Effect of Guava Filtrate Supplementation in Tris and Citrate-Based Extenders on Spermatozoa Quality of Brangus Bull after Sex-rest

Abstract

The objective of this study was to determines the effect of guava fruit filtrate (GF) supplementation in Tris and Citrate-based extenders on spermatozoa quality of Brangus bull after sex-break. Semen samples (twelve ejaculates) were diluted in Tris-egg yolk (TEY) without GF (control group, T1), TEY with 10% GF (T2), Citrate-egg yolk (CEY) with 10% GF (T3), Citrate with 10% egg yolk and 10% GF (T4), then chill-storage. The variables quality evaluated include spermatozoa progressive motility, visibility, and abnormality. Spermatozoa quality was observed every 24 hours until the spermatozoa motility reached at least 40%. The results showed that progressive motility and viability on the fourth day storage in extender Citrate-egg yolk (CEY) with 10% egg yolk and 10% GF were 50.4 ± 1.6% and 57.1 ± 1.6%, significantly higher (P<0.05) than control, TEY with 10% GF and Citrate with 10% egg yolk. The spermatozoa abnormality was 12.9 ± 0.3%, the lowest in extender Citrate with 10% egg yolk and 10% GF on the fourth-day storage. In conclusion, supplementation of GF to extender improves the spermatozoa quality of Brangus bull after sex activity break for a long time. Moreover, the best quality of spermatozoa is found in supplementing 10% GF to Citrate with 10% egg yolk extender.

Addition of Sugarcane Juice to Tris Egg Yolk Buffer Diluent Improves the Quality of the Stored Diluted Semen of Kacang Goats at 5°C

“…Sugarcane juice combined with egg yolk was very good at maintaining abnormal spermatozoa (Mahfud et al, 2019). The benefits of egg yolk were found in lipoprotein and lecithin, which played a role in maintaining and protecting the intensity of the lipoprotein sheath and spermatozoa cells from decreasing temperature and stabilizing the plasma membrane (Sumadiasa et al, 2022).…”

“…The smell of collected semen in this study was typical, which indicated normal semen with an absence of contamination. Sumadiasa et al (2022) stated that normal semen has a distinctive fishy odour accompanied by the smell of the animal. However, a foul odor can occur when the semen contains pus caused by an infection of male reflux organs (Lopes, 2002).…”

“…The control diluent (T0) was a Tris egg yolk diluent (Evans and Maxwell, 1989;Sumadiasa et al, 2023). Treatment diluents were T1 = T0 + 10% sugarcane, T2 = T0 + 15% sugarcane, and T3 = T0 + 20% sugarcane.…”