Low-cost production and application of lipopeptide for bioremediation and plant growth by Bacillus subtilis SNW3

Abstract

At present time, every nation is absolutely concern about increasing agricultural production and bioremediation of petroleum-contaminated soil. Hence, with this intention in the current study potent natural surfactants characterized as lipopeptides were evaluated for low-cost production by Bacillus subtilis SNW3, previously isolated from the Fimkessar oil field, Chakwal Pakistan. The significant results were obtained by using substrates in combination (white beans powder (6% w/v) + waste frying oil (1.5% w/v) and (0.1% w/v) urea) with lipopeptides yield of about 1.17 g/L contributing 99% reduction in cost required for medium preparation. To the best of our knowledge, no single report is presently describing lipopeptide production by Bacillus subtilis using white beans powder as a culture medium. Additionally, produced lipopeptides display great physicochemical properties of surface tension reduction value (SFT = 28.8 mN/m), significant oil displacement activity (ODA = 4.9 cm), excessive emulsification ability (E24 = 69.8%), and attains critical micelle concentration (CMC) value at 0.58 mg/mL. Furthermore, biosurfactants produced exhibit excellent stability over an extensive range of pH (1–11), salinity (1–8%), temperature (20–121°C), and even after autoclaving. Subsequently, produced lipopeptides are proved suitable for bioremediation of crude oil (86%) and as potent plant growth-promoting agent that significantly (P < 0.05) increase seed germination and plant growth promotion of chili pepper, lettuce, tomato, and pea maximum at a concentration of (0.7 g/100 mL), showed as a potential agent for agriculture and bioremediation processes by lowering economic and environmental stress. Graphical Abstract

A new bacterial-derived biosurfactant for biotechnological applications in the oil industry: production, optimization, biosurfactant functional and physicochemical characterization

“…These results are of growing interest as the prospective application of BioS in numerous industrial processes necessitates thermal stabilities. Previous research works exhibited similar findings(Chen et al 2017;Ghazala et al 2017;Kiran et al 2017;Martins et al 2018;Feng et al 2019;Purwasena et al 2019;Zouari et al 2019;Jimenez et al 2021;Mnif et al 2021aMnif et al , 2021bUmar et al 2021;Wu et al 2022).…”

“…Similar results are observed for emulsifying activity with higher attenuation than foaming power. These results go in favor of broadening its purview of use in different areas, especially for the enhanced oil recovery and biological remediation of sea water contamination (Chen et al 2017;Ghazala et al 2017;Kiran et al 2017;Martins et al 2018;Feng et al 2019;Purwasena et al 2019;Zouari et al 2019;Jimenez et al 2021;Mnif et al 2021aMnif et al , 2021bUmar et al 2021;Wu et al 2022).…”

WITHDRAWN: Global metabolic changes by Bacillus cyclic lipopeptide extracts on stress responses of para rubber leaf

“…While, fengycin A and iturin A presented the potency of plant photosynthesis involving in plant growth promotion (Wang et al 2020). In addition, Bacillus CLPs were reported the ability to increase seed germination and plant growth promotion in chili pepper, lettuce, tomato, and pea (Umar et al 2021).…”

Comparative study of coating agents for prevention of fine-dust-induced light transmittance reduction in greenhouse covering materials

“…Polymer bio-surfactants are polymer materials (Jung et al, 2014), and if a nanostructure forms on the surface of the polymer, a superhydrophobic surface can be made without using expensive equipment (Yoon et al, 2019). Lipoptide and glycolipids have the hydrophobicity of biosurfactants (Jung et al, 2009), and they can be produced from low-cost materials (Mnif et al, 2016;Umar et al, 2021). Teflon coating agents are suitable for creating the highest hydrophobic surface; however, they have been steadily regarded as a legal problem with using perfluorinated composites, which are the core inhibitors of Teflon (Kraugud et al, 2011).…”