Determination of polycyclic aromatic hydrocarbons in aqueous samples by reversed-phase performance liquid chromatography

Ogan, Kenneth; Katz, Elena; Slavin, Walter

doi:10.1021/ac50044a044

Cited by 124 publications

(24 citation statements)

References 13 publications

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“…The transmittance of the interference filters was accounted for in the selection of excitation and fluorescence wavelengths. Compared with detection limits by conventional HPLC fluorescence spectrophotometric detectors, the present values of 0.005-0.2 ng are better or at least comparable to those of previous HPLC spectrofluorometric detectors (IO,(17)(18)(19)(20). The spectrum of the Xe lamp was not considered in the choice of detection wavelengths.…”

Section: Wavelengths Of the Multiwavelength Fluorescencesupporting

confidence: 70%

Development of sensitive multiwavelength fluorescence detector system for high-performance liquid chromatography

Toko¹,

Glick²,

Smith³

et al. 1987

Anal. Chem.

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Cu(I1) and the other with 5% Cu(I1). The response of the calcein blue reagent to these solutions was obtained, and the Stern-Volmer quenching parameters thus measured are collected in Table IV. It was concluded that the addition of 2% Cu(I1) yielded only a 0.4% error in computed Gd(II1) concentrations, while the addition of 5% Cu(I1) yielded only a 1.3% error.Apparent association constants for the transition-metal complexes with calcein blue and methyl calcein blue were computed from the quenching data by using the same analysis c o 24 060 a 380 as previously described. These constants are found in Table V, and comparison with analogous iminodiacetate and amino acid complexes (9) indicates that the dye complexes are less stable than those of the parent chelating group. This observation implies that the steric bulk of the umbelliferone group interferes with the complexation process, as had been inferred for the lanthanide-dye complexes. LITERATURE CITEDHowell, J. A.; Hargis, L. G. Anal. Chem.

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Section: Wavelengths Of the Multiwavelength Fluorescencesupporting

confidence: 70%

Development of sensitive multiwavelength fluorescence detector system for high-performance liquid chromatography

Toko¹,

Glick²,

Smith³

et al. 1987

Anal. Chem.

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“…As in the case of the PAH, the a values for the longer solutes (i.e., based on length-to-breadth ratios) tend to increase significantly with increasing surface coverage (e.g., compound no. 35 with the parent PAH the longer isomers have increasing a values with incrleasing surface coverage. As reported previously ( I ) , polymeric CI8 materials generally provide more resolution of individual methyl isomers than do the monomeric materials.…”

Section: Resultsmentioning

confidence: 91%

“…(35) for the separation of the 16 priority pollutant PAH and the study of Amos(8), dibenz[a,h]anthracene eluted prior to benzo[ghi]perylene, whereas Wise et al (4) and Colmsjo and MacDonald (7) showed chromatograms in which benzo-[ghilperylene eluted first. Presumably, Wise et al (4) and Colmsjo and MacDonald (7) used columns from lob with high surface coverage, whereas Ogan et al (359 and Amos…”

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confidence: 99%

Effect of C18 surface coverage on selectivity in reversed-phase liquid chromatography of polycyclic aromatic hydrocarbons

Wise¹,

May²

1983

Anal. Chem.

