Supplementation of Pandanus conoideus Oil in Cryopreservation Diluents for Maintaining the Semen Quality of Ongole Grade Bull

Abstract

Antioxidants such as tocopherol, ß-carotene, and polyunsaturated fatty acids (PUFA) from red fruit oil of Papua may be used to protect frozen semen. The study is aimed to test the effect of red fruit oil supplementation on motility, viability, and recovery rate of frozen sperm of Ongole-grade bulls. Semen was collected twice a month from eight 4-5-year-old male Ongole grade using an artificial vagina, followed by macro- and microscopical evaluations. Collected semen was divided into four tubes and diluted with tris egg yolk diluents (TEY) as a control, TEY supplemented with 0.5% red fruit oil (RFO) (TEY RFO0.5), TEY supplemented with 1% RFO (TEY RFO1.0), and TEY supplemented with 1.5% RFO (TEY RFO1.5). The diluted semen was then packed into the straw and equilibrated for 2, 4, and 6 h prior to frozen on liquid nitrogen vapor for 10 minutes. The observed variables in this study were sperm motility, sperm viability, and morphology after equilibration, after thawing, and recovery rate. The experimental design is a completely randomized factorial design. The data were analyzed using ANOVA and were further tested using Duncan multiple range test. The results showed that the sperm motility of fresh semen was 81.10±1.42%. The percentage of sperm motility in TEY RFO1.5 treatment at 6 h equilibration was 60.00±1.06%, significantly higher compared to TEY RFO1.0 and TEY RFO0.5. The percentage of post-thawing sperm motility in TEY RFO1.5 treatment was 62.40±1.09%. The best post-thawing sperm viability in TEY RFO1.5 was 80.70±1.20%, significantly increase from the treatment of TEY RFO1.0 and TEY RFO0.5. The recovery rate (RR) for TEY RFO1.5 treatment had the best percentage at 76.94%. In conclusion, RFO supplementation in semen diluents for 2 h of equilibration resulted in the best motility and viability at 0 h of post thawing observation.

Effect of Guava Filtrate Supplementation in Tris and Citrate-Based Extenders on Spermatozoa Quality of Brangus Bull after Sex-rest

“…Progressive motility of spermatozoa is one of important factors to support the fertilization. In this study, the progressive motility of spermatozoa ranges from 60% to 70%, in accordance with reported by Widiarta et al (2019), 69%, Pause et al (2022), maximum 80%, and lower than reported by Nurcholis et al (2021), 81,1 ± 1.42%. The percentage of live spermatozoa were from 60% to 85%, slightly lower than reported by Diansyah et al (2022), 95.73 ± 2.15.…”

Protection of Myrmecodia Extract in Tris Diluent on PO Bull Sperm Quality During Freezing

“…Semen pH is usually correlated with sperm motility, reinforced by Dhumal et al (2021) that semen pH is closely related to sperm motility. The motility and viability of fresh semen sperm typically have normal values above 70% and 80% (Nurcholis et al, 2021). The quality of fresh semen in this study complies with Indonesian national standards (SNI 4869-1:2021).…”

“…The fresh semen volume of PO cattle is an average of 4.7 ml -5.4 ml. Nurcholis et al (2021) volume semen sapi PO pada umur 3 tahun rata-rata 4.8 ml. Penelitian lain voleme semen sapi PO mencapai 6.1 ml (Zamuna et al, 2016).…”

“…According to Ros Santaella and Pintus (2021), using natural plant extracts as additional diluents can protect sperm during cooling. Several extracts have high antioxidant content, such as green tea extract (Prastiya et al, 2023), soybean extract (Ratnawati et al, 2023), Lycopene (Bintara et al, 2023), red fruit oil (Nurcholis et al, 2021), Eurycoma longifolia extract (Baiee et al, 2018). Myrmecodia contains secondary metabolite compounds in the form of flavonoids and tannins which can inhibit dangerous cell proliferation through its antioxidant activity (Imaniar et al, 2022).…”

Integrity of Sperm Cell Bali Bull Preserved at 5°C Using a Diluent Formulation of Extracted Red Fruit and Coconut Water

“…Finally, the structure will be logical, concise, and grammatically accurate. Red fruit juice boasts α-tocopherol, βcarotene, oleic acid, linoleic acid, linolenic acid, vitamin C, calcium, phosphorus, and iron (Tethool et al, 2021;Nurcholisa et al, 2021). Tabatabaei et al (2011) suggest that antioxidants, including tocopherol, β-carotene, and ascorbic acid, can protect spermatozoa cells from cellular morphological damage, which can lead to abnormalities in these cells by preventing the negative effects of free radicals.…”