Determination of Quercetin a Biomarker in Hepatoprotective Polyherbal Formulation through High Performance Thin Layer Chromatography

Gupta, Arti; Sheth, Navin; Sonia, P; ey,; Yadav, Jitendra Singh

doi:10.4172/2157-7064.1000285

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…The interesting fact is that traces of quercetin (12) (m/z 303), a flavonol, were also found in the OsE fraction. Previously quercetin has not reported in O. sanctum although detected in other Ocimum species (Gupta et al 2015;Jain and Mehata 2017).…”

Section: Phytochemical Analysismentioning

confidence: 95%

“…In vivo antioxidant activity of the flavonoids from O. sanctum, was reported as a remarkable reduction in lipid peroxidation and protection of lymphocytes under oxidative stress induced by irradiation (Satyamitra et al 2014;Uma Devi et al 1999). Quercetin in polyherbal preparation including Ocimum is reported to protect liver against oxidative damage (Gupta et al 2015). The extracts of leaves and stem of Ocimum with polyphenols cirsilineol, cirsimaritin, isothymusin, apigenin, rosmarinic acid and eugenol are reported to exhibit antioxidant activity (Mondal et al 2009;Shan et al 2005;Venuprasad et al 2014).…”

Section: Phytochemical Analysismentioning

confidence: 99%

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Phytochemical and antioxidant profiling of Ocimum sanctum

Chaudhary¹,

Sharma²,

Mittal³

et al. 2020

J Food Sci Technol

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The leaves of Ocimum sanctum were extracted in methanol (OsM) and sequentially fractionated with n-hexane (OsH), ethylacetate (OsE) and butanol (OsB) to find the best extraction solvent for antioxidants from the herb known for its medicinal values. OsB was rich in both total polyphenolic content (TPC) (212.26 ± 6.3 mg GAE/ g extract) and total flavonoid contents (TFC) (54.51 ± 3.5 mg QE/g extract). OsE also had significantly high TPC (202.71 ± 5.5 mg GAE/g extract). The EC 50 based on DPPH (3.91 ± 0.3 lg/ml), ABTS (1.6 ± 0.1 lg/ ml) and phosphomolybdate (2.31 ± 0.1 lg/ml) for OsB; hydroxyl (5.3 ± 0.4 lg/ml), superoxide (7.32 ± 0.9 lg/ ml) radicals for OsM and DPPH (8.61 ± 0.6 lg/ml), phosphomolybdate (2.43 ± 0.1 lg/ml) and ABTS (5.3 ± 0.4 lg/ml) for OsE were lower than ascorbic acid showing potential antioxidant properties. EC 50 values of different fractions for DPPH anion, ABTS cation free radical scavenging and phosphomolybdate reducing property were significantly and positively correlated with TPC and TFC. LC-MS analysis of OsB and OsE showed the presence of luteolin, apigenin, rosmarinic, chlorogenic, caffeic acid and their derivatives. Quercetin is extracted in ethylacetate fraction. Overall data revealed that O. sanctum leaf extracts in butanol and ethylacetate with high polyphenolics and flavonoids, had strong antioxidant potential.

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Section: Phytochemical Analysismentioning

confidence: 95%

Section: Phytochemical Analysismentioning

confidence: 99%

Phytochemical and antioxidant profiling of Ocimum sanctum

Chaudhary¹,

Sharma²,

Mittal³

et al. 2020

J Food Sci Technol

View full text Add to dashboard Cite

show abstract

“…Different combinations and ratios of solvents, both for HPLC and HPTLC, have been reported for the detection of various flavonoids in the different types of plant extract [4,5,10,23,24]. Therefore, in this study, the major peaks obtained in the chromatogram of test extract may be of different flavonoids, phytochemically presence of which has been already reported [13,21].…”

Section: Rp-hplc Studymentioning

confidence: 57%

RP-HPLC and HPTLC Identification and Quantification of Flavonoids in the Leaves Extract of Bauhinia acuminata Linn. (Caesalpiniaceae)

