Determination of plasma tranexamic acid using cation-exchange high-performance liquid chromatography with fluorescence detection

Elworthy, Paul; Tsementzis, S A; Westhead, Donald; Hitchcock, Edward

doi:10.1016/s0378-4347(00)84573-9

Cited by 31 publications

(19 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The published analytical methods in the literature include reversed-phase HPLC with ultraviolet [3,4] or fluorescent detection [5,6], and gas chromatography (GC) [7]. However, these methods involve either a pre-column [3][4][5] or post-column [7] derivatization procedure, which is very cumbersome and not suitable for large number of sample determination.…”

Section: Introductionmentioning

confidence: 99%

Liquid chromatography–tandem mass spectrometry method for the determination of tranexamic acid in human plasma

Chang

Yin

Chow

2004

Journal of Chromatography B

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Liquid chromatography–tandem mass spectrometry method for the determination of tranexamic acid in human plasma

Chang

Yin

Chow

2004

Journal of Chromatography B

View full text Add to dashboard Cite

“…Tranexamic acid has been detected using OPA. (112) Biogenic amines such as tyramine, tryptamine, and serotonin were reacted with OPA in a post- (113) or precolumn (114) mode. The OPA precolsumn fluorescent reaction has also been applied to L-buthionine-(S,R)-sulfoximine in plasma (115) and mexiletine.…”

Section: Aminesmentioning

confidence: 99%

Chemical Reagents and Derivatization Procedures in Drug Analysis

Danielson

Gallagher

Bao

2008

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

This article describes both pre‐ and postcolumn derivatization chemistry used in conjunction with either chromatography or capillary electrophoresis (CE) to facilitate the determination of drugs. Generally, only prederivatization is used in gas chromatography (GC), principally to enhance the volatility, temperature stability, and/or detectability. The GC section considers derivatization of drugs by reagent class: alkylation, acylation, and silylation. The GC sample‐handling section describes an approach that combines the extraction and derivatization steps together. Both pre‐ and postcolumn derivatization are common approaches for high‐performance liquid chromatography (HPLC) and many of these methods have been adapted for CE. Derivatization for HPLC is often directed toward aliphatic amines, carboxylic acids, or alcohols that are difficult to detect at low levels by absorbance, luminescence, or electrochemical means. In addition, small hydrophilic molecules upon prederivatization are often converted into larger more hydrophobic compounds, making reversed‐phase HPLC easier or even feasible. The HPLC section considers derivatization of drugs based on the following types: alkaloids, amines, antibiotics, barbiturates, carbonyl compounds and carboxylic acids, catecholamines, hydroxy compounds, steroids, and sulfur compounds. For the GC and HPLC sections, tables are included, giving the structures of the more important derivatizing agents, the analytes, and the corresponding reaction products. A brief rationale for derivatization chemistry with CE concludes this article. For CE, derivatization is often done to improve detectability as the path length for absorbance detection is very short and fluorescence can be effective using laser‐based systems.

show abstract

“…Plasma levels of 515-547 mM were reported [18] 5 min after intravenous bolus of 1000 mg TA. The proposed method seems simple for monitoring TA in plasma since HPLC analysis of TA in blood usually needs complicate procedures [19,20].…”

Section: Applicationmentioning

confidence: 99%

An ionizable chromophoric reagent for the analysis of primary amine-containing drugs by capillary electrophoresis

Lin

Kou

et al. 2005

Electrophoresis

View full text Add to dashboard Cite

We found that ofloxacin acyl chloride is a potential chromophoric reagent for labeling amino analytes for capillary electrophoresis. Ofloxacin acyl chloride has a tertiary amino function in its structure and the derivatives from ofloxacin acyl chloride reacting with amino analytes can be ionized by an acid treatment and analyzed by simple capillary zone electrophoresis. Ofloxacin acyl chloride was used to derivatize model analytes (without chromophore) of amantadine (amino drug), tranexamic acid (non-protein amino carboxylic acid), glycine, and methionine (protein amino acids). The resulting derivatives were analyzed by capillary zone electrophoresis with ultraviolet detection (300 nm). The detection limits of the analytes studied were in the range of 1.0-2.5 microM (S/N = 3, injection 3 s). The precision (relative standard deviation) and accuracy (relative error) of the method for intra- and inter-day analyses of the analytes were respectively below 4.5% and 3.9%. Application of the method to the analysis of tranexamic acid in plasma proved feasible.

show abstract

Determination of plasma tranexamic acid using cation-exchange high-performance liquid chromatography with fluorescence detection

Cited by 31 publications

References 18 publications

Liquid chromatography–tandem mass spectrometry method for the determination of tranexamic acid in human plasma

Liquid chromatography–tandem mass spectrometry method for the determination of tranexamic acid in human plasma

Chemical Reagents and Derivatization Procedures in Drug Analysis

An ionizable chromophoric reagent for the analysis of primary amine-containing drugs by capillary electrophoresis

Contact Info

Product

Resources

About