Determination of Quality Characteristics of Kundhi Buffalo Bull Semen

Kaka, Asmatullah; Memon, Akeel Ahmed; Khatri, P.; Kumbhar, H. K.; Kalhoro, Dildar Hussain; Tariq, Mohammad Masood; Pirzado, Shoaib Ahmed; Tunio, Ahmed

doi:10.6000/1927-5129.2016.12.61

Cited by 6 publications

(8 citation statements)

References 7 publications

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“…The color, consistency and pH of the semen in this study tended to be stable. Buffalo semen in this study had a pH of 6.4 which was in accordance to the reports of Kaka et al (2016) that the pH variation of buffalo semen is around 6.1 to 6.8. Microscopic characteristics of fresh semen obtained in this study include mass movement (+ +), sperm motility (70.35 ± 0.89%), sperm viability (83.21± 0.89%), plasma membrane integrity (83.78 ± 0.53%), sperm abnormalities (15.34 ± 0.53%) and sperm concentrations (1.154 ±60.76 million cells mL -1 ).…”

Section: Resultssupporting

confidence: 92%

“…The characteristics of fresh semen have had a normal range and are suitable for freezing (Table 1) . The volume of swamp buffalo semen ranges from 1.9 to 3.4 mL (Koonjaenak et al, 2007) and the pH variation of buffalo semen is between 6.1 and 6.8 due to the metabolic activity of sperm which produce lactic acid (Kaka et al, 2016). The same characteristics are also reported in swamp buffalo in Thailand (Koonjaenak et al, 2007).…”

Section: Discussionmentioning

confidence: 52%

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Comparison of different cryoprotective agents on swamp buffalo semen cryopreservation

Herbowo

Arifiantini

Karja

et al. 2020

J. Indonesian Trop. Anim. Agric.

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Present study aimed to determine the effectivenessof dimethylformamide (DMF)as cryoprotective agent (CPA) by determining its optimum concentration inTris Eggyolk (TEY) extender on the quality of post-thaw swamp buffalo semen. Only ejaculates having > 60% sperm motility were divided into eight s tubes. Four tubes were diluted in TEY with 4, 5, 6, 7% of glycerol and four other tubes were diluted in TEY with 4, 5, 6, 7% of DMF. Diluted semen then packed into ministraw (0.25 mL), equilibrated at 4°C for 4 hours and freezed at 8 cm above liquid nitrogen vapour for 10 minutes and then stored at liquid nitrogencontainer. After 24 hours of storage, straws were thawed at 37°C for 30 seconds. The result showed that sperm motility after equilibration tend to be the same at all four glycerol concentrations, whereas the decrease in motility was highest in extenders with a DMF concentration of 7% (P <0.05). Post-thaw sperm motility and recovery rate in TEY extender containing 5% DMF or 7% glycerol were highest (P<0.05) than other glycerol or DMF concentration. Sperm viability and membrane integrity did not differ among TEY containing 6% or 7% glycerol, both were higher (P<0.05) than 4% or 5% glycerol. Viability and membrane integrity of sperm in 5% DMF was superior (P<0.05) to 4%, 6% or 7%. This research concluded that 5% DMF or 7% glycerol was considered to be the most suitable CPAs for swamp buffalo semen cryopreservation.

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Section: Resultssupporting

confidence: 92%

Section: Discussionmentioning

confidence: 52%

Comparison of different cryoprotective agents on swamp buffalo semen cryopreservation

Herbowo

Arifiantini

Karja

et al. 2020

J. Indonesian Trop. Anim. Agric.

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“…The best recovery rates of buffalo sperm were obtained on andromed® diluents followed by tris egg-yolk compared to triladyl® diluents at sperm concentrations of 20 x 10 6 sperm/mL. The results of this study also support the findings of previous research Bhakat et al (2011), Ghodasara et al (2016) and Kaka et al (2016).…”

