Determination of Quercetin in Extracts and Herbal Products of Phyllanthus niruri by TLC Densitometry Method

Abstract

Phyllanthus niruri has many pharmacological activities such as antioxidant, anti-inflammatory and anticancer. Phyllanthus niruri has been widely developed from extracts into herbal products. The flavonoid quercetin became an analytical marker compound to ensure the quality of P. niruri extracts in the Indonesian herbal pharmacopeia II. This study aimed to determine the levels of quercetin in extract and herbal products using the TLC Densitometry method. TLC Densitometry method using chloroform: ethyl acetate: formic acid (5:4:1) as the mobile phase and silica GF254 as the stationary phase. The Rf value of the quercetin compound obtained is 0.50. The Rs quercetin is 1.56 in the extract and 2.55 in the product. The maximum wavelength for quercetin is 366 nm. The results of quercetin levels were 3.579% in the extract and 0.0412% in herbal products. The TLC densitometry method can analyze quercetin in extracts and herbal products.

Validation of High-Performance Liquid Chromatography (HPLC) Method for Quantification of Ethyl p-Methoxycinnamate in Kaempferia galanga Extract

“…Based on the test results in Table 4, the percentage of EPMC content in the Kaempferia galanga rhizome extract was 54.13%. According to the IHP II, the requirement for EPMC content in Kaempferia galanga rhizome extract is not less than 4.3%, so this study fulfills this requirement 14 . Several previous studies have shown that there is a difference in the percentage of EPMC content in Kaempferia galanga rhizome extract.…”

“…The chromatographic fingerprint is one of the important parameters in the standardization of herbal medicines, which is a method for identifying and determining the content of marker compounds in each plant or herbal medicine to ensure quality and pharmacological activity 12,13 . Marker compounds are compounds or groups of compounds present in plants with or without pharmacological activity 14 . An ideal marker compound should be specific for one plant or available in sufficient quantity for assay.…”