Determination of polyamines in human urine by precolumn derivatization with benzoyl chloride and high-performance liquid chromatography coupled with Q-time-of-flight mass spectrometry

Multi-Perfluoroalkyl Derivatization of Polyamines for Selective Liquid Chromatography-Tandem Mass Spectrometric Analysis Utilizing Fluorous Affinity

“…The LODs of Put, Cad, Spd, and Spm were 0.72, 0.31, 0.63, and 1.4 nM (corresponding to 3.3, 1.4, 2.9, and 6.5 fmol on column), respectively. These values were similar to those reported in previous studies employing derivatization with conventional ESI-amenable reagents for sensitive analysis of polyamines [9][10][11][12][13][14]. The LLOQs of Put, Cad, Spd, and Spm were 2.3, 1.0, 2.1, and 4.6 nM (corresponding to 10, 4.7, 9.6, and 21 fmol on column), respectively.…”

“…The basal levels of polyamine concentrations in normal cells are highly regulated by biosynthetic enzymes; therefore, determining their endogenous concentrations is well-known method for diagnosing a number of cancer and ageassociated diseases because the concentrations in neoplastic cells are higher than those in normal cells [3,4]. Typical analytical approaches for the determination of polyamines have employed liquid chromatography (LC) separation with optical or mass spectrometric (MS) detection [5][6][7][8][9][10][11][12][13][14]. Many of these methods require derivatization of the polyamines to be analysed.…”

“…For LC-MS analysis, these derivatization methods have been also useful because the high ionization efficiency of the obtained derivatives allows sensitive detection with an electrospray ionization (ESI)-MS source. Moreover, their moderate retention and separation on reversed-phase LC columns were obtained with appropriate mobile phases [9][10][11][12][13][14].…”

Determination of polyamine metabolome in plasma and urine by ultrahigh performance liquid chromatography–tandem mass spectrometry method: Application to identify potential markers for human hepatic cancer

“…Akiko Kubo et al had combined matrix-assisted laser desorption/ionization time-offlight imaging mass spectrometry (MALDI-TOF-IMS) and capillary electrophoresis-mass spectrometry (CE-MS) to determine the apparent contents of individual polyamine metabolites in serial tissue sections [20]. We recently reported an HPLC/Q-TOF MS method for determination of six polyamines using benzoyl chloride for derivatization in human plasma and urine [21][22][23]. The improvements in this paper have been considered for analyzing the polyamine metabolome and monitoring the alteration of polyamine metabolome in different biofluids from cancer patients and healthy volunteers.…”

Isolation of Biogenic Amines Using Paramagnetic Microparticles Off-Line Coupled with Ion Exchange Liquid Chromatography

“…Determination of biogenic amines is usually carried out using thin-layer chromatography [14], gas chromatography, capillary zone electrophoresis [15], ion exchange liquid chromatography (IEC) [16], enzyme immunoassay [17] and/ or high-performance liquid chromatography [18,19]. In our study, we decided to use IEC with post-column ninhydrin derivatization and Vis detection for biogenic amines separation and detection, as they minimize the sample precleaning requirement [20].…”