Section: The Frozen Semen Qualitysupporting

confidence: 90%

The Quality of Buffalo Sperm Following Preservation Using Different Diluents and Sperm Concentrations

et al. 2022

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Artificial Insemination (AI) success depends on the quality of the frozen semen. The quality of the frozen semen of swamp buffalo in Indonesia is still low. The study was conducted to determine the quality of buffalo sperm following freezing using three different diluents and three different doses. The study used buffalo semen from the Tuah Sakato Artificial Insemination Center, Payakumbuh (n = 3). The semen collecting was carried out once a week for 10 weeks (replication). The research method used was 3x3 factorial randomized block design. The first factor was diluent (Triladyl®, Andromed® and Tris egg- yolk) and the second factor was the dose of spermatozoa (10 and 15 and 20 x 106 sperm/ml). Data were analyzed using variant analysis, while the differences between treatments were tested by Duncan Multiple Range Test. The results showed that the plasma membrane integrity of buffalo sperm was found in Andromed® diluent, while tris egg-yolk diluent gave better motility, viability, plasma membrane integrity and recovery rate at a sperm concentration of 20 x106 sperm/mL compared to triladyl® diluent and a sperm concentration of 10 and 15 x106 sperm/mL. It was concluded that andromed® diluent and tris egg-yolk gave better motility, viability, plasma membrane integrity and recovery rate at a sperm concentration of 20 x106 sperm/mL compared to triladyl® diluent and a sperm concentration of 10 and 15 x106 sperm/mL. of Buffalo of sperm abnormalities not sicnificantly by the type of diluent but are influenced by sperm concentration.

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“…The collected semen can be continued for the cryopreservation process because the fresh semen has motility above 70%, viability above 80%, and abnormalities below 5%. According to Kaka et al (2016), the progressive motility of sperm in fresh sperm is a viable indicator for the freezing process, and the percentage of motility was above 60% (MacKenna et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Supplementation of Pandanus conoideus Oil in Cryopreservation Diluents for Maintaining the Semen Quality of Ongole Grade Bull

Nurcholis¹,

Furqon

Arifiantini

et al. 2021

Trop. Anim. Sci. J.

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Antioxidants such as tocopherol, ß-carotene, and polyunsaturated fatty acids (PUFA) from red fruit oil of Papua may be used to protect frozen semen. The study is aimed to test the effect of red fruit oil supplementation on motility, viability, and recovery rate of frozen sperm of Ongole-grade bulls. Semen was collected twice a month from eight 4-5-year-old male Ongole grade using an artificial vagina, followed by macro- and microscopical evaluations. Collected semen was divided into four tubes and diluted with tris egg yolk diluents (TEY) as a control, TEY supplemented with 0.5% red fruit oil (RFO) (TEY RFO0.5), TEY supplemented with 1% RFO (TEY RFO1.0), and TEY supplemented with 1.5% RFO (TEY RFO1.5). The diluted semen was then packed into the straw and equilibrated for 2, 4, and 6 h prior to frozen on liquid nitrogen vapor for 10 minutes. The observed variables in this study were sperm motility, sperm viability, and morphology after equilibration, after thawing, and recovery rate. The experimental design is a completely randomized factorial design. The data were analyzed using ANOVA and were further tested using Duncan multiple range test. The results showed that the sperm motility of fresh semen was 81.10±1.42%. The percentage of sperm motility in TEY RFO1.5 treatment at 6 h equilibration was 60.00±1.06%, significantly higher compared to TEY RFO1.0 and TEY RFO0.5. The percentage of post-thawing sperm motility in TEY RFO1.5 treatment was 62.40±1.09%. The best post-thawing sperm viability in TEY RFO1.5 was 80.70±1.20%, significantly increase from the treatment of TEY RFO1.0 and TEY RFO0.5. The recovery rate (RR) for TEY RFO1.5 treatment had the best percentage at 76.94%. In conclusion, RFO supplementation in semen diluents for 2 h of equilibration resulted in the best motility and viability at 0 h of post thawing observation.

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Determination of Quality Characteristics of Kundhi Buffalo Bull Semen

Cited by 6 publications

References 7 publications

Comparison of different cryoprotective agents on swamp buffalo semen cryopreservation

Comparison of different cryoprotective agents on swamp buffalo semen cryopreservation

The Quality of Buffalo Sperm Following Preservation Using Different Diluents and Sperm Concentrations

Supplementation of Pandanus conoideus Oil in Cryopreservation Diluents for Maintaining the Semen Quality of Ongole Grade Bull

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