Determination of serum aluminum, platelet aggregation and lipid peroxidation in hemodialyzed patients

Abstract

Aluminum (Al 3+ ) overload is frequently associated with lipid peroxidation and neurological disorders. Aluminum accumulation is also reported to be related to renal impairment, anemia and other clinical complications in hemodialysis patients. The aim of the present study was to determine the degree of lipid peroxidation, platelet aggregation and serum aluminum in patients receiving regular hemodialytic treatment. The level of plasma lipid peroxidation was evaluated on the basis of thiobarbituric acid reactive substances (TBARS). Mean platelet peroxidation in patients undergoing hemodialysis was significantly higher than in normal controls (2.7 ± 0.03 vs 1.8 ± 0.06 nmol/l, P<0.05). Platelet aggregation and serum aluminum levels were determined by a turbidimetric method and atomic absorption spectrophotometry, respectively. Serum aluminum was significantly higher in patients than in normal controls (44.5 ± 29 vs 10.8 ± 2.5 µg/l, P<0.05). Human blood platelets were stimulated with collagen (2.2 µg/ml), adenosine diphosphate (6 µM) and epinephrine (6 µM) and showed reduced function with the three agonists utilized. No correlation between aluminum levels and platelet aggregation or between aluminum and peroxidation was observed in hemodialyzed patients.

Elevated levels of erythrocyte-conjugated dienes indicate increased lipid peroxidation in schistosomiasis mansoni patients

“…These markers correlate closely with other methods commonly used to detect lipid peroxidation and are wellcharacterized and reliable indicators (7,13). However, while the TBARS assay is frequently used as a measure of MDA, with data expressed as nmol MDA per liter plasma (12,14) as we did in the present study, the assay is not specific for MDA since other compounds including sugars, amino acids and bilirubin may cross-react with thiobarbituric acid. Thus, in future studies it may be preferable to use HPLC, a more precise though complex analysis (14).…”

“…Young patients (11)(12)(13)(14)(15)(16)(17)(18)(19)(20) years; N = 18) of both genders with hepatosplenic schistosomiasis mansoni, who had had upper digestive bleeding but no renal, cardiac, hepatitis, or other parasite/microbial associated disease were included in this study. They were outpatients at the Clinical Hospital, Federal University of Pernambuco (UFPE), Recife, and had been treated with the antischistosomal drug, oxamniquine (a single dose of 20 mg/ kg) followed by splenectomy, ligature of the left gastric vein and auto-implantation of spleen tissue into an omental pouch of the major omentum.…”

“…Samples were cooled to room temperature, centrifuged at 3,000 g for 5 min and their absorbance was measured at 535 nm against a reaction mixture lacking plasma but subjected to the same procedure. As in other studies (12), levels of TBARS are reported as nmol of MDA per liter of plasma, using a standard curve prepared with MDA bis (diethyl acetal) as the MDA reference source. Conjugated dienes in washed erythrocytes were extracted with dichloromethane/methanol (2:1, v/v) and measured by the method of Buege and Aust (11).…”

Evaluation of Toxic Metals in Blood and Urine Samples of Chronic Renal Failure Patients, before and after Dialysis

“…However, the protective function of the intestinal barrier is less effective in the end stage of renal disease patients than healthy individuals. [38] Due to poverty and unawareness, poor people used alum for water purification. People who have a normal urological system may be not affected significantly, but in those with renal disorders, the toxicity due to Al can become severe.…”

Effect of pathogenic yeasts on human platelet aggregation

“…Measurement of PA. PA was determined by the turbidimetric method [19] using a Net Lab aggregometer. Aliquots of 450 µL of a platelet suspension were transferred into small cuvettes and stirred at a constant speed of 180g at 37ºC.…”