Determination of selenium concentration and glutathione peroxidase activity in plasma and erythrocytes.

Pleban, Patricia A.; Munyani, A; Beachum, J

doi:10.1093/clinchem/28.2.311

Cited by 183 publications

(60 citation statements)

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“…Varying results have been obtained regarding the correlation in man between the erythrocyte GSH-Px activity and the selenium content in plasma and erythrocytes (25,26). These discrepancies might be explained by the low proportion of bIood selenium associated with GSH-Px in man (10-15%) (26,27), as compared, for example, with sheep (75%) ( 2 8 ) .…”

Section: Discussionmentioning

confidence: 99%

Increased glutathione peroxidase activity in erythrocytes in patients with Alzheimer's disease/senile dementia of Alzheimer's type

Annerén

Gardner

Lundin

1986

Acta Neurologica Scandinavica

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Glutathione peroxidase (GSH-Px) activity in erythrocytes was studied in 9 patients with Alzheimer's disease/senile dementia of Alzheimer's type (AD/SDAT) of ages 34-64 years and compared with that in 16 healthy controls of ages 21-66 years. The median GSH-Px activity in erythrocytes from AD/SDAT patients was 408, mu kat/1 (range 338-500 mu kat/1) and that from healthy controls 348, mu kat/1 (range 258-439 mu kat). This difference was significant (p less than 0.005) Wilcoxon rank test. Since there are great clinical and pathological similarities between AD/SDAT and the dementia of Down's syndrome (DS) patients and since both these groups of patients have increased activity of the selenium-dependent enzyme GSH-Px, it is suggested that there could be a similar metabolic background of the dementia. Many findings in the oxidative metabolism of DS suggest increased oxidative damage with an elevation of the turnover of superoxides to peroxides within cells and with a secondary biochemical modification such as increase in tissual GSH-Px activity. A similar mechanism with elevated peroxidation within cells might be responsible for the present finding of increased GSH-Px activity in erythrocytes and for the development of dementia in AD/SDAT.

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Section: Discussionmentioning

confidence: 99%

Increased glutathione peroxidase activity in erythrocytes in patients with Alzheimer's disease/senile dementia of Alzheimer's type

Annerén

Gardner

Lundin

1986

Acta Neurologica Scandinavica

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“…Glutathione peroxidase was determined by spectrophotometric determination of the nicotinamide adenine dinucleotide phosphate (NADPH) consumption rate in the presence of hydrogen peroxide at 340 nm according to previously described methods. 25 The ORAC assay was conducted with an automated plate reader k and slightly modified from that described by Huang et al 26 The ORAC assay determines the ability of a sample to protect fluorescein from a thermally controlled radical generator, 2,2 0 -azobis (2-amidino-propane) dihydrochloride at 371C. The kinetics of the reaction were monitored for 70 minutes in a thermally controlled plate reader and the fluorescence (ex 485, em 520) was determined every 2 minutes.…”

Section: Methodsmentioning

confidence: 99%

Serial determination of biomarkers of oxidative stress and antioxidant status in dogs with naturally occurring gastric dilatation‐volvulus

Walker

Chan

Freeman

et al. 2007

J Vet Emergen Crit Care

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Objective: To serially determine the biomarkers of oxidative stress and antioxidant status of dogs with gastric dilatation‐volvulus (GDV) over 48 hours. Design: Prospective study. Setting: Veterinary teaching hospital intensive care unit. Animals: Thirteen client‐owned dogs with GDV. Interventions: None. Measurement and main results: For all dogs, biomarkers of oxidative stress and antioxidant status were ascertained. Indicators of oxidative stress measured included F2α‐isoprostanes (isoprostanes) and malondialdehyde (MDA). Vitamin C, vitamin E, glutathione peroxidase (GSHPx), and oxygen radical absorbance capacity (ORAC) were measured to assess antioxidant status. Oxidants and antioxidants were measured at the time of admission and at 24 and 48 hours post‐admission. There were significant decreases in vitamin E (P=0.002), vitamin C (P=0.001), ORAC (P=0.02), and MDA (P=0.001) during hospitalization. There was no significant change in GSHPx and isoprostane concentrations over time. Conclusion: Oxidative stress and antioxidant capacity in dogs with GDV change over time. Further studies measuring oxidative stress in more severely affected dogs and performing measurements earlier in the disease process may be beneficial in determining when oxidative stress is most severe during the course of GDV management.

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“…The activity of CuZn-SOD in erythrocyte lysate was measured using the method described by Fitzgerald et al (15). The activity of GSH-Px in erythrocyte lysate was measured using the method described by Pleban et al (16) and the level of MDA in plasma was determined by the method described by Richard et al (17). All oxidative damage parameters were measured at the onset of the study (SOD 1, GSH-Px 1, MDA 1) and at remission (SOD 2, GSH-Px 2, MDA 2) within a period ranging from 6 to 17 wk.…”

Section: Methodsmentioning

confidence: 99%

Effect of vitamin E treatment on the oxidative damage occurring in Henoch‐Schönlein purpura

Erdoğan¹,

Öner²,

Aydın³

et al. 2003

Acta Paediatrica

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Aim: To evaluate the role of reactive oxygen molecules (ROMs) in the pathogenesis of Henoch‐Schonlein purpura and the effect of vitamin E on oxidative damage. ROMs have been suggested to contribute in many pathological conditions including renal diseases and vasculitis. Methods: The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH‐Px) as antioxidant enzymes were measured, and the level of malondialdehyde (MDA) as an indicator of lipid peroxidation in 27 children with Henoch‐Schönlein purpura at the onset of the disease and during the remission period. The results of this study were compared with those of 11 healthy children studied as a control group. Results: With regard to all the oxidative damage parameters such as SOD, GSH‐Px and MDA, significant differences were detected between the patients and the control group in both the acute and remission periods. But no such differences were detected between patients with and those without renal involvement. In 15 patients receiving vitamin E treatment, oxidative damage parameters and clinical course showed no improvement despite significant increases in plasma vitamin E levels. Conclusion: Oxidative damage and lipid peroxidation may play an important part in the pathogenesis of Henoch‐Schonlein purpura but vitamin E given after initiation of lipid peroxidation, which is the last phase of cellular damage, is of no use in breaking down the oxidative chain reactions that have already been triggered.

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Determination of selenium concentration and glutathione peroxidase activity in plasma and erythrocytes.

Cited by 183 publications

References 0 publications

Increased glutathione peroxidase activity in erythrocytes in patients with Alzheimer's disease/senile dementia of Alzheimer's type

Increased glutathione peroxidase activity in erythrocytes in patients with Alzheimer's disease/senile dementia of Alzheimer's type

Serial determination of biomarkers of oxidative stress and antioxidant status in dogs with naturally occurring gastric dilatation‐volvulus

Effect of vitamin E treatment on the oxidative damage occurring in Henoch‐Schönlein purpura

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