Determination of propranolol concentration in small volume of rat plasma by HPLC with fluorometric detection

Kim, H. K.; Hong, Jisu; Park, M. S.; Kang, Jae Seon; Lee, M. H.

doi:10.1002/bmc.110

Cited by 18 publications

(4 citation statements)

References 28 publications

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“…proprano-lol (Borchard, 1990). Pharmacokinetic studies in rats showed that propranolol half-life may vary between 45 and 90 min (Shand, et al, 1972;Bianchetti, et al, 1980;Kim, et al, 2001). This short half-life may explain propranolol peak effect against nicotine cue-induced reinstatement after 30 min from pre-treatment (corresponding to the 30-min time bin of the experimental session).…”

Section: Discussionmentioning

confidence: 99%

Propranolol transiently inhibits reinstatement of nicotine-seeking behaviour in rats

et al. 2008

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Noradrenergic transmission has been implicated in the affective component of relapse to tobacco smoking. Evidence in human and laboratory animals showed that smoking or nicotine administration may cause changes of the noradrenergic system resulting in hyperactivity in this system after cessation. It has been hypothesised that the anti-adrenergic beta-blocker propranolol may decrease affective activation and arousal observed during drug withdrawal or cue-induced relapse. The aim of the present work was to test the effects of propranolol pre-treatment in a rat model of nicotine cue-induced relapse to nicotine seeking. We also tested the effects of propranolol on food cue-induced reinstatement of food seeking in rats trained on food self-administration. Propranolol transiently inhibited nicotine cue-induced reinstatement. The inhibitory effect of propranolol reached a peak after 30 min from the beginning of the reinstatement session and then it declined until it was completely absent at the end of the 3-h session. This inhibitory effect of propranolol was not observed when the drug was tested versus reinstatement with food cues. The present study suggests a weak effect of propranolol to counteract nicotine cue-induced reinstatement of nicotine seeking. Therefore, these findings do not support a potential use of propranolol for prevention of smoking relapse.

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Section: Discussionmentioning

confidence: 99%

Propranolol transiently inhibits reinstatement of nicotine-seeking behaviour in rats

et al. 2008

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“…This procedure was adapted from [31][32][33] for propranolol and from [34] for furosemide. Briefly, the separation was performed on a C18 analytical column (Macherey-Nagel LiChrospher RP 18e; 5 µm; 125 × 4 mm) eluted with a mobile phase composed of acetonitrile added with 14 mM triethylamine in water buffered with orthophosphoric acid, pH 2.5 (30/70; v/v) at a flow rate of 1.0 mL.min −1 and with a column temperature of 40°C.…”

Section: Quantification Of Furosemide and Propranololmentioning

confidence: 99%

Intestinal absorption of S-nitrosothiols: Permeability and transport mechanisms

Bonetti

Zhou

Parent

et al. 2018

Biochemical Pharmacology

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S-Nitrosothiols, a class of NO donors, demonstrate potential benefits for cardiovascular diseases. Drugs for such chronic diseases require long term administration preferentially through the oral route. However, the absorption of S-nitrosothiols by the intestine, which is the first limiting barrier for their vascular bioavailability, is rarely evaluated. Using an in vitro model of intestinal barrier, based on human cells, the present work aimed at elucidating the mechanisms of intestinal transport (passive or active, paracellular or transcellular pathway) and at predicting the absorption site of three S-nitrosothiols: S-nitrosoglutathione (GSNO), S-nitroso-N-acetyl-l-cysteine (NACNO) and S-nitroso-N-acetyl-d-penicillamine (SNAP). These S-nitrosothiols include different skeletons carrying the nitroso group, which confer different physico-chemical characteristics and biological activities (antioxidant and anti-inflammatory). According to the values of apparent permeability coefficient, the three S-nitrosothiols belong to the medium class of permeability. The evaluation of the bidirectional apparent permeability demonstrated a passive diffusion of the three S-nitrosothiols. GSNO and NACNO preferentially cross the intestinal barrier though the transcellular pathway, while SNAP followed both the trans- and paracellular pathways. Finally, the permeability of NACNO was favoured at pH 6.4, which is close to the pH of the jejunal part of the intestine. Through this study, we determined the absorption mechanisms of S-nitrosothiols and postulated that they can be administrated through the oral route.

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“…From the chemical point of view, propranolol is an organic compound described as 1-[(1-methylethyl) amino]-3-(1-naphthalenyloxy). In the literature various analytical propranolol determination methods have been reported, among them were spectrophotometry [4,5], spectrofluorimetry [6,7], high performance liquid chromatography (HPLC) [8][9][10], and capillary electrophoresis [11,12]. Another method that is commonly used for propranolol determination is voltammetry.…”

Section: Introductionmentioning

confidence: 99%

A Novel Voltametric Measurements of Beta Blocker Drug Propranolol on Glassy Carbon Electrode Modified with Carbon Black Nanoparticles

et al. 2021

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A new voltametric method for highly sensitive propranolol (PROP) determination was developed. A glassy carbon electrode modified with a hybrid material made of carbon black (CB) and Nafion was used as the working electrode. The preconcentration potential and time were optimized (550 mV and 15 s), as well as the supporting electrolyte (0.1 mol L−1 H2SO4). For 15 s preconcentration time, linearity was achieved in the range 0.5–3.5 μmol L−1 and for 120 s in 0.02–0.14 μmol L−1. Based on the conducted calibration (120 s preconcentration time) limit of detection (LOD) was calculated and was equal to 7 nmol L−1. To verify the usefulness of the developed method, propranolol determination was carried out in real samples (tablets and freeze-dried urine). Recoveries were calculated and were in the range 92–102%, suggesting that the method might be considered as accurate. The repeatability of the signal expressed as relative standard deviation (RSD) was equal to 1.5% (n = 9, PROP concentration 2.5 µmol L−1). The obtained results proved that the developed method for propranolol determination might be successfully applied in routine laboratory practice.

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Determination of propranolol concentration in small volume of rat plasma by HPLC with fluorometric detection

Cited by 18 publications

References 28 publications

Propranolol transiently inhibits reinstatement of nicotine-seeking behaviour in rats

Propranolol transiently inhibits reinstatement of nicotine-seeking behaviour in rats

Intestinal absorption of S-nitrosothiols: Permeability and transport mechanisms

A Novel Voltametric Measurements of Beta Blocker Drug Propranolol on Glassy Carbon Electrode Modified with Carbon Black Nanoparticles

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