Determination of serum triglyceride by enzyme electrode using covalently immobilized enzyme on egg shell membrane

Narang, Jagriti; Minakshi,; Bhambi, Manu; Pundir, C.S.

doi:10.1016/j.ijbiomac.2010.09.001

Cited by 31 publications

(7 citation statements)

References 15 publications

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“…Glycerol reacts with the glycerol-3-kinase (GK) and gets converted to to glycerol-3-phosphate which then serves as the substrate for glycerol-3-phosphate oxidase to form dihydroxyacetonephosphate and H 2 O 2 ( Figure 5B). The redox potential of H 2 O 2 is about 0.4 V. [4,26,31] The other potential contributor to the redox signal is FAD whose redox potential also has been reported between 0.4-0.5 V. [46] We did not observe any redox peak at that potential in the present study. Instead, we have observed a redox peak between 0.2 to 0.3 V which is attributed to the redox potential of FAD.…”

Section: Electrochemical Measurementssupporting

confidence: 48%

“…The glycerol is then converted by glycerol kinase (GK) to glycerol-3-phosphate which is further converted by glycerol-3-phosphate oxidase (GPO) into dihydroxyacetone phosphate and hydrogen peroxide (H 2 O 2 ). [24][25][26] The electrochemical signal can arise either due to the irreversible conversion of H 2 O 2 or the reversible redox reactions involving the coenzymes FAD and FADH 2 . There are several attempts reported in literature to develop disposable, quick, highly selective and sensitive triglyceride biosensors using the three enzyme mix.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of a Nano‐Interfaced Electrochemical Triglyceride Biosensor and its Potential Application towards Distinguishing Cancer and Normal Cells

et al. 2020

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Hypertriglyceridemia, a condition characterized by elevated levels of triglycerides is considered a major risk factor of metabolic syndrome. The present work aims to develop a hybrid nano-interfaced enzymatic sensor that employs three enzymes to hydrolyze the triglycerides, phosphorylate glycerol and finally oxidize it to generate the electrochemical signal. Triolein, a common long chained triglyceride found in biological systems was employed as the substrate. The sensor displayed an excellent response time of 3 s and a linear range of 0.5 to 5.5 mM. The sensor was stable under both wet and dry conditions for 25 days. The capability of the sensor to detect triglycerides in biological samples was assessed by employing it to estimate total triglyceride levels in normal and cancer cells. Cancer cells displayed triglyceride levels distinctly higher than normal cells suggesting that this sensor can be employed for screening normal and cancer tissues apart from routine serum analysis to screen individuals with triglyceridemia and metabolic syndrome.

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Section: Electrochemical Measurementssupporting

confidence: 48%

Section: Introductionmentioning

confidence: 99%

Fabrication of a Nano‐Interfaced Electrochemical Triglyceride Biosensor and its Potential Application towards Distinguishing Cancer and Normal Cells

et al. 2020

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“…Amperometric TG biosensors employing different membranes such as polyvinyl acetate (PVA) [17], gelatin membrane [28], egg shell [29], cellulose acetate (CA) [16] and PVC membranes [15,30] have been fabricated in recent years. Various techniques such as physical adsorption, covalent binding, microencapsulation, entrapment, cross-linking were used to immobilize enzymes on these membranes.…”

Section: Membrane Based Tg Biosensorsmentioning

confidence: 99%

Recent trends and perspectives in enzyme based biosensor development for the screening of triglycerides: a comprehensive review

