Determination of phytic acid in wheat products by complete methyl esterification and liquid chromatography‐mass spectrometry analysis

Abstract

Phytic acid, the principal storage form of phosphorus in wheat, plays both beneficial and antinutrient functions for human being, and its analytical method still needs further development. In this work, we have developed a new method for the determination of phytic acid in wheat products based on derivatization with (trimethylsilyl)diazomethane in combination with liquid chromatographymass spectrometry analysis. Methyl esterification greatly decreased the polarity and the acidity of phytic acid, and thus the corresponding derivative can be easily analyzed by liquid chromatography-mass spectrometry under common conditions. Furthermore, treatment with cation exchange resin removed the polyvalent metal ions in the solutions, and thus derivatization of phytic acid can be achieved efficiently and completely. The standard curve for phytic acid has been well established in the linear range of 0.5-100 ng/mL with squared correlation coefficient more than 0.999 and the quantification limit of 0.25 ng/mL. The phytic acid content varies greatly in different wheat products, ranging from 153.5 to 17299.0 μg/g.

Rapid quantitation of trace perfluoroalkyl acids in soil by chelating resin-aided extraction and liquid chromatography-mass spectrometry analysis

“…In our previous work, it was found that adding ion exchange resins could effectively remove high valent cations from solid samples, thereby signi cantly improving the extraction e ciency of anionic phytic acid. [15] This work aimed to develop an e cient and rapid method for the determination of PFAAs in soil by chelating resin-assisted extraction.…”

Phytic acid determination in food products using the extract of rice sprout and SBA@DABCO nanoparticle-modified filter paper as a novel electrochemical biosensor

“…Therefore, the measurement of PA in food is necessary. Liquid chromatography 5,6 and spectroscopy 7,8 are current methods for PA analysis that have some disadvantages such as being timeconsuming, expensive, and complex. Thus, we have selected the simple and low-cost electrochemical method.…”