Determination of Reduced Nicotinamide Adenine Dinucleotide with a Protamine Multiwalled Carbon Nanotube Electrode

Abstract

A bionanocomposite prepared from multiwalled carbon nanotubes and protamine sulfate is reported for the electrocatalytic determination of dihydronicotinamide adenine dinucleotide.Protamine sulfate was adsorbed via π-π interactions and hydrogen bonds between protonated amines on arginine and surface groups on the multiwalled carbon nanotubes. The multiwalled carbon nanotube protamine composite accumulated dihydronicotinamide adenine dinucleotide on Downloaded by [University of Nebraska, Lincoln] at 12:49 03 October 2015 3 the electrode surface, resulting in high sensitivity and a low limit of detection for dihydronicotinamide adenine dinucleotide. The nanocomposite was employed for dehydrogenase-based biosensing. Alcohol dehydrogenase was allowed to adsorb on the bionanocomposite surface and the sensitivity of the resulting electrode to ethanol was measured.The alcohol biosensor had a excellent sensitivity of 738.5 µA/mM with a low limit of detection of 50 nM.