Determination of plasma malondialdehyde-like material and its clinical application in stroke patients.

Abstract

SpainSUMMARY Plasma malondialdehyde-like material (MDA-LM) was evaluated in 138 normal subjects and in a group of 57 stroke patients using a modification of the method of Smith et al. (1976). The basal level of MDA-LM in the control group was 35 pmol/l with a range of 22-50 pmol/l. Values above 50 timol/l were found in 80% of the patients suffering from subarachnoid haemorrhage, in 68 % of those with cerebral thrombosis, and in 17 % with transient ischaemic attacks. None of the patients with cerebral embolism, intracerebral haematoma, or lacunar infarct had values above 50 umol/l. Significant statistical differences were found between the control group and all the patients except those with lacunar infarcts.

Oxidative Stress and C-Reactive Protein in Patients with Cerebrovascular Accident ( Ischaemic Stroke ) : The Role of Ginkgo Biloba Extract

“…35 Studies evaluating markers of oxidative stress in patients in the early period of ischaemic stroke revealed an increase in the blood, cerebrospinal fluid, or salivary concentrations of lipid peroxides, protein carbonyl, homocysteine, nitric oxide, and of MDA, conjugated dienes, and other TBAreactive molecules at the onset of stroke. 8,10,33,[36][37][38][39] Some studies showed persistently elevated MDA concentrations 6 months after strokes. 40 Evaluations of antioxidants in blood, urine, or cerebrospinal fluid of ischaemic stroke patients revealed lower plasma vitamin C, E, vitamin A, and uric acid, lower glutathione peroxidase (GPX) activity, decreased glutathione (GSH) concentration, and a decreased total plasma antioxidant capacity.…”

Automation of Methods for Determination of Lipid Peroxidation

“…Using of different techniques in the determination of lipid peroxides in plasma or serum of healthy people (spectrophotometric versus HPLC method) leads to significantly different results. When using spectrophotometric techniques, the content of TBARS in plasma (serum) reached values from 0.9 to 42.7 µmol·L -1 of MDA equivalents, when HPLC technique was used, the content of TBARS in human plasma (serum) reached values of 0.6 -1.4 µmol·L -1 of MDA equivalents [78][79][80][81][82][83][84]. This was probably caused by the using different methods for modifying the preparation of plasma (serum) sample.…”

The effects of grape seed extract against toxicity of benzene on liver and kidney tissues of albino mice: biochemical evaluation

“…It was known that exposure to benzene causes oxidative stress and damage [10]. MDA one of the known secondary products of lipid peroxidation induced by oxidative damage, may be used as an indicator of cell membrane injury [11]. Due to its large role in countering increases in lipid peroxidation which occurs during oxidative stress, the response of GSH has been frequently studied.…”