Determination of Protein in Lupin Using Liquid Chromatography Coupled with Organic Carbon Detector and Organic Nitrogen Detector (LC-OCD-OND): Validation of a New Method

Chandra-Hioe, Maria V.; Antony, Alice; Arcot, Jayashree

doi:10.17756/jfcn.2018-061

Cited by 1 publication

(1 citation statement)

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“…Media composition with low nitrogen level produce (1.42 g/L) as compared to higher nitrogen concentrations with biosurfactant yield of 0.94 g/L (Prieto et al 2008 ). The Kjeldahl or Dumas methods used for the evaluation of the crude protein in foods determine the total organic nitrogen of foods (Chandra-Hioe et al 2018 ). The nitrogen content determination is crucial for the analysis of crude protein content.…”

Section: Discussionmentioning

confidence: 99%

Low-cost production and application of lipopeptide for bioremediation and plant growth by Bacillus subtilis SNW3

et al. 2021

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At present time, every nation is absolutely concern about increasing agricultural production and bioremediation of petroleum-contaminated soil. Hence, with this intention in the current study potent natural surfactants characterized as lipopeptides were evaluated for low-cost production by Bacillus subtilis SNW3, previously isolated from the Fimkessar oil field, Chakwal Pakistan. The significant results were obtained by using substrates in combination (white beans powder (6% w/v) + waste frying oil (1.5% w/v) and (0.1% w/v) urea) with lipopeptides yield of about 1.17 g/L contributing 99% reduction in cost required for medium preparation. To the best of our knowledge, no single report is presently describing lipopeptide production by Bacillus subtilis using white beans powder as a culture medium. Additionally, produced lipopeptides display great physicochemical properties of surface tension reduction value (SFT = 28.8 mN/m), significant oil displacement activity (ODA = 4.9 cm), excessive emulsification ability (E24 = 69.8%), and attains critical micelle concentration (CMC) value at 0.58 mg/mL. Furthermore, biosurfactants produced exhibit excellent stability over an extensive range of pH (1–11), salinity (1–8%), temperature (20–121°C), and even after autoclaving. Subsequently, produced lipopeptides are proved suitable for bioremediation of crude oil (86%) and as potent plant growth-promoting agent that significantly (P < 0.05) increase seed germination and plant growth promotion of chili pepper, lettuce, tomato, and pea maximum at a concentration of (0.7 g/100 mL), showed as a potential agent for agriculture and bioremediation processes by lowering economic and environmental stress. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%