Determination of polyvinyl alcohol in a poly(DL-lactide-co-glycolide) matrix by size exclusion chromatography using evaporative light scattering detection

Dunn, Kimberly D.

doi:10.1016/s0731-7085(00)00525-2

Cited by 6 publications

(2 citation statements)

References 3 publications

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“…Researches on quantitative analysis of PVA have been reported in the literature using ultraviolet spectrophotometric detection [9][10][11][12], infrared spectrophotometric detection [13], static laser scattering detection [14], gel filtration liquid chromatography with refractometry [15], adsorptive stripping voltammetry [16], SEC with evaporative light scattering detector (ELSD) [17] or RID [18], and reversed-phase high-performance liquid chromatography (RP-HPLC) with a chiral column [19]. Most of these methods are used in the textile or paper industry by detecting the absorbance of PVA-iodine-borate complex according to Beer-Lambert law.…”

Section: Introductionmentioning

confidence: 99%

“…Although there are sparse literature works [17][18][19] that aimed at developing a method for pharmaceutical use, these methods suffer from the need for pricey equipment, bad resolution power, band broadening, low sensitivity, and limited application fields. e SEC method in reference [17] has obvious advantages in terms of high sensitivity with LOD of 4∼20 μg/mL and excellent accuracy with recoveries of 96.03∼101.22%. However, expensive ELSD is necessary, resulting in low usability and universality.…”

Section: Introductionmentioning

confidence: 99%

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Development and Validation of a Novel High-Performance Liquid Chromatography (HPLC)/Refractive Index Detector (RID) Method to Determine Residual Polyvinyl Alcohol (PVA) in Microspheres

Yin

Bin

Wang

et al. 2022

Journal of Chemistry

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Polyvinyl alcohol (PVA) is a commonly used emulsifier for the preparation of DL-lactide-co-glycolide (PLGA) and polylactic acid (PLA) microspheres with sustained-release profile. Although water-soluble PVA is normally considered to have low toxicity, monitoring residual level of PVAs is still necessary, especially for the chronic disease treatment medications containing PVA. In this study, a robust and validated method was developed for the determination of PVA using high-performance liquid chromatography (HPLC) and refractive index detector (RID). PVA in sustained-release microspheres containing PLGA, aripiprazole, mannitol, and PVA as active substances was analyzed by size exclusion chromatography (SEC) using acetonitrile/water/trifluoroacetic acid (TFA) (300 : 700 : 1; v/v/v) as mobile phase at a flow rate of 0.5 mL/min. The retention time was 11.2 min and PVA was well separated with other components with good linearity (r2 = 0.9997) in the range of 2.5∼75 μg/mL. The limit of detection (LOD) was 0.51 μg/mL and the limit of quantification (LOQ) was 2.53 μg/mL. The relative standard deviations of intra- and interday precision under different concentrations were not more than 2.1%, and the recoveries were all in the ranges of 95∼105%. The established method has been demonstrated to be accurate, precise, repeatable, specific, and robust and, therefore, suitable for routine analysis of PVA in not only pharmaceutical field but also food and textile industries.

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