Hooda

Gahlaut

Gothwal

2018

Artificial Cells, Nanomedicine, and Biotechnology

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Clinical manifestations of the elevated plasma triacylglycerol (TG) include a greater prevalence of atherosclerotic heart disease, acute pancreatitis, diabetes mellitus, hypertension, and ischemic vascular disease. Hence, these significant health troubles have attracted scientific attention for the precise detection of TG in biological samples. Numerous techniques have been employed to quantify TG over many decades, but biosensors hold the leading position owing to their superior traits such as highly specific recognition for target molecules, accuracy, minituarization, small sample requirement and rapid response. Enzyme-based electrochemical biosensors represent an instantaneous resolution for the foremost bottlenecks constraining laboratory prototypes to reach real time bedside applications. We highlight the choice of transducers and constructive strategies to design high-performance biosensor for the quantification of triglycerides in sera and early diagnosis of health problems related to it. In the present review, a small effort has been made to emphasize the significant role of enzymes, nanostructured metal oxides, graphene, conducting polypyrrole, nanoparticles, porous silicon, EISCAP and ENFET in enabling TG biosensors more proficient and taking a revolutionary step forward.

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“…Therefore, biosensors offer advantages for triglyceride determination due to relatively low cost, ease of use and good selectivity. The most recent reports of triglyceride biosensors found in literature [1][2][3] are based on interactions of three enzymes, lipase, glycerol kinase (GK) and glycerol-3-phosphate oxidase (GPO). Pundir & Narang [4] reported an electrochemical sensor for triglyceride by measuring the oxygen consumption or the amount of hydrogen peroxide produced by the enzymatic reactions.…”

Section: Introductionmentioning

confidence: 99%

The Electroanalytical Detection of Triglyceride Concentrations in Olive Oil using Modified Screen Printed Electrodes with Ionic Liquid [1-(2-Ethoxyethyl)-1-Methylpyrrolidinium Bis(Trifluoromethylsulfonyl)Imide]-Lipase

Zain¹

2017

IJBSBE

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The electroanalytical detection of triglyceride concentrations in olive oil using modified screen printed electrodes with Ionic Liquid [1-(2-ethoxyethyl)-1-methylpyrrolidiniumbis (trifluoromethylsulfonyl)imide]-lipase was reported for the first time. In this study, 1-(2-ethoxy-ethyl)-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide, [C 2 OC 2 C 1 pyrr][N(Tf) 2 ] was used as an ionic liquid due to the high ionic conductivity and good biocompatibility to enhance the electrochemical response. The mixture of [C 2 OC 2 C 1 pyrr][N(Tf) 2 ]-GA-Lipase was immobilized on the surface of the screen printed carbon electrodes (SPCE) by drop casting technique. The electrochemical performance and mechanism of C/ [C 2 OC 2 C 1 pyrr][N(Tf) 2 ]-GA-Lipase modified electrode was investigated using cyclic voltammetry (CV). The surface morphology of the modified SPCE was also studied using SEM. The optimization parameters of C/[C 2 OC 2 C 1 pyrr][N(Tf) 2 ]-GA-Lipase electrodes were carried out, and shows that pH 7, 30°C and 5 % of lipase enzyme loading resulted in optimum performance. The electroanalytical methodology proposed in this manuscript was successfully applied in the determination of triglyceride concentrations in olive oil. The limit of detection (LOD) and response time were 0.68 mM and 46 seconds respectively. A linear calibration range from 0.67 to 2.68 mM was also obtained. Common interfering compounds on the sensor were investigated using cyclic voltammetry. Furthermore, the biosensor was effectively validated using gas chromatography analysis.

show abstract

Determination of serum triglyceride by enzyme electrode using covalently immobilized enzyme on egg shell membrane

Cited by 31 publications

References 15 publications

Fabrication of a Nano‐Interfaced Electrochemical Triglyceride Biosensor and its Potential Application towards Distinguishing Cancer and Normal Cells

Fabrication of a Nano‐Interfaced Electrochemical Triglyceride Biosensor and its Potential Application towards Distinguishing Cancer and Normal Cells

Recent trends and perspectives in enzyme based biosensor development for the screening of triglycerides: a comprehensive review

The Electroanalytical Detection of Triglyceride Concentrations in Olive Oil using Modified Screen Printed Electrodes with Ionic Liquid [1-(2-Ethoxyethyl)-1-Methylpyrrolidinium Bis(Trifluoromethylsulfonyl)Imide]-Lipase

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