Chakraborty¹,

Bala²,

Das

2021

JPRI

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Aims: The present investigation is aimed at the identification and quantification of flavonoids in the methanol extract of leaves of Bauhinia acuminata Linn. (Caesalpiniaceae) by a validated HPTLC method and confirmation of the same by RP-HPLC. Methodology: CAMAG HPTLC system with VisionCATS software and HPLC Agilent Infinity 1260 were employed for the study. Pre-coated silica gel 60 F254 plates were used as stationary phase and Toluene : Ethyl acetate : Formic acid (5:4:0.2, v/v/v) was used as mobile phase in HPTLC while in HPLC Thermo hypersil BDS C18 column and Methanol and 0.4% Phosphoric acid (65 : 35) were used as stationary and mobile phase respectively. Results: Among different standards used in HPTLC, only quercetin was found to be present and further quantitatively analyzed. The method shows good correlation coefficient (R2 ≥ 0.99) and linearity in the range of 0.5 to 5.0 µg/band. The concentration of quercetin in the test extract was found to be 0.514 µg/mg of the extract. The LOD and LOQ values were 0.84 µg/band and 2.59 µg/band respectively. The RP-HPLC study also represented good % RSD of Retention time (0.025) and Area (0.09) which signifies the high precision and repeatability. The assay result (4.99 %) also indicated good presence of the quercetin in the extract. Conclusion: The methods were rapid, cost effective and may be employed for quality-control and standardization of quercetin in different plant extracts.

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Formulation, Evaluation of Physical Properties, and In Vitro Antioxidant Activity Test of Moringa Leaf (Moringa oleifera L.) Ethanolic Extract Capsules

Fatmawati

Sucianingsih

Riswan

et al. 2022

Open Access Maced J Med Sci

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BACKGROUND: Supplements that contain antioxidants may enhance prevention and treatment effects of a wide range of diseases including COVID-19. Quercetin, a flavonoid compound, is a natural antioxidant that can neutralize free radicals. The current study was conducted to formulate Moringa leaf (Moringa oleifera L) ethanol extract capsules and to determine the quercetin antioxidant activity levels of Moringa ethanol extract capsule formulations. MATERIALS AND METHODS: We tested the total flavonoid levels in solutions with concentrations of 20, 50, 60, 70 and 100 ppm using Thin Layer Chromatography Densitometric method. Evaluation of physical properties of 96% Moringa leaf ethanol extract capsules included moisture content test, granule angle of repose test, granule flow property test, capsule weight uniformity test and capsule disintegration time test. Antioxidant activity test using the DPPH method with two samples, namely 96% Moringa leaf ethanol extract capsules with formulas I, II, III, quercetin as a comparison. RESULT: The results of the evaluation of 96% Moringa leaf ethanol extract capsules showed that formula II (PVP 50 mg) had good physical properties. Testing the antioxidant activity of capsules of ethanol extract of Moringa leaves formulas I, II, III, quercetin obtained IC50 values of 44.0 ppm, 40.2 ppm, 46.4 ppm, and 4.80 ppm, respectively. CONCLUSION: The evaluation of the ethanol extract capsules of Moringa leaf formula II, met the parameters of a good capsule evaluation test requirement and had very strong antioxidant activity seen from the acquisition of the IC50 value. The antioxidant properties of Moringa leaf extract capsules may be able to improve the immune system and clinical trials need to be carried out on patients to become candidates for prevention and therapeutic supplement fora range of diseases including COVID-19.

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Determination of Quercetin a Biomarker in Hepatoprotective Polyherbal Formulation through High Performance Thin Layer Chromatography

Cited by 3 publications

References 25 publications

Phytochemical and antioxidant profiling of Ocimum sanctum

Phytochemical and antioxidant profiling of Ocimum sanctum

RP-HPLC and HPTLC Identification and Quantification of Flavonoids in the Leaves Extract of Bauhinia acuminata Linn. (Caesalpiniaceae)

Formulation, Evaluation of Physical Properties, and In Vitro Antioxidant Activity Test of Moringa Leaf (Moringa oleifera L.) Ethanolic Extract Capsules

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