Determination of Polyphenols in <i>Lycium barbarum</i> Leaves by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Zhu, Li; Peng, Zumao; Zhang, Xieguang; Yang, Jun; Lai, Xiaofang; Yang, Guowu

doi:10.1080/00032719.2016.1202956

Cited by 10 publications

(6 citation statements)

References 20 publications

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“…This compound has been recognized for its anti-UV capacity; therefore, cultivated leaves might be good sources for anti-radiation food or anti-UV cosmetics. Other polyphenols including quercetin, isoquercitrin, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid, p-coumaric acid, luteolin, kaempferol, and caffeic acid, have been found in the leaves of Goji berries [ 8 ]. All these compounds are of great interest in treating a wide variety of diseases.…”

Section: Introductionmentioning

confidence: 99%

Biologically Active Compounds from Goji (Lycium Barbarum L.) Leaves Aqueous Extracts: Purification and Concentration by Membrane Processes

2020

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Goji (Lycium barbarum L.) leaves and fruits have been described as a valuable source of bioactive compounds with a great potential for the development of health-promoting formulations. The present study aimed to evaluate the potential of a sustainable process for the recovery of phenolic compounds from Goji leaves through a combination of aqueous extraction and membrane-based operations. Water was used as a safe, cheap, and non-hazardous extraction solvent, and parameters of extraction of dried Goji leaves were optimized in order to maximize the yield of polyphenols, total soluble solids (TSS), and total antioxidants simultaneously. The aqueous extract was clarified by ultrafiltration and then processed with three flat-sheet polyethersulphone (PES) membranes with molecular weight cut-off (MWCO) values in the range of 0.3–4.0 kDa, in order to remove sugar compounds from polyphenols and improve the antioxidant activity of the produced fractions. Among the selected membranes, a 1 kDa membrane exhibited the best performance in terms of purification of polyphenols from the clarified aqueous extract. The rejection by this membrane of TSS and total carbohydrates was in the range of 15.8–25.3%, and was decreased by increasing the volume reduction factor (VRF). On the other hand, the retention values for total polyphenols and total antioxidant activity (TAA) were in the range of 73–80%, and were increased by increasing the VRF.

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Section: Introductionmentioning

confidence: 99%

Biologically Active Compounds from Goji (Lycium Barbarum L.) Leaves Aqueous Extracts: Purification and Concentration by Membrane Processes

2020

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“…With diabetic patients, the inclusion of goji fruits in their diet has had demonstrated hypoglycaemic and lipid-lowering effects. Moreover, goji berries can be used in the prevention of neurodegenerative diseases, mainly due to the neuroprotective effect of the contained polysaccharide complex [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Polyphenol Composition and Antioxidant Potential of Instant Gruels Enriched with Lycium barbarum L. Fruit

et al. 2020

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Goji fruit (Lycium barbarum L.) has been identified as a polyphenolic compound plant source of noted richness. It also contains polysaccharides, carotenoids, vitamins and minerals, fatty and organic acids. The purpose of the presented research was to produce innovative instant corn gruels with various dry goji berry contents (1, 3 and 5%), to determine the level of included polyphenolic compounds (including individual free phenolic acids) and to assess the antioxidant properties of these functional-food products. A further objective was to identify the optimum value of one of the most important production parameter, the rotational speed of the extruder’s screw during gruel processing. The undertaken chromatographic analysis (LC-ESI-MS/MS) showed a wide variety of available phenolic acids. In the samples with 5% addition of fruit, eight phenolic acids were detected, whereas in the corn gruel without additives, only five were noted. The antioxidant activity, the content of free phenolic acids and the sum of polyphenols increased with increase of the functional additive. For all goji content, screw speeds of 100 and 120 rpm rather than 80 rpm resulted in higher polyphenol amounts and greater Trolox equivalent antioxidant capacity, as well as higher ability to scavenge DPPH.

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“…For chlorogenic acid ( 23 ), where caffeic acid forms an ester bond with quinic acid, the ester bond fragmented to produce m/z 191.0514 and m/z 179.0460, representing a quinic acid unit and a caffeic acid unit, respectively. Based on these fragmentation patterns, the other selected phenolic acids ( 1 , 7 , 13 , 15 , 22 , 38 , 41, and 45 ) were tentatively characterized ( Wang et al, 2014 ; Yan et al, 2014 ; Lee et al, 2015 ; Zhang et al, 2016 ; Zhu et al, 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Chemical Profiling and Comparison of Sangju Ganmao Tablet and Its Component Herbs Using Two-Dimensional Liquid Chromatography to Explore Compatibility Mechanism of Herbs

Liu

et al. 2018

Front. Pharmacol.

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Sangju Ganmao tablet (SGT), a well-known Chinese patent medicine used to treat cold symptoms, is made from eight herbal medicines. In this study, an off-line hydrophilic interaction × reversed-phase two-dimensional liquid chromatography (HILIC × RP 2D-LC) method was developed to comprehensively separate the chemical constituents of SGT. Through optimization of the experimental conditions, a total of 465 peaks were finally detected in SGT, and the structures of 54 selected compounds were fully identified or tentatively characterized by quadrupole time-of-flight mass spectrometry (qTOF-MS) analysis. The established 2D-LC analysis showed high orthogonality (63.62%) and approximate 11-fold improvement in peak capacity (2399 and 1099, obtained by two calculation methods), in contrast to conventional one-dimensional RPLC separation. The eight component herbs of SGT were also respectively separated by using the 2D-LC system, and we found that a total of 12 peaks detected in SGT were not discovered in any component herbs. These newly generated chemical constituents would benefit better understanding of the compatibility mechanism of the component herbs. The strategy established in this study could be used for systematic chemical comparison of SGT and its component herbs, which contributes to exploration of herbal compatibility mechanism.

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Determination of Polyphenols in Lycium barbarum Leaves by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Cited by 10 publications

References 20 publications

Biologically Active Compounds from Goji (Lycium Barbarum L.) Leaves Aqueous Extracts: Purification and Concentration by Membrane Processes

Biologically Active Compounds from Goji (Lycium Barbarum L.) Leaves Aqueous Extracts: Purification and Concentration by Membrane Processes

Polyphenol Composition and Antioxidant Potential of Instant Gruels Enriched with Lycium barbarum L. Fruit

Chemical Profiling and Comparison of Sangju Ganmao Tablet and Its Component Herbs Using Two-Dimensional Liquid Chromatography to Explore Compatibility Mechanism of Herbs

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