Elevated levels of erythrocyte-conjugated dienes indicate increased lipid peroxidation in schistosomiasis mansoni patients

Abstract

Schistosoma mansoni causes liver disease by inducing granulomatous inflammation. This favors formation of reactive oxygen species, including superoxide ions, hydrogen peroxide and hydroxyl radicals all of which may induce lipid peroxidation. We have evaluated lipid peroxidation in 18 patients with hepatosplenic schistosomiasis mansoni previously treated with oxamniquine followed by splenectomy, ligature of the left gastric vein and auto-implantation of spleen tissue, by measuring levels of erythrocyte-conjugated dienes and plasma malondialdehyde (MDA). Age-matched, healthy individuals (N = 18) formed the control group. Erythrocyteconjugated dienes were extracted with dichloromethane/methanol and quantified by UV spectrophotometry, while plasma MDA was measured by reaction with thiobarbituric acid. Patient erythrocytes contained two times more conjugated dienes than control cells (584.5 ± 67.8 vs 271.7 ± 20.1 µmol/l, P < 0.001), whereas the increase in plasma MDA concentration (about 10%) was not statistically significant. These elevated conjugated dienes in patients infected by S. mansoni suggest increased lipid peroxidation in cell membranes, although this was not evident when a common marker of oxidative stress, plasma MDA, was measured. Nevertheless, these two markers of lipid peroxidation, circulating MDA and erythrocyte-conjugated dienes, correlated significantly in both patient (r = 0.62; P < 0.01) and control (r = 0.57; P < 0.05) groups. Our data show that patients with schistosomiasis have abnormal lipid peroxidation, with elevated erythrocyte-conjugated dienes implying dysfunctional cell membranes, and also imply that this may be attenuated by the redox capacity of antioxidant agents, which prevent accumulation of plasma MDA. Correspondence

Malondialdehyde; Lipid peroxidation plasma biomarker correlated with hepatic fibrosis in human Schistosoma mansoni infection

“…In accord with earlier findings of (Facundo et al 2004), who reported 10% increase of plasma indicator MDA concentration in 18 patients with mansoni schistosomiasis, treated surgically by splenectomy, when compared to control group, but statistically couldn't show significant rise. This may be explained due to limited number of cases.…”

Levels of oxidative stress markers: correlation with hepatic function and worm burden patients with schistosomiasis

“…Regarding the significant increase in the TBARS/MDA in the test group, it was observed that these data were also reported in other papers, which show an association between the Schistosoma mansoni and high levels of reactive substances to thiobarbituric acid (TBARS) and reactive oxygen species (ROS) (Facundo et al 2004;Del Rio et al 2005;Eboumbou et al 2005). In man, infection with S. mansoni is associated with increased levels of circulating TBARS and ROS adducts (Pascal et al 2000).…”

Approaches and Methods to Measure Oxidative Stress in Clinical Samples: Research Applications in the Cancer Field

“…Unsaturated hydroxyalkenal 4-HNE can be investigated in tissues, preferably using immunohistochemistry (IHC), or HPLC [82, 83]. CDs, produced as a consequence of free radical-induced autoxidation of polyunsaturated fatty acids (PUFAs), can be detected according to Suryanarayana Rao and Recknagel, which follows the maximal absorption of UV light at 233 nm by these compounds [84–86]. Finally, LOOHs, which are the primary oxidation products of PUFAs, can be determined by the ferrous oxidation xylenol orange (FOX) assay, based on the ability of LOOH to oxidize ferrous iron in the presence of xylenol orange, leading to the formation of a colored ferric-xylenol orange complex, with an absorbance at 560 nm [87, 88].…”