Determination of SARS‐coronavirus by a microfluidic chip system

Zhou, Xiaomian; Liu, Dayu; Zhong, Runtao; Dai, Zhongpeng; Wu, Dapeng; Wang, Hui; Du, Yifeng; Xia, Zian; Zhang, Liping; Mei, Xiaodai; Lin, Baiquan

doi:10.1002/elps.200305966

Cited by 104 publications

(83 citation statements)

References 14 publications

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“…Holes were punched out of the PDMS slab to form reservoirs for introduction of liquid. The bottom layer was fabricated by wet etching method as described previously [21,22], which included 20 microcolumns. Each microcolumn is 5 mm high, 150 mm wide and 1 mm long.…”

Section: Microfluidic Device Design and Fabricationmentioning

confidence: 99%

Characterization of the interaction between fibroblasts and tumor cells on a microfluidic co‐culture device

Liu

Qin

et al. 2010

Electrophoresis

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Fibroblasts and tumor cells have been involved in the process of cancer development, progression and therapy. Here, we present a simple microfluidic device which enables to study the interaction between fibroblasts and tumor cells by indirect contact co-culture. The device is composed of multiple cell culture chambers which are connected by a parallel of cell migration regions, and it enables to realize different types of cells to communicate each other on the single device. In this work, human embryonic lung fibroblasts cells were observed to exhibit obvious migration towards tumor cells instead of normal epithelial cells on the co-culture device. Moreover, transdifferentiation of human embryonic lung fibroblast cells was recognized by the specific expression of a-smooth musle actin, indicating the effect of tumor cells on the behavior of fibroblasts. Furthermore, multiple types of cell co-culture can be demonstrated on the single device which enables to mimic the complicated microenviroment in vivo. The device is simple and easy to operate, which enables to realize real-time observation of cell migration after external stimulus. This microfluidic device allows for the characterization of various cellular events on a single device sequentially, faciliating the better understanding of interaction between heterotypic cells in a more complex microenvironment. Keywords:Co-culture / Microfluidic / Migration / Transdifferentiation DOI 10.1002/elps.200900776 IntroductionGrowing evidences demonstrate that tumor microenvironment including stromal cells, inflammatory cells, extracellular matrix and vessels plays an important role in the development, progression and therapy of cancer [1][2][3][4][5].Fibroblasts make up the largest number of stromal cells, which are continuously influenced by soluble factors secreted by tumor cells and their migration abilities can be enhanced by tumor cells. When fibroblasts are transdifferentiated, they become activated and were termed cancerassociated fibroblasts or myofibroblasts, which will have a significant effect on the proliferation, invasion and metastasis of tumor cells [6][7][8]. Therefore, it is of great importance to investigate the heterotypic cell interactions between tumor cells and fibroblasts. At present, co-culture is often used to investigate the interactions between heterotypic cells by using Transwell assay [9,10], in which two types of cells are separated into upper and lower chambers by a semi-permeable membrane, or by bathing cells in conditioned medium [11][12][13][14]. However, these assays are limited by the tedious manual operations which require several procedures, and they are all typical end-point assays, which are difficult to realize real-time observation. In addition, they still require large consumption of cells and reagents that are expensive in amount.Microfluidics has sparked increasing interests in cellbased biological and medical analysis, due to its miniaturization, low consumption of reagents, and capabilities to integrate various experimental op...

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Section: Microfluidic Device Design and Fabricationmentioning

confidence: 99%

Characterization of the interaction between fibroblasts and tumor cells on a microfluidic co‐culture device

Liu

Qin

et al. 2010

Electrophoresis

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“…For example, chip electrophoresis of viral RNA, or DNA produced via PCR, served for genotyping Hepatitis B [1] and Hepatitis C virus [2], for the detection of Dengue-2 virus [3], of the Coronavirus causing severe acute respiratory syndrome (SARS) [4], for analysis of DNA samples of Cytomegalovirus [5] and Herpes simplex virus [6]. Only in a few cases, the viruses themselves were subjected to electrophoresis; e.g.…”

Section: Introductionmentioning

confidence: 99%

Electrophoresis on a microfluidic chip for analysis of fluorescence‐labeled human rhinovirus

et al. 2007

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We report the analysis of human rhinovirus serotype 2 (HRV2) on a commercially available lab-on-a-chip instrument. Due to lack of sufficient native fluorescence, the proteinaceous capsid of HRV2 was labeled with Cy5 for detection by the red laser (lambda ex 630 nm) implemented in the instrument. On the microdevice, electrophoresis of the labeled virus was possible in a BGE without stabilizing detergents, which is in contrast to conventional CE; moreover, analysis times were drastically shortened to the few 10 s range. Resolution of the sample constituents (virions, a contaminant present in all virus preparations, and excess dye) was improved upon adaptation of the separation conditions, mainly by adjusting the SDS concentration of the BGE. Purity of fractions from size-exclusion chromatography after labeling of virus was assessed, and affinity complex formation of the labeled virus with various recombinant very-low-density lipoprotein receptor derivatives differing in the number of concatenated V3 ligand binding repeats was monitored. Virus analysis on microchip devices is of particular interest for experiments with infectious material because of easy containment and disposal of samples. Thus, the employment of microchip devices in routine analysis of viruses appears to be exceptionally attractive.

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“…Glass microchips were fabricated using standard photolithography and wet chemical etching technique as described elsewhere [25]. The photography of the microchip and a schematic of the enlarged partial channel structure are shown in Fig.…”

Section: Microchips and Instrumentsmentioning

confidence: 99%

A multichannel electrophoresis microchip platform for rapid chiral selector screening

et al. 2008

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This study presents a four-channel electrophoresis chip platform, featuring double-cross hydrostatic sample injection, for rapid chiral selector screening. This platform needs only five electrodes to drive microchip electrophoresis in four separate channels for screening four chiral selectors at a time. To demonstrate the performance of this screening platform, eight neutral CDs and their derivatives as chiral selectors were screened towards two FITC-labeled chiral compounds. The screening could be accomplished in less than 2 min. Dimethyl-beta-CD and hydroxypropyl-alpha-CD was demonstrated to be the appropriate selectors for FITC-norfenefrine and FITC-baclofen, respectively. The established platform is easy to operate and suitable for rapid screening process, which is expected to be a potential platform for high-throughput screening of chiral selectors.

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Determination of SARS‐coronavirus by a microfluidic chip system

Cited by 104 publications

References 14 publications

Characterization of the interaction between fibroblasts and tumor cells on a microfluidic co‐culture device

Characterization of the interaction between fibroblasts and tumor cells on a microfluidic co‐culture device

Electrophoresis on a microfluidic chip for analysis of fluorescence‐labeled human rhinovirus

A multichannel electrophoresis microchip platform for rapid chiral selector screening

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