Determination of ptaquiloside in cow's milk by HPLC-UV

Ribeiro, Débora da Silva Freitas; Keller, Kelly Moura; Melo, Marília Martins; Soto-Blanco, Benito

doi:10.5433/1679-0359.2019v40n4p1715

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…The health effect of bracken fern poisoning varies depending on the species of animal, the ingested dose, and the duration of consumption (5). Among domestic animals, bracken fern poisoning primarily affects cattle and, less frequently, sheep.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the occurrence and cause of bloody urine in cattle and management practices in Cheliya, Jibat, and Dire Inchini districts of the West Shewa Zone, Oromia, Ethiopia

Borena,

Sarba,

Eteya

et al. 2024

Preprint

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Background A study was conducted to assess the causes of bloody urine and management practices in cattle from the Cheliya, Jibat, and Dire Inchini districts of the West Shoa Zone, Oromia, Ethiopia. Materials and methods Two kebeles from each of the three districts were selected based on bloody urine case reports, and a total of 215 cattle-owning households were selected for the questionnaire survey. Field observations were performed to screen for possible causes of bloody urine in the study areas. Six animals with symptoms of bloody urine were clinically examined, followed by postmortem and histopathological examinations. Results The questionnaire survey results showed that 72.1% (n = 155) of the households had at least one bloody urine case in their cattle herd. Among the three districts, more bloody urine cases were reported in the Cheliya district (95.0%), compared to 67.4% in Jibat and 53.5% in Dire Inchini. About 33.0% of the households reported the death of at least one cattle due to bloody urine; 58.8% of which were from the households in Cheliya, while 40.8% in Jibat, and 4.7% in Dire Inchini district. The occurrence of bloody urine was related to the dry season, feed shortage, access to bracken fern-infested pasture, and a known history of eating bracken fern. There was a high infestation rate of bracken fern (Pteridium aquilinum) in the grazing areas. All six suspected cattle exhibited chronic wasting and hematuria. Centrifugation of the urine samples revealed hematuria. Postmortem examination revealed petechial hemorrhage, papillomatous formations, thickened areas of mucosa, and white/red nodules on the urinary bladder. Conclusions Severe infestation of the bracken fern in the study areas, chronic hematuria, and wasting and postmortem findings in the bladder were all suggestive of bracken fern poisoning. Therefore, an immediate and integrated approach should be implemented to prevent cattle from accessing infested areas, remove this plant from grazing areas, and replace it with appropriate forage.

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Section: Introductionmentioning

confidence: 99%

Assessment of the occurrence and cause of bloody urine in cattle and management practices in Cheliya, Jibat, and Dire Inchini districts of the West Shewa Zone, Oromia, Ethiopia

Borena,

Sarba,

Eteya

et al. 2024

Preprint

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“…Analysis of PTA in groundwater is challenging due to the concentrations typically at ng L −1 scale and due to its unstable nature. The analytical methods for quantification of PTA and PtB are based on HPLC-UV [25,41,42], LC-MS [43,44], LC-MS/MS [35,45] and GC-MS [46]. Available analytical methods for PTA analysis in water including pre-concentration are UPLC-MS/MS method with LOD of 0.008 µg L −1 [35] and LC-MS/MS method with LOD 0.01 µg L −1 [28,41].…”

Section: Introductionmentioning

confidence: 99%

A Novel Method for Determination of the Natural Toxin Ptaquiloside in Ground and Drinking Water

