Determination of residual poly diallyldimethylammonium chloride (pDADMAC) in monoclonal antibody formulations by size exclusion chromatography and evaporative light scattering detector

Khodadadian, Mehdi; Ghassemi, Maryam; Behrouz, Hossein; Maleknia, Shayan; Mahboudi, Fereidoun

doi:10.1016/j.biologicals.2018.11.002

Cited by 2 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The free bacteria are negatively charged due to the presence of lipopolysaccharide in its outer membrane (layer 1). The coating of positively charged PDADMAC leads to positive surface zeta potentials (layers 2 and 4). ,, The encapsulation of negatively charged C-dots, AuNPs, or PAA reverse the surface zeta potentials to negative again (layers 3 and 5). In layer 3 of sample B@P, PAA acts as an anionic polymer conferring a high zeta potential value, which may be due to the carboxylate groups that are present .…”

Section: Results and Discussionmentioning

confidence: 99%

Coencapsulation of Carbon Dots and Gold Nanoparticles over Escherichia coli for Bacterium Assay by Surface-Enhanced Raman Scattering

Anchustegui

Zhu

et al. 2020

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Carbon dots (C-dots) is a type of fluorescent nanomaterials with excellent properties, which in combination with gold nanoparticles (AuNPs) generate structures that can be applied in various fields of science. This research developed a hybrid nanostructure between C-dots and AuNPs using a layer-by-layer (LbL) assembly to increase the surface-enhanced Raman scattering signals (SERS) for the detection of Escherichia coli. The created nanostructure was denoted as B@C-dots@AuNPs and proved by zeta potential, scanning electron microscopy, and fluorescence assays. The results showed that the introduction of AuNPs greatly enhanced the SERS signals of C-dots. The relative intensities of peaks at 2873 and 3082 cm–1 could be used for the detection of bacterium concentration with a detection limit of ca. 102 cfu/mL. In conclusion, an easy, quick, low-cost, and nontoxic technique for the detection of bacteria was developed through the coencapsulation of C-dots and AuNPs over bacteria. The findings are envisaged to facilitate the application of SERS in microbiology and related fields.

show abstract