Determination of Phosphate in Human Serum with Zirconium/ Reduced Graphene Oxide Modified Electrode

Abstract

Pencil lead with a diameter of 0.5 mm (Tombow Co., Ltd., Japan) was implanted into a Teflon tube, so only 1 cm of its tip and end were exposed (A = 0.16 cm 2). Electrical contact was made by soldering a copper wire to the end exposed side of the PGE. Pretreatment of the PGE was done by immersing for 15 min in HNO3 6 M and then washing with water. The PGE was then introduced into the electrochemical cell containing

Determination of Phosphorus

“…A zirconium-deposited graphene oxide modified pencil graphite electrode dipped in a phosphate-containing solution was used as a working electrode to measure voltammograms of 0.5 mM [Fe(CN)6] 3at pH 5; the obtained oxidation current magnitude decreased depending on the concentration of phosphate, leading to the determination of phosphate in a concentration range from 0.01 to 1 µM with RSD of 2.42%. 9 This method can determine phosphate in human serum. A microsensor of phosphate was fabricated with a micro membrane doped with bis(dibromophenylstannyl)methane as an ionophore set in a capillary.…”