Determination of Rosmarinic Acid by High-Performance Liquid Chromatography and Its Application to Certain Salvia Species and Rosemary

Abstract

This study describes a method development for the determination of rosmarinic acid (RA) by using a gradient high-performance liquid chromatography (HPLC) and its application to certain plant materials. The analysis was performed by utilizing a two solvents system [A: methanol/water/formic acid (10:88:2; v:v:v); B: methanol/ water/formic acid (90:8:2; v:v:v)] on a reverse-phase column. The flow rate and injection volume were 1 ml min −1 and 10 μl, respectively. Signals were detected at 280 nm. In addition, an internal standard (IS) technique was applied for the analysis of RA to increase precision, and propylparaben was employed for this purpose. The repeatability results as RSD% were 1.66, 1.17 and 1.26 for intra-day and 1.38 was for inter-day with the employment of (3.67×10 −5 M) RA. A limit of linearity (LOL) was observed in a wide (1.13×10 −5 -5.65×10 −4 M) concentration range. Linearity parameters were also examined in the range of 5.95×10 −6 -7.14×10 −5 M RA, and very good correlation was observed. The limit of detection (LOD) and limit of quantification (LOQ) (for inter-day) were 1.60× 10 −6 M (signal/noise [S/N]=3.3) and 4.80×10 −6 M (S/N= 10), respectively. The method was applied to the extracts of certain Lamiaceae plants (Salvia candidissima Vahl. subsp. candidissima, S. sclarea L., S. verticillata L. subsp. verticillata and R. officinalis L.), and reasonable results were obtained.

Bioactivity, biocompatibility and phytochemical assessment of lilac sage, Salvia verticillata L. (Lamiaceae) - A plant rich in rosmarinic acid

“…In very few previous studies addressing the phytochemical characterization of S. verticillata extracts, caffeic acid and its derivatives were the main identified compounds (Fig. 3), with rosmarinic acid being the most abundant bioactive metabolite (Öztürk et al, 2011;Šulniūtė et al, 2017;Tepe et al, 2007), according to our results. Caffeic acid has an essential role in the biosynthesis of secondary metabolites of 2.…”

Validation of Rosmarinic Acid Quantification using High- Performance Liquid Chromatography in Various Plants

“…Recently, rosmarinic acid was detected using several mobile phase systems of isocratic and gradient HPLC. The HPLC system used acetonitrile-water and methanol-water in combination with phosphoric acid showed the best separation of rosmarinic acid 6,14 . However, rosmarinic acid detection takes a lengthy period due to its long retention time.…”

“…As the caffeic acid ester of 3,4-dihydroxyphenyllactic acid, rosmarinic acid is a phenylpropanoid. 6,7 .…”

“…The results show that the efficacy is due in part to the presence of phenolic acids, specifically rosmarinic acid, which has been found in large amounts in a number of therapeutic plants. 6 .…”

“…On the previous study, rosmarinic acid was determined using gradient HPLC method, LC/ MS/MS, spectrophotometry method and Fourier transform infrared 3,6,14 . HPLC is a simple and reliable methods for routine analysis and easily used for quantification of individual compounds.…”