Background:Leaves of Tephrosia purpurea Linn. (sarpankh), belonging to the family Leguminaceae, are used for the treatment of jaundice and are also claimed to be effective in many other diseases. This research work was undertaken to investigate the in vitro antioxidant activity of aqueous and ethanolic extracts of the leaves.Method:The therapeutic effects of tannins and flavonoids can be largely attributed to their antioxidant properties. So, the quantitative determinations were undertaken. All the methods are based on UV-spectrophotometric determination.Result:The total phenolic content of aqueous and ethanolic extracts showed the content values of 9.44 ± 0.22% w/w and 18.44 ± 0.13% w/w, respectively, and total flavonoid estimation of aqueous and ethanolic extracts showed the content values of 0.91 ± 0.08% w/w and 1.56 ± 0.12%w/w, respectively, for quercetin and 1.85 ± 0.08% w/w and 2.54 ± 0.12% w/w, respectively, for rutin. Further investigations were carried out for in vitro antioxidant activity and radical scavenging activity by calculating its percentage inhibition by means of IC50values, all the extracts’ concentrations were adjusted to fall under the linearity range and here many reference standards like tannic acid, gallic acid, quercetin, ascorbic acid were taken for the method suitability.Conclusion:The results revealed that leaves of this plant have antioxidant potential. The results also show the ethanolic extract to be more potent than the aqueous decoction which is claimed traditionally. In conclusion, T. purpurea Linn. (Leguminosae) leaves possess the antioxidant substance which may be responsible for the treatment of jaundice and other oxidative stress-related diseases.

Introduction:Metriviv syrup is a poly-herbal formulation used as uterine tonic and for treating gynecological ailments such as infertility, leucorrhea, and menstrual disorders. There is no scientific data on the safety of this formulation available, therefore, its detail toxicity study in female albino rats was conducted.Aims and Objective:Acute toxicity and repeated dose 28 days oral toxicity study of metriviv syrup in female rats.Material and Methods:In oral acute toxicity study, syrup metriviv was administered orally once only at the dose of 2000mg/kg in rats in a sequential manner. For repeated dose toxicity study, Metriviv syrup was administered orally for 28 consecutive days in albino rats at three dose levels, i.e., TED, TEDx5 and TEDx10 dose levels (1.35, 0.75, 13.5 ml/kg, twice a day respectively).Results and Observation:Results of acute toxicity study showed that drug did not produce any behavioral changes, signs of toxicity and mortality up to the dose of 2000 mg/kg in rats. In repeated dose 28-day oral toxicity study, Metriviv did not produce any signs of toxicity and changes in behavioral parameters. Metriviv did not affect cellular as well as non-cellular elements of the blood to significant extent. Serum biochemical profile and histological study clearly indicated that the test formulation is not likely to produce any serious changes at lower dose level while produced mild-to-moderate changes at TEDx10 dose level and same was reverted in recovery study after discontinuation of the drug.Conclusion:The study concluded that metriviv syrup is safe to administer up to dose of 2000 mg/kg in female rats during acute toxicity. In repeated dose 28-day oral toxicity study, test drug at TEDx10 has only mild-to-moderate adverse potential for liver and kidney while did not have any major toxic effects at therapeutic dose level in female albino rats.

Background:Trisama is an Ayurvedic formulation that can be prescribed for wide range of disorders, especially abdominal disorders such as indigestion, constipation and flatulence. Trisama is prepared by mixing an equal quantity of powder of Shunthi (Zingiber officinale Linn.), Haritaki (Terminalia chebula Retz.) and Guduchi (Tinospora cordifolia [Willd.] Miers.). As a single ingredient, Haritaki is an alternative to prokinetic drugs, Guduchi for intestinal motility and Shunthi for digestion-related problems.Materials and Methods:The present study aims to evaluate the effect of Trisama dosage forms on intestinal motility by adopting kaolin expulsion test in Swiss albino mice. Trisama powder (0.65 and 1.3 g/kg) and Trisama decoction (12.48 ml/kg) were administered and intestinal transit time of kaolin and latency of onset of kaolin expulsion in fecal matter was assessed.Results:Both the dosage forms of Trisama shortened the intestinal transit time of kaolin. However, the observed effects were statistically significant in Trisama powder at higher dose and Trisama decoction in comparison to control group. The behaviors of mice and consistency of fecal pellet were almost the same as observed in normal control group.Conclusion:From the present study, it is concluded that Trisama has significant intestinal motility-enhancing property in mice which may be useful in gastric problems without affecting the general physiology.

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Indian system of medicine has a longstanding history of using medicinal plants for the prevention and treatment of various health ailments. Trisama is an ancient Ayurvedic preparation can be prescribed for a wide range of disorder but not popular as many other Ayurvedic herbal formulations. Trisama is preparing by mixing the Sunthi (Zingiber officinale Linn.), Haritaki (Terminalia chebula Retz.), and Guduchi (Tinospora cordifolia Willd Meirs) in equal proportion. It is credited with diverse beneficial therapeutic effects mainly as Shothhara and Amapachana and reported to possess many pharmacological properties due to its common Usna Virya and Madhur Vipaka of the individual ingredients. The reported pharmacological effects of its ingredients are anti-oxidant, anti-inflammatory, anti-pyretic, analgesic, antimicrobial, hypolipidemic, cardioprotective, gastroprotective, cytoprotective effects etc. The bioactive components responsible for these therapeutic potential are mainly due to the presence of major important phytoconstituents such as phenolics components like gingerols, gingerdiols and gingerdione, shogaols, zingiberine, giloin, berberine, tinosporin, tinosporin acid and gallic acid. The present review focuses on summarizing the formulation details by their Ayurvedic properties, phytoconstituents and pharmacological activity. The review suggests that, Trisama formulation is easily available, economical, palatable and more beneficial for human health.Hence, it may be popularize among general Practitioner as an important Ayurvedic medicine in the form of churna (powder) and kwatha (decoction) for the treatment of Sotha and metabolic disorders.