chromatography for selected tropane alkaloid analysis in plant extract

et al. 1997

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Micellar electrokinetic capillary chromatography was applied to the simultaneous analysis of six tropane alkaloids, including hyoscyamine and scopolamine. Successful results were obtained using a 30 mM boratephosphate buffer at basic pH (8.5) in the presence of 50 mM sodium dodecyl sulfate. The operating conditions, such as buffer concentration and pH, micelle concentration and organic modifier type and percentage were also discussed on the basis of the results given with a tropane alkaloid mixture. Addition of organic modifiers showed an improvement in separation efficiency and resolution. Moreover, hyoscyamine and littorine, two positional isomers, were only resolved by the addition of organic solvents such as methanol or acetonitrile. The optimized conditions were finally applied to the analysis of tropane alkaloids found in genetically transformed root cultures ofDatura candida x D. aurea

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Section: Buffer Concentrationmentioning

confidence: 71%

Micellar electrokinetic capillary chromatography for selected tropane alkaloid analysis in plant extract

et al. 1997

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“…Theory predicts that short separation times will give the highest efficiencies since diffusion is the most important feature contributing to band broadening. Increases in applied voltage lead to shorter migration times when analysing phenolic acids by MECC (Bjergegaard et a[., 1992), alkaloids by CZE (Baeyens et al, 1993), capsacinoids by MECC (Khaled et al, 1993) and glucosinolates by MECC . Reduction in migration time is highest for compounds having long MTs, meaning that the overall time of analysis is reduced at high voltage.…”

Section: Parameters Affecting Ce Separationmentioning

confidence: 99%

Capillary electrophoresis: A new technique in the analysis of plant secondary metabolites

Tomás-Barberán

1995

Phytochemical Analysis

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The possibilities and applications of capillary electrophoresis (CE) as an analytical technique for plant secondary metabolites are reviewed. Applications of both capillary zone electrophoresis and micellar electrokinetic capillary chromatography for this purpose are described. CE is compared with high pressure liquid chromato‐graphy (HPLC) as an analytical technique, and the advantages and criticisms of CE are described. The effects of the applied voltage, capillary temperature, electrolyte concentration and nature (complexing or non‐complexing buffers), buffer pH, micelle concentration and nature (sodium dodecyl sulphate or cetyltrimethylammonium bromide and the addition of organic modifiers to the running buffer (organic solvents, cyclodextrins, urea, cholate) on the parameters of separation and resolution of different secondary metabolites are discussed. The applications of CE to the analysis of flavonoid aglycones and glycosides, phenolic acids, quinones, coumarins, alkaloids, capsacinoids, glucosinolates, polyamines, monoterpenes, diterpenes, phytoecdysteroids, cardiac glycosides and saponins are shown. It is concluded that CE is a very promising analytical technique in the analysis of plant secondary metabolites, and it will become an indispensable tool, together with HPLC and gas liquid chromatography, in phytochemical laboratories since these techniques are in many ways complementary, and problems that are difficult to solve by HPLC can often be solved using CE.

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“…CZE has been used for the determination of phenolic acids in plant extracts with UV detection using the electrolyte containing phosphate and 1-propanol at pH of 7.0 [17]. Electrokinetic capillary chromatography (MECC) based on cetyltrimethylammonium bromide (CTAB) has been developed for the separation of cinnamic and benzoic acids derivatives and was successfully used for the determination of phenolic acids in plant materials [19]. Electrokinetic capillary chromatography (MECC) based on cetyltrimethylammonium bromide (CTAB) has been developed for the separation of cinnamic and benzoic acids derivatives and was successfully used for the determination of phenolic acids in plant materials [19].…”

Section: Introductionmentioning

confidence: 99%

“…Electrokinetic capillary chromatography (MECC) based on cetyltrimethylammonium bromide (CTAB) has been developed for the separation of cinnamic and benzoic acids derivatives and was successfully used for the determination of phenolic acids in plant materials [19]. Such EOF modifiers include cationic surfactants [17], metal ions [18], quaternary ammonium salts [19], and organic solvents [20], resulting in a fast migration of the anionic solutes co-migrating with EOF. The electroosmotic flow (EOF) can be reversed or reduced with EOF modifiers added to the buffer electrolyte and reversing the polarity of the power supply.…”

Section: Introductionmentioning

confidence: 99%

Separation of phenolic acids in soil and plant tissue extracts by co-electroosmotic capillary electrophoresis with direct UV detection

2000

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A capillary zone electrophoretic method for the analysis of phenolic acids in soil and plant extracts was developed with direct UV detection using a phosphate electrolyte solution. The electrophoretic separation required the phenolic acids to be charged at a pH above their pKa in order to achieve their migration towards the anode. Electroosmotic flow (EOF) was reversed in direction by adding tetraclecyhrimethylammonium bromide (gAB). Factors affecting the separation selectivib,, including the buffer pH and EOF modifiers, were investigated systematically Eight phenolic acids were separated and detected in 10 min using an electrolyte containing 25 mM phosphate, 0.5 mM gAB and 15% acetonitrile (v/v) at pH of Z20. Linear plots for the test phenolic acids were obtained in a concentration range of 0.01 -1 mM with detection limits in the range of 1.0-ZO #M. The recoveries ranged from 92.8 to 102.3% in soil and plant tissues samples spiked at 100 #M and the relative standard deviation based on the peak area were ranged 2.0 to 4.5%. The proposed method was used for the determination of phenolic acids in plant tissue and soil extracts with direct injection.

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Determination of phenolic carboxylic acids by micellar electrokinetic capillary chromatography and evaluation of factors affecting the method

Cited by 49 publications

References 15 publications

Micellar electrokinetic capillary chromatography for selected tropane alkaloid analysis in plant extract

Micellar electrokinetic capillary chromatography for selected tropane alkaloid analysis in plant extract

Capillary electrophoresis: A new technique in the analysis of plant secondary metabolites

Separation of phenolic acids in soil and plant tissue extracts by co-electroosmotic capillary electrophoresis with direct UV detection

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