Determination of Selenomethionine by High-Performance Liquid Chromatography–Fluorescence Detection Coupled with On-line Oxidation

Rapid determination of amino acids in biological samples using a monolithic silica column

“…As shown in Table 2, the limits of detection (LODs) for individual NBD-amino acids ranged from 3.2 to 57.2 fmol at a signal-to-noise ratio of 3, and the limits of quantification (LOQs) ranged from 10.8 to 191 fmol at a signal-to-noise ratio of 10. These values of LOD and LOQ for each NBD-amino acid were comparable to those obtained in our previous studies as well as in other studies (Aoyama et al 2004;Aoyama et al 2009;Song et al 2011). The precision and accuracy data for the determination of the NBD-amino acids are listed in Table 3.…”

Amino acids analysis using a monolithic silica column after derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F)

“…NBD-F has several merits such as a fast and efficient reaction with primary and secondary amino acids and a long wavelength of excitation and fluorescence, which leads to a relatively high sensitivity [14][15][16][17][18]. However, the analysis is very long and takes about 80 min, which made routine analysis inconvenient, especially in the field of clinical therapy.…”

Recent advances in development and application of derivatization reagents having a benzofurazan structure: a brief overview

“…NBD–amino acid derivatives were separated on a core–shell C 18 column within 8 min and detected with high sensitivity (Song et al ., ), and NBD‐labeled branched‐chain amino acids were separated on a pillar array column (Song et al ., ). NBD‐F was also used for the analysis of selenomethionine (Aoyama et al ., ), fluoxetine, norfluoxetine (Higashi et al ., ), amantadine (Zhang et al ., ), gabapentine (Yagi et al ., ) and bisoprolol (Ulu and Aydogmus, ). Eto et al .…”

“…NBD-amino acid derivatives were separated on a core-shell C 18 column within 8 min and detected with high sensitivity (Song et al, 2013a), and NBD-labeled branched-chain amino acids were separated on a pillar array column (Song et al, 2013b). NBD-F was also used for the analysis of selenomethionine (Aoyama et al, 2009), fluoxetine, norfluoxetine , amantadine (Zhang et al, 2011), gabapentine (Yagi et al, 2012) and bisoprolol (Ulu and Aydogmus, 2012). Eto et al (2011) used NBD-F for the analysis of D,L-amino acids in food samples, where the NBD-amino acids were separated on a reversed-phase column and detected by circular dichroism at 235 nm.…”