Determination of rubella virus‐specific cell‐mediated immunity using IFNγ‐ELISpot

Allmendinger, J.; Paradies, F.; Kamprad, Manja; Richter, Tina; Pustowoit, Barbara; Liebert, Uwe G.

doi:10.1002/jmv.21621

Cited by 7 publications

(3 citation statements)

References 35 publications

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“…Similarly, high IL-10 production has been inversely correlated with influenza antibody production following trivalent influenza vaccine vaccination (6). Contrary to these findings, our group and others have demonstrated minimal or no correlation between cellular immune responses and the antibody response to measles and rubella viruses following MMR vaccination (1,7,28).…”

contrasting

confidence: 69%

Correlations Between Vaccinia-Specific Immune Responses Within a Cohort of Armed Forces Members

et al. 2011

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Widespread vaccination with vaccinia virus (VACV) resulted in the eradication of smallpox; however, the licensed VACV-containing vaccines are associated with adverse events (AEs), making them unsuitable for certain high-risk populations. A better understanding of the host immune response following smallpox vaccination could result in vaccines with similar immunogenicity profiles to pre-eradication vaccines with a lower incidence of AEs. To study the immune response to VACV, we recruited 1,076 armed forces members who had been vaccinated with one dose of Dryvax Ò . We measured multiple VACV-specific immune responses: neutralizing antibody titer, the level of 12 secreted cytokines in peripheral blood mononuclear cell (PBMC) cultures (IL-1b, IL-2, IL-4, IL-6, IL-10, IL-12p40, IL-12p70, TNF-a, IFN-c, IFN-a, , and the number of IFNc-and CD8 + IFN-c-secreting cells. We analyzed these data to determine correlations between immune response measures. We detected a strong proinflammatory response in concert with a Th-1-like cytokine response pattern at a median time point of 15.3 mo following primary vaccination. We also detected correlations between neutralizing antibody titer and secreted IL-2, as well as secreted IFN-c ( p = 0.009 and p = 0.0007, respectively). We also detected strong correlations between the proinflammatory cytokines IL-1b, TNF-a, IL-6, and IL-12p40 ( p < 0.0001). These results further advance our knowledge of vaccinia-specific cellular immune responses. Notably, vaccine-induced proinflammatory responses were not correlated with neutralizing antibody titers, suggesting that further attenuation to reduce inflammatory immune responses may result in decreased AEs without sacrificing VACV immunogenicity and population seropositivity.

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confidence: 69%

Correlations Between Vaccinia-Specific Immune Responses Within a Cohort of Armed Forces Members

et al. 2011

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“…However, there have been few reports regarding the combination of humoral and cellular immunity against rubella [11,[13][14][15][16]. The present study focused on the antibody changes associated with cellular immunity over a two-year interval following vaccination.…”

Section: Discussionmentioning

confidence: 99%

Changes of Antibody Associated with Cell-Mediated Immunity in College Students During Two Years after Rubella Vaccination

Terada¹,

Hagihara²,

Tanaka³

et al. 2017

J Vaccines Vaccin

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Objective: This study measured cell-mediated immunity (CMI) and serum antibody levels against rubella to clarify antibody changes associated with CMI during 2 years after vaccination. Methods:The study subjects were 64 college students in two groups: 38 subjects who (having exhibited hemagglutination-inhibition (HI) anti-rubella antibody titers of ≤ 1:16) had been vaccinated with a rubella vaccine 2 years previously (Group 1); and 26 subjects who (having exhibited HI titers ≥ 1:32) had not been vaccinated 2 years previously (Group 2). All students were tested for IgG antibody levels (by enzyme-linked immunosorbent assay) and CMI (by interferon-γ (IFN-γ release assay). Results:The IgG antibody and IFN-γ values decreased significantly in Group 1 during the 2 years postvaccination, while antibody titers in Group 2 did not decrease over this interval. The proportion of Group-1 individuals with HI antibody titers ≤ 1:16 increased to 27/38 (71.1%) at two years following vaccination, compared with 6/26 (23.1%) in Group 2. No correlation of IgG antibody titers was observed in Group 1 between one month and two years after vaccination. However, a strong correlation (r=0.85, p<0.00001) was detected in Group 2 between two years ago and the present. The correlation of IFN-γ values in Group 1 between one month and two years after vaccination was 0.614 (p<0.00005). When the subjects were classified by CMI status, the numbers of individuals who were intermediate and negative for IgG antibody at two years after vaccination were 1 and 0 of 13 subjects, 3 and 1 of 9 subjects, and 3 and 2 of 16 subjects in Group 1, respectively, while the antibody-positive status was maintained in all subjects in Group 2. Conclusion:The IgG antibody and IFN-γ values decreased in distinct ways during the 2 years of the study; the persistence of the seropositivity was associated with CMI status.

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“…However both are particularly essential for molecular epidemiologic surveillance, they have some limitations in clinical diagnosis due to the degradation of virus and its RNA as a result of incorrect transport or storage conditions also the restricted time for sample collection as a result of short duration of viral shedding [Organization ; Abernathy et al, , Wandinger et al, ]. For these reasons recently acquired rubella is commonly confirmed by RV‐specific IgM antibody detection in the serum [Abernathy et al, , Allmendinger et al, ]. However, rubella primary infection is not always the only cause of IgM positivity [Wilson et al, ].…”

Section: Introductionmentioning

confidence: 99%

The etiology of Rubella IgM positivity in patients with rubella‐like illness in Iran from 2011 to 2013

Khorrami

Mokhtari‐Azad

Yavarian

et al. 2015

Journal of Medical Virology

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Rubella is a mild self-limiting contagious viral disease caused by the rubella virus (RV). Although symptoms are often mild, the concern is centralized around the possible effect on a fetus growth and development in case of primary infection during early months of pregnancy. Recently acquired rubella is commonly confirmed by RV-specific IgM antibody detection in the serum. However, rubella primary infection is not always the only cause of IgM positivity. Other possible causes of rubella IgM positivity may include IgM persistence following vaccination or naturally acquired infection or even re-infection. Moreover, nonspecific IgM reactivity can cause false-positive results. There are few articles to differentiate the aetiology of rash in rubella-like illnesses. However, limited studies have been conducted on clarifying the source of IgM positivity in these cases. This article reports the study of 10,896 clinical cases demonstrating rubella-like illness between 2011 and 2013 in Iran. The rate of IgM positivity among these cases was 0.52% (57 cases). As predicted based on the high coverage of vaccination in Iran fewer than 16% of cases with ELISA IgM positive result, were due to current rubella primary infections. The greater part of the positive IgM reactions occurred in cross reactivity with other viruses (31.6%) or in prolonged IgM response post vaccination (24.6%). This research confirmed that the positive result of rubella IgM assay in vaccinated individuals is mainly caused by prolonged IgM production, rubella re-infection, and false positivity due to infection with other viruses, rather than the rubella primary infection itself.

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Determination of rubella virus‐specific cell‐mediated immunity using IFNγ‐ELISpot

Cited by 7 publications

References 35 publications

Correlations Between Vaccinia-Specific Immune Responses Within a Cohort of Armed Forces Members

Correlations Between Vaccinia-Specific Immune Responses Within a Cohort of Armed Forces Members

Changes of Antibody Associated with Cell-Mediated Immunity in College Students During Two Years after Rubella Vaccination

The etiology of Rubella IgM positivity in patients with rubella‐like illness in Iran from 2011 to 2013

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