Determination of polyphenols and free radical scavenging activity of Tephrosia purpurea linn leaves (Leguminosae)

Abstract

Background:Leaves of Tephrosia purpurea Linn. (sarpankh), belonging to the family Leguminaceae, are used for the treatment of jaundice and are also claimed to be effective in many other diseases. This research work was undertaken to investigate the in vitro antioxidant activity of aqueous and ethanolic extracts of the leaves.Method:The therapeutic effects of tannins and flavonoids can be largely attributed to their antioxidant properties. So, the quantitative determinations were undertaken. All the methods are based on UV-spectrophotometric determination.Result:The total phenolic content of aqueous and ethanolic extracts showed the content values of 9.44 ± 0.22% w/w and 18.44 ± 0.13% w/w, respectively, and total flavonoid estimation of aqueous and ethanolic extracts showed the content values of 0.91 ± 0.08% w/w and 1.56 ± 0.12%w/w, respectively, for quercetin and 1.85 ± 0.08% w/w and 2.54 ± 0.12% w/w, respectively, for rutin. Further investigations were carried out for in vitro antioxidant activity and radical scavenging activity by calculating its percentage inhibition by means of IC50values, all the extracts’ concentrations were adjusted to fall under the linearity range and here many reference standards like tannic acid, gallic acid, quercetin, ascorbic acid were taken for the method suitability.Conclusion:The results revealed that leaves of this plant have antioxidant potential. The results also show the ethanolic extract to be more potent than the aqueous decoction which is claimed traditionally. In conclusion, T. purpurea Linn. (Leguminosae) leaves possess the antioxidant substance which may be responsible for the treatment of jaundice and other oxidative stress-related diseases.

Biogenic silver nanoparticles from Abutilon indicum: Their antioxidant, antibacterial and cytotoxic effects in vitro

“…Hydrogen peroxide (H 2 O 2 ) radical scavenging activity of AIAgNPs and AILE (10-60 g/mL) was quantified by the method devised by Avani Patel et al [26]. The total reducing power of AIAgNPs and AILE (50-300 g/mL) was estimated by following the method devised by Makari et al [27].…”

GC-MS Analysis and Antioxidant Activity of Bauhinia nakhonphanomensis Leaf Ethanolic Extract

“…Therefore, the pres ent study aims to investigate the presence of phy tochemicals in an ethanolic extract of the leaves Determination of total flavonoid content Total flavonoid contents were measured using an aluminum chloride colorimetric assay. 12 The extract (concentration 1 mg/ml) was mixed with 200 µl of distilled water and 100 µl of 5 % NaNO 2 solution. After 6 min, 200 µl of 10 % AlCl 3 solution was added.…”

Evaluation of antioxidant activity of seed of Holarrhena antidysenterica: An approach through different in vitro models

“…[2] Antioxidant terminates these chain reactions by removing free radical intermediates and inhibits other oxidation reactions by being oxidized themselves. [3] Chemicals like sulfur dioxide, tert-butyl hydroxytoluene (BHT), tert-butyl-4-hydroxy anisole (BHA) and propyl galate (PG) can be used as antioxidant agents, but the use of these chemicals is restricted due to their side effects. [4] So, at present, special emphasis has been laid by many scientists on medicinal plants as an alternative therapy.…”