Determination of phenolic compounds from oxidation of lignin lake sediments by high-performance liquid chromatography

Lobo, Ilza; Mozeto, Antônio A.; Cass, Quézia B.

doi:10.1007/bf02490996

Cited by 11 publications

(7 citation statements)

References 14 publications

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“…Linear relationship between absorption peak areas of all lignin phenols and concentration was found in the range 5.0 μM to 2.0 mM ( R 2 > 0.997). In comparison with the HPLC–DAD and GC–MS methods, the UHPSFC method exhibits relatively higher LOD. This can be attributed to the fact that despite the continuous evolvement of SFC instruments, SFC causes relatively noisier baseline than HPLC due to pressure fluctuation, mobile phase temperature effects, and modifier percentage .…”

Section: Resultsmentioning

confidence: 98%

“…Methods reported for the analysis of 11 major lignin‐derived phenols generated by CuO oxidation are based on GC–MS, HPLC with diode array detection (DAD) and CE–DAD . These methods all have different limitations: GC needs a derivatization process; the analysis time is relatively long for both GC and HPLC; CE suffers from a poor precision of retention times.…”

Section: Introductionmentioning

confidence: 99%

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Ultra‐high performance supercritical fluid chromatography of lignin‐derived phenols from alkaline cupric oxide oxidation

Sun

Lidén

Sandahl

et al. 2016

J of Separation Science

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Traditional chromatographic methods for the analysis of lignin‐derived phenolic compounds in environmental samples are generally time consuming. In this work, an ultra‐high performance supercritical fluid chromatography method with a diode array detector for the analysis of major lignin‐derived phenolic compounds produced by alkaline cupric oxide oxidation was developed. In an analysis of a collection of 11 representative monomeric lignin phenolic compounds, all compounds were clearly separated within 6 min with excellent peak shapes, with a limit of detection of 0.5–2.5 μM, a limit of quantification of 2.5–5.0 μM, and a dynamic range of 5.0–2.0 mM (R 2 > 0.997). The new ultra‐high performance supercritical fluid chromatography method was also applied for the qualitative and quantitative analysis of lignin‐derived phenolic compounds obtained upon alkaline cupric oxide oxidation of a commercial humic acid. Ten out of the previous eleven model compounds could be quantified in the oxidized humic acid sample. The high separation power and short analysis time obtained demonstrate for the first time that supercritical fluid chromatography is a fast and reliable technique for the analysis of lignin‐derived phenols in complex environmental samples.

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Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Ultra‐high performance supercritical fluid chromatography of lignin‐derived phenols from alkaline cupric oxide oxidation

Sun

Lidén

Sandahl

et al. 2016

J of Separation Science

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“…88 In some instances, isocratic elution has provided adequate resolution due to the restricted range of analytes or selectivity effects of one or more components of the mobile phase. 51,107 However, gradient elution has usually been mandatory 38,41,55,58,66,84 in recognition of the complexity of the phenolic profile of most samples. Numerous mobile phases have been employed but binary systems comprising an aqueous component and a less polar organic solvent such as acetonitrile or methanol remain common.…”

Section: Quantificationmentioning

confidence: 99%

Analytical approaches to the determination of simple biophenols in forest trees such as Acer(maple), Betula(birch), Coniferus, Eucalyptus, Juniperus(cedar), Picea(spruce) and Quercus(oak)

Bedgood

Bishop

Prenzler

et al. 2005

Analyst

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Analytical methods are reviewed for the determination of simple biophenols in forest trees such as Acer (maple), Betula (birch), Coniferus, Eucalyptus, Juniperus (cedar), Picea (spruce) and Quercus (oak). Data are limited but nevertheless clearly establish the critical importance of sample preparation and pre-treatment in the analysis. For example, drying methods invariably reduce the recovery of biophenols and this is illustrated by data for birch leaves where flavonoid glycosides were determined as 12.3 +/- 0.44 mg g(-1) in fresh leaves but 9.7 +/- 0.35 mg g(-1) in air-dried samples (data expressed as dry weight). Diverse sample handling procedures have been employed for recovery of biophenols. The range of biophenols and diversity of sample types precludes general procedural recommendations. Caution is necessary in selecting appropriate procedures as the high reactivity of these compounds complicates their analysis. Moreover, our experience suggests that their reactivity is very dependent on the matrix. The actual measurement is less contentious and high performance separation methods particularly liquid chromatography dominate analyses whilst coupled techniques involving electrospray ionization are becoming routine particularly for qualitative applications. Quantitative data are still the exception and are summarized for representative species that dominate the forest canopy of various habitats. Reported concentrations for simple phenols range from trace level (<0.1 microg g(-1)) to in excess of 500 microg g(-1) depending on a range of factors. Plant tissue is one of these variables but various biotic and abiotic processes such as stress are also important considerations.

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“…Most of the analytical protocols for furan derivatives are based on HPLC techniques with UV detection but suffer from the separation of target analytes from the complex biological matrix [20]. There are many publications investigating the phenolic compounds in plants [21][22][23][24][25]. The GC and GC-MS systems, in particular, enable the separation and identification of lignin degradation products.…”

Section: Introductionmentioning

confidence: 99%

Direct and simultaneous determination of representative byproducts in a lignocellulosic hydrolysate of corn stover via gas chromatography–mass spectrometry with a Deans switch

Zheng

Zhang

Zhao

et al. 2011

Journal of Chromatography A

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Determination of phenolic compounds from oxidation of lignin lake sediments by high-performance liquid chromatography

Cited by 11 publications

References 14 publications

Ultra‐high performance supercritical fluid chromatography of lignin‐derived phenols from alkaline cupric oxide oxidation

Ultra‐high performance supercritical fluid chromatography of lignin‐derived phenols from alkaline cupric oxide oxidation

Analytical approaches to the determination of simple biophenols in forest trees such as Acer(maple), Betula(birch), Coniferus, Eucalyptus, Juniperus(cedar), Picea(spruce) and Quercus(oak)

Direct and simultaneous determination of representative byproducts in a lignocellulosic hydrolysate of corn stover via gas chromatography–mass spectrometry with a Deans switch

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