Determination of plant growth regulators in pears by microwave‐assisted extraction and liquid chromatography with electrospray ionization mass spectrometry

Abstract

A new method for the determination of six plant growth regulators, 3-indolylacetic acid, 3-indolepropionic acid, 2-naphthoxyacetic acid, 2,4-dicholrophenoxyacetic acid, 1-naphthlcetic acid, and methyl naphthalene-1-acetate, in pears was established by liquid chromatography with electrospray ionization mass spectrometry. In this study, a microwave-assisted extraction technique was first applied for the determination of plant growth regulators in fruit and three cleanup techniques were, respectively, investigated for the purification of pear samples. The chromatographic separation was performed on a Diamonsil C18 column by using 0.01 mol/L formic acid/ammonium formate buffer solution (pH 3.5)/methanol (35:65, v/v) as the mobile phase with a flow rate of 0.7 mL/min in 1:1 split mode. The LODs ranged from 0.3 to 1.9 μg/kg. Under optimized conditions, the average recoveries (five replicates) for six plant growth regulators (spiked at 0.01, 0.05, and 0.5 mg/kg) ranged from 78.9 to 118.0%, and the RSDs were 1.4-10.3%.

Peer Review #1 of "Siderophore and indolic acid production by Paenibacillus triticisoli BJ-18 and their plant growth-promoting and antimicrobe abilities (v0.1)"

“…They stimulate cell division and promote cell elongation (Katzy et al, 1990;Weyers & Paterson, 2001). They are abundant in higher plants and rhizospheric microorganisms and play a vital role in plant-microbe interactions (Beneduzi et al, 2008;Costacurta & Vanderleyden, 1995;Lambrecht et al, 2000;Beck, Hansen & Lauritsen, 2003;Mao et al, 2014). Previous studies have demonstrated that Paenibacillus spp.…”

ZIF‐8@SiO2 core–shell microsphere extraction coupled with liquid chromatography and triple quadrupole tandem mass spectrometry for the quantitative analysis of four plant growth regulators in navel oranges

“…Moreover, there are interferences in fruit extract such as sugars, organic acids, pigment, and some other coextractives. So the complex sample matrix would contribute to interferences or high background noise in detection of PGRs . Therefore, it is necessary to further develop appropriate enrichment and cleanup steps before the analysis to obtain accurate analytical data.…”

Simultaneous analysis of ten phytohormones in Sargassum horneri by high‐performance liquid chromatography with electrospray ionization tandem mass spectrometry

“…HPLC–MS/MS operated in multiple reaction monitoring mode is the most commonly used technique for the highly sensitive and selective analysis of phytohormones. However, it has been mainly confined to several phytohormones from species of fruits , rice , and oilseed .…”