Determination of Prooxidant–Antioxidant Balance during Uncomplicated Pregnancy Using a Rapid Assay

Abstract

SummaryBackground: Oxidative stress is thought to be a major contributor to complications during pregnancy, for example preeclampsia. However, reports regarding prooxidant-antioxidant balance in uncomplicated pregnancy are inconsistent. In this study, we aimed to compare the levels of oxidative stress in non-pregnant women with apparently normal pregnant women during the first trimester and at delivery. Methods: An assay for the determination of prooxidantantioxidant balance (PAB) was used in this study, in which the prooxidant burden and the antioxidant capacity were measured simultaneously in a single assay. The levels of oxidative stress were determined in 85 non-pregnant and 64 primigravid pregnant women. Results: Demographic data and biochemical indices did not differ significantly between the groups. Differences between PAB values were significant based on one-way ANOVA analysis (P<0.001). Using a post hoc test, we observed a statistically significant increase in PAB values during the first trimester and last trimester (P<0.001). Conclusions: Normal pregnancy is associated with a change in the measure of redox status, as assessed by the PAB assay.

Assessment of Oxidative and Antioxidant Capacity of Biological Substrates by Chemiluminescence Induced by Fenton Reaction

“…For biological substrates prooxidant-antioxidant balance is important. The works have been performed, in which this balance was estimated in a single experiment [21,22]. To evaluate the balance, the ability of TMB (3,3'.5,5'-tetramethylbenzidine.…”

Determination of Prooxidant and Antioxidant Balance, Clinical Parameters and Nutrient Intakes in Hemodialysis Patients

“…PAB is calibrated using a mixture of hydrogen peroxide and uric acid. The values are expressed in an arbitrary HK unit, which is as hydrogen peroxide percentage in the calibration mixture and demonstrates the oxidative stress index [1,2,17].…”

“…Despite the role of oxidative stress in patients on dialysis determination of PAB yet as a routine clinical laboratory methods are known. Many methods, separately measure the total antioxidant capacity and prooxidant, which is a complex and costly method [1,2]. In this study, the oxidant and antioxidant capacity in an evaluation was measured.…”

“…Oxidative stress can damage cellular components, such as lipids, proteins or DNA [2,5]. There is evidence that suggests the increased state of oxidative stress in uremic patients on hemodialysis (HD) treatment may be related to disturbance in antioxidant mechanisms of free radicals detoxification [3,6,7].…”

“…The importance of oxidative stress in the pathogenesis of many diseases, including inflammatory, chronic and progressive diseases has been recently emphasized [1][2][3][4]. Oxidative stress can damage cellular components, such as lipids, proteins or DNA [2,5].…”