Determination of picogram quantities of zinc in zinc metalloproteins by atomic absorption spectrometry using a graphite furnace atomizer

Kumamaru, Takahiro; Riordan, James; Vallée, Bert L.

doi:10.1016/0003-2697(82)90132-4

Cited by 12 publications

(2 citation statements)

References 6 publications

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“…Several sophisticated analytical techniques have been reported for the determination of zinc in a variety of samples such as ame atomic absorption spectrometry (FAAS), 6,7 graphite furnace atomic absorption spectrometry (GF-AAS), 8,9 inductively coupled plasma-atomic emission spectrometry (ICP-AES), 10,11 inductively coupled plasma-mass spectrometry (ICP-MS), 12,13 Xray uorescence spectrometry (XRF) 14 and voltammetry. 15,16 However, GF-AAS, ICP-AES, ICP-MS and XRF are quite expensive techniques and skilled manpower for the operation of these instruments is needed.…”

Section: Introductionmentioning

confidence: 99%

Surfactant-based dispersive liquid–liquid microextraction for the determination of zinc in environmental water samples using flame atomic absorption spectrometry

2016

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Section: Introductionmentioning

confidence: 99%

Surfactant-based dispersive liquid–liquid microextraction for the determination of zinc in environmental water samples using flame atomic absorption spectrometry

2016

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“…The furnace was programmed to stay at 150 °C for 60 s to dry samples, at 1000 °C for 60 s to clear inorganic ash, at 1800 °C for 8 s for atomization, and at 2700 °C for 5 s to clean graphite tube[44][45][46]. Purge was performed with argon gas (4.8 grade) at a flow rate of 300 mL/min.…”

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confidence: 99%

Zinc is the metal cofactor of Borrelia burgdorferi peptide deformylase

Nguyen¹,

Wu²,

Boylan³

et al. 2007

Archives of Biochemistry and Biophysics

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Peptide deformylase (PDF, E.C. 3.5.1.88) catalyzes the removal of N-terminal formyl groups from nascent ribosome-synthesized polypeptides. PDF contains a catalytically essential divalent metal ion, which is tetrahedrally coordinated by three protein ligands (His, His, and Cys) and a water molecule. Previous studies revealed that the metal cofactor is a Fe2+ ion in Escherichia coli and many other bacterial PDFs. In this work, we found that PDFs from two iron-deficient bacteria, Borrelia burgdorferi and Lactobacillus plantarum, are stable and highly active under aerobic conditions. The native B. burgdorferi PDF (BbPDF) was purified 1200-fold and metal analysis revealed that it contains approximately 1.1 Zn2+ ion/polypeptide but no iron. Our studies suggest that PDF utilizes different metal ions in different organisms. These data have important implications in designing PDF inhibitors and should help address some of the unresolved issues regarding PDF structure and catalytic function.

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Low-pressure microwave-induced helium plasma emission spectrometry: Determination of subnanogram quantities of zinc by use of a tungsten filament vaporization system

Kumamaru¹,

Riordan²,

Vallée³

1982

Analytical Biochemistry

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Determination of picogram quantities of zinc in zinc metalloproteins by atomic absorption spectrometry using a graphite furnace atomizer

Cited by 12 publications

References 6 publications

Surfactant-based dispersive liquid–liquid microextraction for the determination of zinc in environmental water samples using flame atomic absorption spectrometry

Surfactant-based dispersive liquid–liquid microextraction for the determination of zinc in environmental water samples using flame atomic absorption spectrometry

Zinc is the metal cofactor of Borrelia burgdorferi peptide deformylase

Low-pressure microwave-induced helium plasma emission spectrometry: Determination of subnanogram quantities of zinc by use of a tungsten filament vaporization system

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