Determination of plasma vitamin C concentration in fattening cattle

Lin, Haiying; Padilla, L.; Yoshimatsu, Kazutaka; Matsui, Tohru; Kitagawa, Masayuki; Yano, Hideo

doi:10.1046/j.1344-3941.2003.00079.x

Cited by 8 publications

(9 citation statements)

References 12 publications

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“…Elution was isocratic at a flow rate of 0.9 ml/min and the eluent was monitored at 265 nm to measure AsA. We previously confirmed that the treatment with dithioerythritol completely converted dehydroascorbic acid to AsA and stabilized AsA in plasma samples for 1 week at -20°C [9].…”

Section: Animals and Sample Collectionmentioning

confidence: 82%

“…Plasma vitamin C levels in calves showed great individual differences with variations related to genetic background [13]. We also reported a large individual difference of plasma vitamin C levels in beef cattle [9]. The large individual difference of plasma vitamin C might mask the relationship between plasma vitamin C and BHBA.…”

Section: Discussionmentioning

confidence: 97%

“…Therefore the sum of ASA and dehydroascorbic acid shows vitamin C status in the body. We prepared plasma samples and determined vitamin C (AsA + dehydroascorbic acid) by the method of Haiying et al [9]. Briefly, blood samples were stored on ice and centrifuged at 4°C at 3,000 rpm.…”

Section: Animals and Sample Collectionmentioning

confidence: 99%

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Plasma Vitamin C Concentration is Not Related to the Incidence of Ketosis in Dairy Cows during the Early Lactation Period

Padilla

Shibano²,

Inoue³

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. Many animals including cattle can synthesize vitamin C from glucose. The objective of this study was to investigate plasma vitamin C concentration in ketotic cows during the early lactation period because glucose supply for vitamin C synthesis might be limited in these cows. We measured plasma β -hydroxybutyrate (BHBA) concentration in 118 cows within 2 months after parturition. Subclinical/clinical ketosis was quantitatively determined using a plasma BHBA threshold of 1,200 µM. Plasma glucose concentration was lower in the ketotic cows than in the control cows but plasma vitamin C concentration did not differ between the control and the ketotic cows. Then we measured plasma vitamin C, BHBA and glucose levels in 7 cows during the periparturient period. Plasma BHBA increased and plasma glucose decreased after parturition but plasma vitamin C did not change. These results indicate that plasma vitamin C is not related to the incidence of ketosis in the early lactation period. We suggest that ketotic cows have the ability to produce vitamin C to meet its requirement in the early lactation period although glucose supply is not sufficient for milk production. Vitamin C synthesis is possibly given a high metabolic-priority for glucose in lactating cows.KEY WORDS: dairy cow, β -hydroxybutyrate, ketosis, vitamin C.

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Section: Animals and Sample Collectionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 97%

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Plasma Vitamin C Concentration is Not Related to the Incidence of Ketosis in Dairy Cows during the Early Lactation Period

Padilla

Shibano²,

Inoue³

et al. 2005

The Journal of Veterinary Medical Science

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“…Urine samples collected in each period were pooled before analyses. Plasma and urinary VC concentrations were determined as ascorbic acid by HPLC (Haiying et al, 2003;Kashiba et al, 2002) within 4 d of sampling.…”

Section: Methodsmentioning

confidence: 99%

The effect of vitamin C supplementation on plasma concentration and urinary excretion of vitamin C in cattle

Padilla

Matsui

Ikeda

et al. 2007

Journal of Animal Science

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We investigated the plasma concentration and urinary excretion of vitamin C in cows supplemented with vitamin C. Five cows (mean BW = 597 kg) were allocated to a 5 x 5 Latin square design and supplemented with a vitamin C preparation coated with hydrogenated soybean oil at 0, 10, 20, 40, or 60 mg of vitamin C per kg of BW per day for 9 d. Plasma and urine samples were collected for measuring vitamin C concentration. Urinary excretion of vitamin C was expressed as the ratio of vitamin C to creatinine. Plasma vitamin C concentration and urinary vitamin C excretion increased quadratically as dietary vitamin C increased (P < 0.001); that is, the lowest dose affected neither plasma vitamin C concentration nor urinary vitamin C excretion but the plasma vitamin C concentration and urinary vitamin C excretion increased (P < 0.05) with increasing supplementation of vitamin C at greater doses. This suggests that plasma vitamin C concentration affects urinary excretion of vitamin C in cattle and that plasma vitamin C concentration exceeded the renal threshold for vitamin C in the cows receiving vitamin C at 20 mg/kg of BW per day. Furthermore, increased urinary excretion of vitamin C appears to limit plasma vitamin C concentration in response to vitamin C intake. The daily excretion of vitamin C was estimated by the reported value of daily creatinine excretion, indicating that the daily amount of vitamin C excreted into urine was more than half of supplied vitamin C. Therefore, a large part of supplied vitamin C probably escapes ruminal degradation and is absorbed but excreted into urine.

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“…We prepared plasma samples and determined the concentration of vitamin C (AsA+ dehydroascorbic acid) as described by Haiying et al (2003).…”

Section: Chemical and Statistical Analysesmentioning

confidence: 99%

Heat stress decreases plasma vitamin C concentration in lactating cows

et al. 2006

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Determination of plasma vitamin C concentration in fattening cattle

Cited by 8 publications

References 12 publications

Plasma Vitamin C Concentration is Not Related to the Incidence of Ketosis in Dairy Cows during the Early Lactation Period

Plasma Vitamin C Concentration is Not Related to the Incidence of Ketosis in Dairy Cows during the Early Lactation Period

The effect of vitamin C supplementation on plasma concentration and urinary excretion of vitamin C in cattle

Heat stress decreases plasma vitamin C concentration in lactating cows

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