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1479 (15) Colby, B. N.; Rosecrance, A. E.; Colby, M. E. Anal. Chem. 1981, 53, (16) Glaser, J. A.; Foerst, D. L.; McKee, G. D.; Quave, s. A,; Budde, W. L. (13) Budde, W. L.: Eichelberger, J. W. "Performance Tests for the Evaluament and Laboratories"; Environmental Protection Agency Report EPA-600/4-80-025, April 1980. (14) Sauter, A. D.; Betowskl, L. D.: Smith, T. R.; Strickler, V. A,; Belmer, R. G.; Colby, B. N.; Wllkinson, J. E. HRC CC J . High Resolut. Chromatogr. Chromatogr. Commun, 1981, 4 , 366-384. tion of Computerized Gas ChromatographylMass Spectrometry Equip-1907-1 91 1 I Envrron. Sci. Techno/. 1981, 15, Recent studles trave reported selectlvlty dlfferences for POlycyclic aromatllc hydrocarbons (PAH) on dlff erent octadecylsllane (C18) reversed-phase llquld chromatographlc columns. I n this paper the differences in llquld chromatographic selectlvllty for various Cts columns were correlated with dlfferences In C,, surface coverage data (as determlned from elemental rinalysis and specific surface area measurements on the C,, modlfled slllca). Slgnlflcant dlfferences In selectlvlty were observed for selected PAH and polyphenyl arenes on monornerlc vs,, polymeric C,, materials. Selectlvlty factors for 50 PAH, alkyksubstltuted PAH, polyphenyl arenes, and polycycllc aromatic sulfur heterocycles were determlned on columns from the same manufacturer from seven dlfferent lots of C,, material with dlfferent C,, surface concentratlons.I n addltlon to priovldlng information concerning the contrlbutlon of C18 surface coverage to retentlon rind selectlvlty In the LC separatlon olf these compounds, these studies provide useful Information regarding the selectlon of the approprlate column to optlniize the separatlon of 'selected polycycllc aromatlc compounds.Reversed-phase liquid chromatography (LC) on chemically bonded octadecylsilane (C18) stationary phases is by far the most popular LC mode for the separation of polycyclic aromatic hydrocarbons (PAH). Reversed-phase LC on Cls phases provides excellent selectivity for the separation of PAH isomers and alkyl-substituted PAH isomers ( I ) . Recently, reversed-phase LC on C18 columns has been specified as the method of choice for the analysis of aqueous effluents for the determination of the 16 PAH on the U.S. Environmental Protection Agency's priority pollutant list (2). In addition to the environmental interest in the determination of PAN by LC, PAH are excellent nonpolar solutes for use in the investigation of reversed-phase LC retention mechanisms.Since Schmit et al. (3) i h t described the separation of PAN on a chemically bonded CI8 phase in 1971, numerous reports of LC retention data for PAH on various C18 columns have appeared. Recent studies by Wise et al. (4), Ogan and Katz (5,6), Colmsjo arid MacDonald (7), and Amos (8) found that Cl8 columns from various manufacturertr not only provided different separation efficiencies but often provided different selectivities and iretention characteristics for PAH. In these studies, attention was focused on three groups of...

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“…In the second article, Katz and Ogan (12) observed that the selectivity factors for several pairs of PAHs differed significantly on the HC-ODS compared to other commercial columns and that the selectivity factors for these same PAH pairs also varied slightly for different production lots of the material used for the HC-ODS columns. Of particular interest and concern was the selectivity factor for dibenzo[a,h]anthracene (DBahA) and benzo[ghi]perylene (BghiP) since these two priority pollutant PAHs were not completely baseline resolved in their previously reported method (10). By monitoring α DBahA/BghiP and α for a second PAH pair, benzo[e]pyrene and benzo[b]fluoranthene, they defined a range of α values for these two PAH pairs that provided the appropriate separation of the 16 EPA PAHs for their method.…”

Section: Focus On Isomer Separationsmentioning

confidence: 99%

Analytical Methods for Determination of Polycyclic Aromatic Hydrocarbons (PAHs) — A Historical Perspective on the 16 U.S. EPA Priority Pollutant PAHs

Wise

Sander

Schantz

2015

Polycyclic Aromatic Compounds

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The identification of 16 polycyclic aromatic hydrocarbons (PAHs) as priority pollutants by the U.S. Environmental Protection Agency (EPA) in 1976 has been a primary driver for analytical methods development for the determination of PAHs. In this article, the historical development of methods in liquid chromatography (LC) and gas chromatography (GC) to separate these 16 PAHs is discussed. In LC a significant effort was the search for and the fundamental understanding of the unique stationary phase capable of achieving the desired separation of the 16 EPA PAHs. For GC methods, the focus on stationary phase development has been the separation of critical isomers with a broader scope than the 16 EPA PAHs. The current routine LC and GC methods for the 16 EPA PAHs are well established; however, new advances in analytical techniques beyond LC and GC are discussed. Many analysts are now interested in more than just the 16 EPA PAHs (e.g., higher molecular mass PAHs and alkyl-substituted PAHs) and analytical methods have emerged to address these needs. Reference materials and their use in the determination of PAHs are discussed.

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Determination of polycyclic aromatic hydrocarbons in aqueous samples by reversed-phase performance liquid chromatography

Cited by 124 publications

References 13 publications

Development of sensitive multiwavelength fluorescence detector system for high-performance liquid chromatography

Development of sensitive multiwavelength fluorescence detector system for high-performance liquid chromatography

Effect of C18 surface coverage on selectivity in reversed-phase liquid chromatography of polycyclic aromatic hydrocarbons

Analytical Methods for Determination of Polycyclic Aromatic Hydrocarbons (PAHs) — A Historical Perspective on the 16 U.S. EPA Priority Pollutant PAHs

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