Determination of Redox Status in Different Tissues of Lambs and Kids and Their in-between Relationship

Abstract

The objective of this study was to assess the resting values of the physiological oxidative stress exhibited by lambs and kids reared in Greece, and the potential correlations between redox biomarker levels in blood and other tissues (liver, diaphragm, quadriceps, psoas major muscle). For this purpose, lambs and kids at different developmental stages (d.s.) were used. The latter corresponded to four live weight categories (LWC), each representing 25%, 35%, 70% and 100% of mature body weight. In each of the above tissues, the levels of five common redox biomarkers were determined: glutathione (GSH), catalase (CAT), total antioxidant capacity (TAC), thiobarbituric reactive substances (TBARS), and protein carbonyls (CARBS). The results revealed that lambs and kids belonging to the 35% LWC had weaker endogenous antioxidant pools, while animals in the 70% and 100% LWC had elevated intrinsic antioxidant defense systems. Blood redox biomarkers were associated with the respective ones measured in the diaphragm, liver, quadriceps, and psoas major of both species. Importantly, TBARS levels in blood of animals in the 25% and 100% LWC are correlated with the TBARS levels in all other tissues tested. Blood antioxidant parameters might be used as potential biomarkers to predict the antioxidant status of tissues that affect meat quality. The latter would facilitate quality assessment prior to slaughter, allowing for timely nutritional interventions that can improve meat products.

Evaluation of the effects of a honey‑based gel on blood redox biomarkers and the physiological profile of healthy adults: A pilot study

“…Subsequently, 5 µl of 30% H 2 O 2 were added in the cuvette that was placed in the spectrophotometer (Hitachi, UV-1900) and the optical density was measured twice, at time point 0 and 120 sec, at 240 nm. The calculation of catalase activity was based on the molar extinction coefficient of H 2 O 2 (43.6 M -1 cm -1 ) as previously reported ( 54 ).…”

“…The determination of protein carbonyls was performed according to a study by Skaperda et al ( 54 ). In centrifuge tubes, 50 µl of plasma and 50 µl of 20% (w/v) TCA were added, incubated for 15 min at RT and centrifugated at 15,000 x g for 5 min at 4˚C.…”

“…Following centrifugation of the samples (15,000 x g, for 5 min, at 4˚C), the optical density was monitored at 375 nm (Hitachi, UV-1900). The calculation of the protein carbonyl levels was based on the molar extinction coefficient of DNPH (22x10 3 M -1 cm -1 ) as previously reported ( 54 ).…”

“…Catalase activity. Catalase activity was estimated according to a method described in a study by Aebi (53), which was slightly revised (54). A 1:10 dilution of RBCL (4 µl) was added to 2,996 ml of 67 mmol/l sodium potassium phosphate (pH 7.4) and incubated for 10 min at 37˚C.…”

From Challenge to Opportunity: Addressing Oxidative Stress in Animal Husbandry

“…Polyphenols hold significant promise as feed additives for pigs; they may mitigate the oxidative stress and intestinal toxicity induced by DON [ 8 ]. Interestingly, recent research underscored the significance of redox biomarkers in assessing meat quality in lambs and kids [ 9 ]. By using these biomarkers to make advanced predictions of these issues, timely nutritional interventions can be carried out to ensure optimal meat quality and overall livestock health.…”