Determination of phentolamine in blood and urine by high performance liquid chromatography

Bros, Frederic. De; Wolshin, E.M.

doi:10.1021/ac50025a038

Cited by 22 publications

(7 citation statements)

References 13 publications

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“…Phentolamine hydrochloride (Ph) and propranolol hydrochloride (Pr) (Sigma‐Aldrich) were dissolved in absolute ethanol (Eth) at a concentration of 10 mg/mL according to the manufacturer's recommendations and further diluted in 2% carboxymethylcellulose (CMC) gel (Farmácia de Manipulação Terapêutica) to obtain the following experimental dosages: Ph 0.75 µg/mL, Ph 10 µg/mL, Ph 100 µg/mL, Pr 2.5 µg/mL, Pr 10 µg/mL, and Pr 100 µg/mL. For each drug, the lowest dosage (Ph 0.75 µg/mL and Pr 2.5 µg/mL) was determined based on the minimal therapeutic plasma concentrations of phentolamine (15 ng/mL) and propranolol (50 ng/mL), respectively, and on the rate of drug release by CMC gel according to previous studies . Dosages of 10 μg/mL and 100 μg/mL were defined as intermediate and high doses for both drugs, since they were approximately 10‐ and 100‐fold higher than the lowest dosages of the drugs (Ph 0.75 µg/mL).…”

Section: Methodsmentioning

confidence: 99%

Anti‐inflammatory and anti‐resorptive efficacy of adrenergic blockers on late replanted rat incisors

Matos

Prado

Khoury

et al. 2019

Dental Traumatology

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Background/Aim: In addition to their anti-inflammatory and anti-osteoclastogenesis properties, adrenergic blockers may also have promising anti-resorptive effects that can prevent root resorption when teeth are replanted because of avulsion. The aim of this study was to investigate the effects of phentolamine (Ph) and propranolol (Pr) in gels on the repair process of late replanted rat incisors. A further aim was to evaluate the biocompatibility of both drugs to human periodontal ligament fibroblasts (HPDLFs). Materials and methods:Forty-eight maxillary right incisors were extracted from male Wistar rats, stored in paper napkins for 60 minutes, and randomly allocated to one of eight groups (n = 6). The root canal, root surface, and alveolus were treated with 0.75 µg/mL Ph gel (Ph0.75), 10 µg/mL Ph gel (Ph10), 100 µg/mL Ph gel (Ph100), 2.5 µg/mL Pr gel (Pr2.5), 10 µg/mL Pr gel (Pr10), 100 µg/mL Pr gel (Pr100), or sodium carboxymethylcellulose gel (CMC) before replantation. In the control group (CH), only the root canal was treated with calcium hydroxide paste. Thirty days following surgery, the animals were euthanized, and the right hemimaxilla was removed to perform micro-CT and histomorphometric analysis to determine osteoclastic activity.Ethanolic solutions of Ph10 and Pr10 were selected based on the in vivo study, and the viability of HPDLFs stimulated with lipopolysaccharide was determined by MTT assays. Results:The micro-CT and histomorphometric analysis revealed no significant differences among the treatments (P > .05). The presence of active osteoclasts was significantly decreased in the Ph10 and Pr10 groups (P < .05). Ph10 and Pr10 produced statistically similar cell survival rates compared to the control group (P > .05). Conclusions: Ph10 and Pr10 significantly decreased osteoclastogenesis in delayedreplanted rat teeth and were not cytotoxic toward HPDLFs. K E Y W O R D Sadrenergic blockers, phentolamine, propranolol, root resorption, tooth avulsion, tooth replantation

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Section: Methodsmentioning

confidence: 99%

Anti‐inflammatory and anti‐resorptive efficacy of adrenergic blockers on late replanted rat incisors

Matos

Prado

Khoury

et al. 2019

Dental Traumatology

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“…As we occasionally observed in patients of groups 1 and 2 that the short-acting alpha blocker phentolamine was more potent in decreasing afterload and in improving cardiac output in cases where no sufficient systemic perfusion could be obtained by sodium nitroprusside, we decided to use phentolamine as the first-line drug to reduce afterload from July 2003 onwards (group 3). Phentolamine was used for alpha blockade as it was regarded superior to phenoxybenzamine due to a much shorter halflife period [9]. With this regimen, we found an earlier lowering of AVDO 2 , indicating an earlier improvement in systemic cardiac output.…”

Section: Pharmacological Managementmentioning

confidence: 65%

The impact of afterload reduction on the early postoperative course after the Norwood operation — a 12-year single-centre experience

Furck

Hansen

Uebing

et al. 2009

European Journal of Cardio-Thoracic Surgery

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“…Several analytical methods have been explored for the detection and quantification of PA that include various Chromatographic procedures. [2][3][4][5][6][7][8][9][10][11][12][13] However, some of these techniques are highly sensitive but are tedious and require highly sophisticated instruments for routine analysis. Of the most commonly used instrumental techniques, electroanalytical approach is one of the choices for the estimation of pharmaceuticals, due to its certain advantages, such as relatively low operational cost, moderate or no sample preparation, less time consumption and due to its dynamic range offer.…”

Section: Phentolamine (Pa) 2-[[[n-(3 Hydroxyphenyl)-para-toluidion]mentioning

confidence: 99%

“…equation:64,65 E = E − E 1/2[5] Substituting all the values, 'α' was calculated to be 0.48. Furthermore, applying Laviron equation (for an irreversible anodic wave): 66 W 1/2 = 2.44RT/αnF = 62.5/αn (for the electrode process at 298 K)[6] Scheme 2.…”

mentioning

confidence: 99%

Voltammetric Determination of an Antihypertensive Agent Phentolamine at BDDE in the Presence of Surfactants

Satsangee

Jain

Shrivastava

et al. 2014

J. Electrochem. Soc.

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A new and explicit method has been developed for the determination of phentolamine (PA) at boron doped diamond electrode (BDDE) in the presence of anionic surfactant sodium lauryl sulfate using cyclic voltammetry, square-wave voltammetry and electrochemical impedance measurement. Electro-oxidation of PA exhibits a one electron coupled irreversible diffusion-controlled process. A probable oxidation mechanism has been deduced. Under the optimized condition, the response current was proportionally linear in the range of 2 to 18 ng/mL with a low detection limit of 0.71 ng/mL and quantification limit of 2.4 ng/mL. The practical applicability of the proposed method was demonstrated with successful determination of PA in human blood plasma samples.

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Determination of phentolamine in blood and urine by high performance liquid chromatography

Cited by 22 publications

References 13 publications

Anti‐inflammatory and anti‐resorptive efficacy of adrenergic blockers on late replanted rat incisors

Anti‐inflammatory and anti‐resorptive efficacy of adrenergic blockers on late replanted rat incisors

The impact of afterload reduction on the early postoperative course after the Norwood operation — a 12-year single-centre experience

Voltammetric Determination of an Antihypertensive Agent Phentolamine at BDDE in the Presence of Surfactants

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