Determination of quantitative retention-activity relationships between pharmacokinetic parameters and biological effectiveness fingerprints of Salvia miltiorrhiza constituents using biopartitioning and microemulsion high-performance liquid chromatography

Gao, Haoshi; Huang, Hongzhang; Zheng, Aiping; Yu, Nuojun; Li, Ning

doi:10.1016/j.jchromb.2017.09.018

Cited by 9 publications

(9 citation statements)

References 33 publications

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“…A positive coefficient indicates that it is positively correlated with the enzyme activity, while a negative number indicates that it is negatively correlated with the enzyme activity. The IC 50 for XO activity increased with increasing peak areas of 3,4,7,10,11,16,17,18,20,21,23,24,25,26,27,28, and 30, indicating that this component group was positively correlated with XO inhibitory activity and that peaks 1,2,5,6,8,9,12,13,14,15,19,22,31,32, and 33 were negatively correlated with their inhibitory activities ( Figure 6). Variable importance in projection (VIP) scores is important indicators that reflect the ability of independent variables to explain dependent variables.…”

Section: Plsr Analysis Resultsmentioning

confidence: 97%

“…These methods enable rapid analysis, complement their respective advantages, and can be used to comprehensively evaluate the quality of L. lucidum. GRA showed that the main components responsible for the XO inhibitory activity of L. lucidum might be characteristic peaks 1,2,4,5,6,7,8,9,11,12,14,15,16,17,20,21,23,25,27,28,29,31,32, and 33. PLS analysis showed that peaks 1, 2, 4, 5, 6, 7, 14, 17, 22, 24, 25, 28, 31, F I G U R E 6 Effect coefficient of each chromatographic peak on the xanthine oxidase inhibitory activity F I G U R E 7 VIP contribution of each characteristic peak to the antioxidant activity of Ligustrum lucidum and 33 had significant effects on the XO inhibitory activity.…”

Section: Plsr Analysis Resultsmentioning

confidence: 99%

“…The gray relational grade is shown in 4,25,26,27,28,29, and 30 was greater than 0.8, indicating that they had a relatively significant effect on the XO activity. Components 1,2,3,5,6,7,8,9,10,11,12,14,15,16,17,18,19,20,21,22,23,24,31,32, and 33 all had correlation degrees greater than 0.7, indicating a significant effect on the XO activity. Combined with the data in the table, it can be seen that the inhibitory activities of characteristic peaks 1,2,4,5,6,7,8,9,11,12,14,15,16,17,20,21,23,25,27,28,29,…”

Section: F I G U R E 3 Cluster Analysis Of the Ligustrum Lucidum Extrmentioning

confidence: 98%

“…To study the basis for the hyaluronic acid‐lowering activity of compounds in L. lucidum , in this study, the activity of XO in vitro was determined by high‐throughput screening, and the HPLC fingerprint of L. lucidum was established . The correlations between characteristic fingerprint components and pharmacodynamic index (IC 50 ) values were analyzed by cluster analysis, principal component analysis (PCA), gray correlation analysis, and partial least squares regression (PLSR) analysis .…”

Section: Introductionmentioning

confidence: 99%

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Study on the spectrum‐effect relationship of the xanthine oxidase inhibitory activity of Ligustrum lucidum

Gao

Chen

Zhou

2019

J of Separation Science

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To evaluate the xanthine oxidase inhibitory activity of the chemical constituents of Ligustrum lucidum in vitro, the spectrum‐effect relationship was investigated. The high‐performance liquid chromatography fingerprint was established by ultraviolet spectrophotometry, and the xanthine oxidase inhibitory activity was tested in vitro by a high‐throughput screening method. Cluster analysis, principal component analysis, gray correlation analysis, and partial least squares regression were used to explore the spectrum‐effect relationships. Sixty batches of Ligustrum lucidum were collected from 16 provinces for testing. The results revealed differences among the batches of medicinal materials, and the similarity score was between 0.635 and 0.968. Thirty‐three characteristic peaks (1–33) were calibrated by fingerprint evaluation software for traditional Chinese medicine. The spectrum‐effect relationship study further revealed that the contents of peaks 1, 2, 4, 5, 6, 7, 14, 17, 25, 28, 31, and 33, which are potentially critical ingredients for quality control of Ligustrum lucidum fruit, were highly correlated with the inhibition of xanthine oxidase activity.

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Section: Plsr Analysis Resultsmentioning

confidence: 97%

Section: Plsr Analysis Resultsmentioning

confidence: 99%

Section: F I G U R E 3 Cluster Analysis Of the Ligustrum Lucidum Extrmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Study on the spectrum‐effect relationship of the xanthine oxidase inhibitory activity of Ligustrum lucidum

Gao

Chen

Zhou

2019

J of Separation Science

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“…When the chromophore was limited, the detection was superior to conventional HPLC 16 . MELC has been successfully applied to determine drugs in pharmaceutical dosage form 17,18 , to analyze biological samples 19,20 , and to separate and determine content in Chinese traditional medicine 21,22 .…”

Section: Introductionmentioning

confidence: 99%

A microemulsion high-performance liquid chromatography (MELC) method for the separation and determination of hydrolyzed tenuifolin in Radix Polygalae

Yan

Zheng

et al. 2019

Sci Rep

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Tenuifolin was used as a reliable chemical marker for the quality control of Radix Polygalae. The determination of tenuifolin is challenging because the analyte molecule lacks a suitable chromophore. The aim of this study was to establish a microemulsion high-performance liquid chromatography (MELC) method which is robust and sensitive, and can separate and determine tenuifolin in Radix Polygalae using an oil-in-water (O/W) microemulsion mobile phase. The separations were performed on a C18 (4.6 × 250 mm, 5 μm) column at 25 °C using a flow rate of 1.0 mL/min, and an ultraviolet detection wavelength of 210 nm. The microemulsion mobile phase comprised 2.8% (w/v) sodium dodecyl sulfate (SDS), 7.0% (v/v) n-butanol, 0.8% (v/v) n-octane and 0.1% (v/v) aqueous orthophosphate buffer (H3PO4). The linearity analysis of tenuifolin showed a correlation coefficient of 0.9923 in the concentration range of 48.00–960.00 µg/mL. The accuracy of the method based on three concentration levels ranged from 96.23% to 99.28%; the limit of detection (LOD) was 2.34 µg/mL, and the limit of quantification (LOQ) was 6.76 µg/mL. The results of our study indicated that the optimized MELC method was sensitive and robust, and can be widely applied for the separation and determination of tenuifolin in Radix Polygalae.

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Drug release evaluation of Paclitaxel/Poly-L-Lactic acid nanoparticles based on a microfluidic chip

Zhang

Guan

Wang

et al. 2021

Biomed Microdevices

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Determination of quantitative retention-activity relationships between pharmacokinetic parameters and biological effectiveness fingerprints of Salvia miltiorrhiza constituents using biopartitioning and microemulsion high-performance liquid chromatography

Cited by 9 publications

References 33 publications

Study on the spectrum‐effect relationship of the xanthine oxidase inhibitory activity of Ligustrum lucidum

Study on the spectrum‐effect relationship of the xanthine oxidase inhibitory activity of Ligustrum lucidum

A microemulsion high-performance liquid chromatography (MELC) method for the separation and determination of hydrolyzed tenuifolin in Radix Polygalae

Drug release evaluation of Paclitaxel/Poly-L-Lactic acid nanoparticles based on a microfluidic chip

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