Skrbic

Pedersen²,

Christensen³

et al. 2020

Water

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Ptaquiloside (PTA) is a carcinogenic compound naturally occurring in bracken ferns (Pteridium aquilinum). It is highly water soluble and prone to leaching from topsoil to surface and groundwaters. Due to possible human exposure via drinking water, PTA is considered as an emerging contaminant. We present a sensitive and robust method for analysis of PTA and its degradation product pterosin B (PtB) in groundwater. The method comprises two steps: sample preservation at the field site followed by sample pre-concentration in the laboratory. The preservation step was developed by applying a Plackett–Burman experimental design testing the following variables: water type, pH, filtering, bottle type, storage temperature, transportation conditions and test time. The best sample preservation was obtained by using amber glass bottles, unfiltered solutions buffered at pH 6, transported without ice, stored at 4 °C and analysed within 48 h. The recovery was 94% to 100%. The sample purification step had a pre-concentration factor of 250, and the recovery percentages of the entire method were 85 ± 2 (PTA) and 91 ± 3 (PtB). The limits of detection (LOD) of the full method were 0.001 µg L−1 and 0.0001 µg L−1 for PTA and PtB, respectively. The method enables sensitive monitoring of PTA and PtB in groundwater. Carcinogenic PTA was detected in one groundwater well (0.35 µg L−1).

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“…In Brazil, only the last three syndromes have been reported in cattle [3][4][5]. In addition to poisoning the cattle, the consumption of the plant by these animals presents a potential risk for humans, since residues of ptaquiloside can be found in milk [6][7][8] and meat [9,10]. Several chemical compounds have been identified in Pteridium ferns, the main one being a norsesquiterpenoid glycoside called ptaquiloside, which has been implicated in cattle poisoning and human cancer [3,4].…”

Section: Introductionmentioning

confidence: 99%

Ptaquiloside and Pterosin B Levels in Mature Green Fronds and Sprouts of Pteridium arachnoideum

Ribeiro¹,

Keller

Soto-Blanco

2020

Toxins

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Pteridium arachnoideum, a fern of the Pteridium aquilinum species complex found in South America, is responsible for several different syndromes of poisoning. Cases of bovine enzootic hematuria and upper alimentary squamous cell carcinoma are both frequent occurrences in Brazil, whereas only bovine enzootic hematuria is noted with any frequency around the world. The reason for the high frequency of upper alimentary squamous cell carcinoma in Brazil is not currently known. One possible explanation may be the higher levels of ptaquiloside and pterosin B in Brazilian Pteridium than those present in the plant in other countries. However, these levels have not yet been determined in P. arachnoideum. Thus, the present study aimed to measure and compare ptaquiloside and pterosin B levels in mature green fronds and sprouts of P. arachnoideum collected from different locations in Brazil. Samples of P. arachnoideum were collected from the states of Minas Gerais and Rio Grande do Sul. A total of 28 mature leaf samples and 23 sprout samples were used. The mean concentrations of ptaquiloside and pterosin B present in the mature green fronds of P. arachnoideum ranged from 2.49 to 2.75 mg/g and 0.68 to 0.88 mg/g, respectively; in P. arachnoideum sprouts, mean concentrations of ptaquiloside and pterosin B ranged from 12.47 to 18.81 mg/g, and 4.03 to 10.42 mg/g for ptaquiloside and pterosin B, respectively. Thus, ptaquiloside and pterosin B levels in P. arachnoideum samples collected in Brazil were higher in sprouts than in mature green fronds, as observed in other countries. However, there was no variation in ptaquiloside levels among plants collected from different cities in Brazil. The high frequency of upper alimentary squamous cell carcinoma in Brazilian cattle may not be attributed to greater levels of ptaquiloside and pterosin B in P. arachnoideum than in other Pteridium species in other countries.

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Determination of ptaquiloside in cow's milk by HPLC-UV

Cited by 3 publications

References 11 publications

Assessment of the occurrence and cause of bloody urine in cattle and management practices in Cheliya, Jibat, and Dire Inchini districts of the West Shewa Zone, Oromia, Ethiopia

Assessment of the occurrence and cause of bloody urine in cattle and management practices in Cheliya, Jibat, and Dire Inchini districts of the West Shewa Zone, Oromia, Ethiopia

A Novel Method for Determination of the Natural Toxin Ptaquiloside in Ground and Drinking Water

Ptaquiloside and Pterosin B Levels in Mature Green Fronds and Sprouts of Pteridium arachnoideum

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