Determination of phytochemicals and antioxidant activity of methanol extracts obtained from the fruit and leaves of Tunisian Lycium intricatum Boiss.

Boulila, Abdennacer; Karim, Mattoussi; M’Rabet, Yassine; Nesrine, Rokbeni; Dhouioui, Mouna; Boussaïd, Mohamed

doi:10.1016/j.foodchem.2014.11.114

Cited by 60 publications

(50 citation statements)

References 39 publications

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“…21 Nonetheless, the enrichment of active compound(s) from bee products is considered to be simpler than that from plants. 22 Here, the cytotoxic fraction F7F-3 from T. laeviceps cerumen was found to be -mangostin (C 24 H 26 O 6 ), which belongs in the xanthone group. 23 This compound is known to have medical and pharmaceutical bioactivities, such as antioxidant, 24 antimicrobial, 25 anti-inflammatory, 26 and anticancer activities.…”

Section: Discussionmentioning

confidence: 84%

In vitro and in vivo characterization of the anticancer activity of Thai stingless bee (Tetragonula laeviceps) cerumen

Nugitrangson

Puthong

Iempridee

et al. 2015

Exp Biol Med (Maywood)

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In vitro and in vivo characterization of the anticancer activity of Thai stingless bee (Tetragonula laeviceps) cerumen AbstractTetragonula laeviceps cerumen was sequentially extracted with 80% (v/v) methanol, dichloromethane, and hexane and also in the reverse order. By the MTT assay and the respective 50% inhibition concentration value, the most active fraction was further purified to apparent homogeneity by bioassay-guided silica gel column chromatography. -Mangostin was identified by highresolution electrospray ionization mass spectrometry and nuclear magnetic resonance analyses. It had a potent cytotoxicity against the BT474, Chago, Hep-G 2 , KATO-III, and SW620 cell lines (IC 50 values of 1.22 AE 0.03, 2.25 AE 0.20, 0.94 AE 0.01, 0.88 AE 0.16, and 1.50 AE 0.39 mmol/L, respectively). The in vitro cytotoxicity of -mangostin against the five human cancer cell lines and primary fibroblasts was further characterized by real-time impedance-based analysis. Interestingly, -mangostin was more cytotoxic against the cancer-derived cell lines than against the primary fibroblasts. Later, the migration assay was performed by continuously measuring the attachment of cells to the plate electrodes at the bottom of the transwell membrane. The combined caspase-3 and -7 activities were assayed by the Caspase-Glo Õ 3/7 kit. It showed that the cytotoxic mechanism involved caspaseindependent apoptosis, while at low (non-toxic) concentrations -mangostin did not significantly alter cell migration. Furthermore, the in vivo cytotoxicity and angiogenesis were determined by alkaline phosphatase staining in zebrafish embryos along with monitoring changes in the transcript expression level of two genes involved in angiogenesis (vegfaa and vegfr2) by quantitative real-time reverse transcriptase-polymerase chain reaction. It was found that the in vivo cytotoxicity of -mangostin against zebrafish embryos had a 50% lethal concentration of 9.4 mM, but no anti-angiogenic properties were observed in zebrafish embryos at 9 and 12 mM even though it downregulated the expression of vegfaa and vegfr2 transcripts. Thus, -mangostin is a major active compound with a potential anticancer activity in T. laeviceps cerumen in Thailand.

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Section: Discussionmentioning

confidence: 84%

In vitro and in vivo characterization of the anticancer activity of Thai stingless bee (Tetragonula laeviceps) cerumen

Nugitrangson

Puthong

Iempridee

et al. 2015

Exp Biol Med (Maywood)

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“…TPC was determined using the Folin–Ciocalteu method . Gallic acid was a standard for the calibration curve ( y = 0.0049 x + 0.0368, R 2 = 0.999), and the results were expressed as grams of gallic acid equivalent (GAE) per kilogram dry weight (DW) of uncooked sand rice seeds.…”

Section: Methodsmentioning

confidence: 99%

Phenolic profile and antioxidant properties of sand rice (Agriophyllum squarrosum) as affected by cooking and in vitro digestion

et al. 2019

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BACKGROUND Sand rice (Agriophyllum squarrosum) is an underutilized pseudocereal bearing edible seeds. In this study, the phenolics and antioxidant activity of sand rice seeds after cooking and in vitro digestion were extensively investigated. RESULTS Total phenolic content (TPC) of the sand rice seeds was slightly increased whereas total flavonoid content (TFC) decreased after boiling. Furthermore, nine compounds were detected in the uncooked seeds, with hyperoside (169.19 ± 6.59 µg g−1 dry weight (DW)), protocatechuic acid (167.46 ± 7.21 µg g−1 DW), and rutin (83.15 ± 3.26 µg g−1 DW) as the major components. Apart from the bioaccessible phenolics in the aqueous fraction, these compounds retained in the solid residue of the porridge were released to varying degrees during simulated digestion. In addition, these phenolic extracts also exerted considerable antioxidant potency, which was positively correlated with their corresponding TPC, TFC, and phenolic profiles. CONCLUSION These results indicated that both boiling and in vitro digestive treatments could considerably enhance the release of bioactive compounds and thus contribute antioxidant properties to sand rice porridge. These findings suggest that sand rice seed is a potential functional food and an excellent natural antioxidant source. © 2019 Society of Chemical Industry

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“…Avocado, pineapple, banana, papaya, passion fruit, watermelon and melon are popular tropical fruits and play an important economic role in Brazil (Lv, Li, Liu, Gu, & Zhao, 2015;Silva et al, 2014;Koffi, Vroh, Baudoin, & Zoro, 2014;Contreras-Calderón, Calderón-Jaimes, Guerra-Hernández, & García-Villanova, 2011;Wang, Bostic, & Gu, 2010). An improved knowledge about these fruits' different parts (pulp, peel and seed) and chemical characterization would contribute to the use of this as a source of bioactive molecules (Abdennacer et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity, phenolics and UPLC–ESI(–)–MS of extracts from different tropical fruits parts and processed peels

Morais

Rotta

Sargi

et al. 2015

Food Research International

165

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Determination of phytochemicals and antioxidant activity of methanol extracts obtained from the fruit and leaves of Tunisian Lycium intricatum Boiss.

Cited by 60 publications

References 39 publications

In vitro and in vivo characterization of the anticancer activity of Thai stingless bee (Tetragonula laeviceps) cerumen

In vitro and in vivo characterization of the anticancer activity of Thai stingless bee (Tetragonula laeviceps) cerumen

Phenolic profile and antioxidant properties of sand rice (Agriophyllum squarrosum) as affected by cooking and in vitro digestion

Antioxidant activity, phenolics and UPLC–ESI(–)–MS of extracts from different tropical fruits parts and processed peels

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