Determination of selenium species in seawater by flow injection hydride generation in situ trapping followed by electrothermal atomic absorption spectrometry†

Cabon, Jean-Yves; Erler, Wolfgang

doi:10.1039/a802541j

Cited by 46 publications

(18 citation statements)

References 36 publications

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“…Selenite was measured by hydride generation atomic absorption spectrometry (HGAAS) involving reduction of Se (IV) to hydrogen selenide (H 2 Se) using hydrochloric acid (HCl) combined with sodium borohydride (NaBH 4 ) [28,29]. The HGAAS system consisted of an AAnalyst 800™ Atomic Absorption Spectrometer (PerkinElmer, USA) with an MHA-FIAS-400 flow injector and an AS-90 plus autosampler.…”

Section: Assay Of Semet and Selenitementioning

confidence: 99%

Biotransformation of l-Selenomethionine and Selenite in Rat Gut Contents

Krittaphol

McDowell

Thomson

et al. 2010

Biol Trace Elem Res

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L-selenomethionine (SeMet) and sodium selenite are widely used selenium nutritional supplements with potential benefit in preventing cancer. However, supplementation is not without risks of toxicity if intake is too high. The aim of the present study was to investigate SeMet and selenite metabolism in the gastrointestinal tract with particular focus on the formation of the volatile selenium excretion products, dimethylselenide (DMSe) and dimethyldiselenide (DMDSe). Adult male Wistar rats (n = 5) were euthanized, their intestinal tracts removed and the contents of jejunum, ileum, caecum and colon used to prepare 10% suspensions in saline. SeMet and selenite (0.5-0.6 mM) were then incubated with these suspensions at 37°C for 3 h. Caecum and colon contents were the most metabolically active towards SeMet with 30% and 15% metabolized over 3 h. DMDSe was the only volatile selenium metabolite detected accounting for 8.7 ± 1.3% of the selenium lost in caecum contents. Selenite was completely metabolized by caecum contents and 73% by colon contents under the same conditions forming DMSe (5.7 ± 0.9% of the selenium lost in caecum) and a precipitate of red amorphous elemental selenium. Based on previous literature and these results, we conclude that the gut microbiota contributes to the excretion of excess selenium through the production of methylated selenium compounds and elemental selenium.

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Section: Assay Of Semet and Selenitementioning

confidence: 99%

Biotransformation of l-Selenomethionine and Selenite in Rat Gut Contents

Krittaphol

McDowell

Thomson

et al. 2010

Biol Trace Elem Res

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“…A similar level of selenium content in marine water samples with di®erent speciation (0.20 ng/ml Se(VI) and 0.10 ng/ml Se(IV)) was reported Cabon [12] and by Kubota (0.19 ng/ml of total selenium concentration) [23]. Higher concentrations of selenium { up to a few ng/ml { were obtained by Larraya [24], who reported a higher Unauthenticated Download Date | 8/28/18 10:35 PM concentration of Se(IV) (1.8 ng/ml), while the concentration of Se(VI) compounds was 0.98 ng/ml.…”

Section: Determination Of Selenium In Marine Water Samplesmentioning

confidence: 70%

“…In order to enhance the e®ectiveness of the pre-concentration, the walls of the graphite tube are covered with a modi¯er, which is either introduced at the appropriate stage of the thermal cycle or electrothermally deposited [3]. The modi¯ers can be the compounds of palladium [4][5][6][7][8], magnesium [6,9,10], zirconium [11], iridium [8,[11][12][13][14], or permanent modi¯ers like Zr, Ir, W, Nb [5,15,16]. Palladium and other metals are able to bind selenium as intermetallic compounds, which evaporate at temperatures higher than selenium itself [17,18], although this mechanism has been criticised by other authors [19].…”

Section: Introductionmentioning

confidence: 99%

Hydride generation atomic absorption spectrometry with insitu graphite tube trapping for the determination of Se (IV) and Se (VI) in baltic sea water samples

2003

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This paper reports the results of an optimisation study for a procedure to determine the total selenium and its inorganic species, Se(IV) and Se(VI) using atomic absorption spectrometry combined with hydride generation and in-situ trapping of the analyte on the inner walls of the graphite tube. With the use of the proposed modi cation, a detection limit (3¼ ) of 0.018 ng/ml is achieved. This paper presents exemplary results, according to the proposed procedure, for selenium determination in samples of marine water. The concentrations of selenium in the samples ranged from < 0.02 ng/ml to 0.16 ng/ml of Se(IV) and from < 0.02 ng/ml to 0.10 ng/ml of Se(VI).

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“…Selenite was analyzed by hydride generation atomic absorption spectrometry (HGAAS) [17,18] and DMSe and DMDSe by gas chromatography-mass spectrometry [8,19] as described elsewhere [4]. Serum selenium was determined by graphite furnace atomic absorption spectrometry based on a modification of the method of Jacobson and Lockitch [20].…”

Section: Methodsmentioning

confidence: 99%

Metabolism of l-Selenomethionine and Selenite by Probiotic Bacteria: In Vitro and In Vivo Studies

Krittaphol

Wescombe²,

Thomson

et al. 2011

Biol Trace Elem Res

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Since selenium supplements have been shown to undergo biotransformation in the gut, probiotic treatment in combination with selenium supplements may change selenium disposition. We investigated the metabolism of L-selenomethionine (SeMet) and selenite by probiotic bacteria in vitro and the disposition of selenium after probiotic treatment followed by oral dosing with SeMet and selenite in rats. When SeMet was incubated anaerobically with individual antibiotic-resistant probiotic strains (Streptococcus salivarius K12, Lactobacillus rhamnosus 67B, Lactobacillus acidophilus L10, and Bifidobacterium lactis LAFTI® B94) at 37°C for 24 h, 11-18% was metabolized with 44-80% of SeMet lost being converted to dimethyldiselenide (DMDSe) and dimethylselenide (DMSe). In similar incubations with selenite, metabolism was more extensive (26-100%) particularly by the lactobacilli with 0-4.8% of selenite lost being converted to DMSe and DMDSe accompanied by the formation of elemental selenium. Four groups of rats (n = 5/group) received a single oral dose of either SeMet or selenite (2 mg selenium/kg) at the time of the last dose of a probiotic mixture or its vehicle (lyoprotectant mixture used to maintain cell viability) administered every 12 h for 3 days. Another three groups of rats (n = 3/group) received a single oral dose of saline or SeMet or selenite at the same dose (untreated rats). Serum selenium concentrations over the subsequent 24 h were not significantly different between probiotic and vehicle treated rats but appeared to be more sustained (SeMet) or higher (selenite) than in the corresponding groups of untreated rats. Probiotic treated rats given SeMet also had selenium concentrations at 24 h that were significantly higher in liver and lower in kidney than untreated rats given SeMet. Thus, treatment with probiotics followed by SeMet significantly affects tissue levels of selenium.

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Determination of selenium species in seawater by flow injection hydride generation in situ trapping followed by electrothermal atomic absorption spectrometry†

Cited by 46 publications

References 36 publications

Biotransformation of l-Selenomethionine and Selenite in Rat Gut Contents

Biotransformation of l-Selenomethionine and Selenite in Rat Gut Contents

Hydride generation atomic absorption spectrometry with insitu graphite tube trapping for the determination of Se (IV) and Se (VI) in baltic sea water samples

Metabolism of l-Selenomethionine and Selenite by Probiotic Bacteria: In Vitro and In Vivo Studies

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