Assessment of Trisama, an ayurvedic formulation on intestinal transit time in swiss albino mice

Abstract

Background:Trisama is an Ayurvedic formulation that can be prescribed for wide range of disorders, especially abdominal disorders such as indigestion, constipation and flatulence. Trisama is prepared by mixing an equal quantity of powder of Shunthi (Zingiber officinale Linn.), Haritaki (Terminalia chebula Retz.) and Guduchi (Tinospora cordifolia [Willd.] Miers.). As a single ingredient, Haritaki is an alternative to prokinetic drugs, Guduchi for intestinal motility and Shunthi for digestion-related problems.Materials and Methods:The present study aims to evaluate the effect of Trisama dosage forms on intestinal motility by adopting kaolin expulsion test in Swiss albino mice. Trisama powder (0.65 and 1.3 g/kg) and Trisama decoction (12.48 ml/kg) were administered and intestinal transit time of kaolin and latency of onset of kaolin expulsion in fecal matter was assessed.Results:Both the dosage forms of Trisama shortened the intestinal transit time of kaolin. However, the observed effects were statistically significant in Trisama powder at higher dose and Trisama decoction in comparison to control group. The behaviors of mice and consistency of fecal pellet were almost the same as observed in normal control group.Conclusion:From the present study, it is concluded that Trisama has significant intestinal motility-enhancing property in mice which may be useful in gastric problems without affecting the general physiology.

Fermented polyherbal formulation restored ricinoleic acid induced diarrhea in Sprague Dawley rats and exhibitedin vitroantibacterial effect on multiple antibiotics resistant gastric pathogens

“…The recent study had put some light on the anti-motility effect of the polyherbal formulation AP-01. The assessment of in vivo passage of the drug was indirectly done by using a marker (Kaolin) which lightens the color of stool which can be easily distinguished from the normal or wet stool color of rats[33], without affecting the pharmacodynamics. Significant hindrance of gastric motility was observed with AP-01 in comparison to standard drugs where no significant effects were noticed after six to seven hours of administration.…”

“…Therefore, the measured time gap between the feeding of Kaolin and expulsion of fecal matter marked with Kaolin for each animal could serve as a determinant of gastric motility because it could be assessed as the time taken by the Kaolin to get a passage through the gastro-intestinal tract of the rats. Longer latency before the expulsion of Kaolin-marked fecal matter could be suggestive of decreasing gastric motility rates[29][33].…”