Determination of serum triglycerides by an accurate enzymatic method not affected by free glycerol.

Sullivan, David; Kruijswijk, Z.; West, C.E.; Kohlmeier, MacLean G.; Katan, Martijn B.

doi:10.1093/clinchem/31.7.1227

Cited by 143 publications

(36 citation statements)

References 1 publication

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“…The glycogen content was estimated according to the anthrone-sulfuric acid method (Zwaan & Zandee, 1972) with a commercially available kit (N. A043, Jiancheng Bioengineering Institute, Nanjing, China). The triglyceride content was assessed using the modified glycerol-3-phosphate oxidase-p-aminophenazone method (Sullivan, Kruijswijk, West, Kohlmeier, & Katan, 1985) with a commercially available kit (N. A110-1, Jiancheng Bioengineering Institute, Nanjing, China). The total protein content was determined according to the method described by Schaffner and Weissmann (1973) using a commercially available kit (N. A045-4, Jiancheng Bioengineering Institute, Nanjing, China).…”

Section: Gonadal Histology and Stages Of Gonadal Developmentmentioning

confidence: 99%

Comparative studies of the growth, survival, and reproduction of diploid and triploid Kumamoto oyster, Crassostrea sikamea

Zhang

Xiao

et al. 2019

J World Aquaculture Soc

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Triploid oysters have been used for farming to improve growth but have not been created in the Kumamoto oyster, Crassostrea sikamea, which is one of the crucial aquaculture species on the southern coast of China. In the present study, triploids were created using cytochalasin B to inhibit polar body II release in C. sikamea, with the untreated oysters as controls. Triploidy rates of 87 and 57.67%, on average, were obtained in larvae and adults, respectively. Larval growth and survival of the triploid were significantly lower than that of the controls (p < 0.05). In contrast, the triploid postlarvae and adults had a significant growth advantage over the controls (p < 0.05) during the period of 180 (December) to 450 days (September of the next year). Moreover, the triploids clearly exhibited significant sterility in the reproductive season. The glycogen and triglyceride contents in the gonad, adductor muscle, mantle, and gill were higher in triploids than in controls from 180 to 450 days. As a result, high physiological energy supply was strongly correlated with superior growth and reduced reproduction in triploid C. sikamea. The triploid C. sikamea is an excellent oyster species and can be used to improve growth for C. sikamea farming.

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Section: Gonadal Histology and Stages Of Gonadal Developmentmentioning

confidence: 99%

Comparative studies of the growth, survival, and reproduction of diploid and triploid Kumamoto oyster, Crassostrea sikamea

Zhang

Xiao

et al. 2019

J World Aquaculture Soc

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“…The levels of serum total cholesterol (TC), triglycerides (TG), and high-and low-density lipoprotein cholesterols (HDL-C & LDL-C), were analyzed using Analyticon Biotechnologies kit, Germany, according to the techniques designated by Allain, Poon, Chan, Richmond, and Fu (1974), Nauck et al (1997), Sullivan, Kruijswijk, West, Kohlmeier, and Katan (1985), and Wieland and Seidel (1983), respectively. The levels of very low-lipoprotein triglycerides (VLDL-TG) were computed according to the Friedewald formula: VLDL-TG = TG/5.…”

Section: Biochemical Analysis Determination Of Serum Lipid Profilementioning

confidence: 99%

Ameliorative role of a combination of chitosan, calcium, vitamins A and E against high fat diets-induced adverse effects in rats

Ali¹,

Mansour²,

Hassanine³

et al. 2019

JoBAZ

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Background: Chitosan is a promising natural source for controlling obesity. Obese people tend to use supplementary nutrients to compensate for their deficiencies in their diets. The present study aimed to investigate the combined effect of chitosan, calcium, and vitamins A and E supplements against the adverse effects of high-fat diet consumption in rats. Methods: Twenty-five male albino rats were assigned into five equal groups. Control rats were fed standard basal diet (SBD). Another group was fed high-fat diet (HFD) without supplements. The other three groups were fed HFD containing 0, 400, and 800 mg of chitosan per kilogram diet, in presence of calcium and vitamins A and E supplements, daily for 10 weeks. Results: As compared to controls, rats fed HFD without supplements showed significant elevations in body weight gain, feed consumption, relative weights of the heart and liver, serum levels of total cholesterol, triglycerides, low-density lipoprotein cholesterols, very low-lipoprotein triglycerides, urea, creatinine, and malondialdehyde, as well as serum activities of alanine and aspartate aminotransferases, alkaline phosphatase, and creatine kinase. Moreover, significant declines in the relative kidney weight, serum levels of high-density lipoprotein cholesterols, total proteins, albumin, globulin, calcium, vitamins A and E, erythrocyte glutathione content, and superoxide dismutase activity were recorded. Histopathological alterations were observed in rats fed HFD with or without supplements. On contrary, rats fed HFD containing chitosan and supplements showed remarkable improvement in all the studied parameters towards control values, in dose-dependent manner. Conclusion: Chitosan, calcium, and vitamins A and E attenuated the adverse effects caused by HFD intake in rats.

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“…Serum was collected into clean sample bottles for biochemical assays. Lipid profile was evaluated by assessing Total cholesterol (Tc) [Siedel et al, 1983], Triacylglycerides (TAG) [Sullivan et al, 1985], High Density Lipoprotein (HDLc), and Low Density Lipoprotein (LDL-c) [Friedewald et al, 1992]. All analysis was performed using commercially available kits based on the references and Manufacturer's instructions using analyzer.…”

Section: Estimation Of Lipid Profilementioning

confidence: 99%

Methanol Extract of Unfermented Theobroma Cacao Promotes Normal Lipid Profile of Wistar Rats

Agwupuye¹,

Beshel²,

Ezeanyika³

2019

IJTSRD

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Abnormal lipid metabolism is a major pathogenic factor for various cardiovascular diseases. The present study investigated the lipid profile of methanol extract of unfermented Theobroma cacao (TC) in Wistar rats. 24 Male Wistar rats were divided equally into four groups. Group 1 was the control group, and was administered 0.9% normal saline. Groups 2, 3 and 4 were administered 200mg/kg, 400mg/kg and 800mg/kg methanol extract of unfermented TC. Administration was via oral gavage and lasted for 21 days. The rats were sacrificed under chloroform anaesthesia. Blood was collected through cardiac puncture, allowed to clot, and later centrifuged to get serum. Laboratory assays were done for serum concentrations of total cholesterol (Tc), triacylglycerides (TAG), high density lipoprotein (HDL-c), and low density lipoprotein (LDL-c). Administration of TC extract resulted in an increase (p<0.05) serum concentration of HDL-c, with a consequent reduction in the serum concentrations of Tc, TAG, and LDL-c when compared with the control. The observed results showed that consumption of unfermented Theobroma cacao within the experimental dose promotes normal lipid profile of Wistar rats. Thus, if these results are extrapolated to man, consumption of unfermented Theobroma cacao is encouraged.

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Determination of serum triglycerides by an accurate enzymatic method not affected by free glycerol.

Cited by 143 publications

References 1 publication

Comparative studies of the growth, survival, and reproduction of diploid and triploid Kumamoto oyster, Crassostrea sikamea

Comparative studies of the growth, survival, and reproduction of diploid and triploid Kumamoto oyster, Crassostrea sikamea

Ameliorative role of a combination of chitosan, calcium, vitamins A and E against high fat diets-induced adverse effects in rats

Methanol Extract of Unfermented Theobroma Cacao Promotes Normal Lipid Profile of Wistar Rats

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