Determination of sinigrin, sinalbin, allyl- and benzyl isothiocyanates by RP-HPLC in mustard powder extracts

Herzallah, S. M.; Holley, Richard A.

doi:10.1016/j.lwt.2012.01.022

Cited by 43 publications

(28 citation statements)

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“…Samples were maintained at either 100 or 75 °C for 5, 10, 30 and 60 min and intact glucosinolate concentrations analysed with HPLC following the adapted Herzallah and Holly method [19]. …”

Section: Methodsmentioning

confidence: 99%

Development of an efficient glucosinolate extraction method

et al. 2017

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BackgroundGlucosinolates, anionic sulfur rich secondary metabolites, have been extensively studied because of their occurrence in the agriculturally important brassicaceae and their impact on human and animal health. There is also increasing interest in the biofumigant properties of toxic glucosinolate hydrolysis products as a method to control agricultural pests. Evaluating biofumigation potential requires rapid and accurate quantification of glucosinolates, but current commonly used methods of extraction prior to analysis involve a number of time consuming and hazardous steps; this study aimed to develop an improved method for glucosinolate extraction.ResultsThree methods previously used to extract glucosinolates from brassicaceae tissues, namely extraction in cold methanol, extraction in boiling methanol, and extraction in boiling water were compared across tissue type (root, stem leaf) and four brassicaceae species (B. juncea, S. alba, R. sativus, and E. sativa). Cold methanol extraction was shown to perform as well or better than all other tested methods for extraction of glucosinolates with the exception of glucoraphasatin in R. sativus shoots. It was also demonstrated that lyophilisation methods, routinely used during extraction to allow tissue disruption, can reduce final glucosinolate concentrations and that extracting from frozen wet tissue samples in cold 80% methanol is more effective.ConclusionsWe present a simplified method for extracting glucosinolates from plant tissues which does not require the use of a freeze drier or boiling methanol, and is therefore less hazardous, and more time and cost effective. The presented method has been shown to have comparable or improved glucosinolate extraction efficiency relative to the commonly used ISO method for major glucosinolates in the Brassicaceae species studied: sinigrin and gluconasturtiin in B. juncea; sinalbin, glucotropaeolin, and gluconasturtiin in S. alba; glucoraphenin and glucoraphasatin in R. sativus; and glucosatavin, glucoerucin and glucoraphanin in E. sativa.Electronic supplementary materialThe online version of this article (doi:10.1186/s13007-017-0164-8) contains supplementary material, which is available to authorized users.

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“…Samples were maintained at either 100 or 75 °C for 5, 10, 30 and 60 min and intact glucosinolate concentrations analysed with HPLC following the adapted Herzallah and Holly method [19]. …”

Section: Methodsmentioning

confidence: 99%

Development of an efficient glucosinolate extraction method

et al. 2017

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“…The yellow mustard seeds provided the characteristic ions that corresponded to sinalbin. The content of sinalbin in the yellow mustard seeds was quantified by the DART‐TOF‐MS and verified by HPLC/UV; the results from both methods were similar and corresponded to the values reported in the literature . The DART‐TOF‐MS method can be used for qualitative and semi‐quantitative analyses of glucosinolates in mustard seeds.…”

Section: Resultsmentioning

confidence: 99%

Characterization of mustard seeds and paste by DART ionization with time‐of‐flight mass spectrometry

et al. 2014

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Direct analysis in real time (DART) is a novel technique with great potential for rapid screening analysis. The DART ionization method coupled with high-resolution time-of-flight mass spectrometry (TOF-MS) has been used for characterization of mustard seeds and table mustard. The possibility to use DART to analyse glucosinolates was confirmed on determination of sinalbin (4-hydroxybenzyl glucosinolate). The DART-TOF-MS method was optimized and validated. A set of samples of mustard seeds and mustard products was analyzed. High-performance liquid chromatography and DART-TOF-MS were used to determine glucosinolates in mustard seeds and compared. The correlation equation between these methods was DART = 0.797*HPLC + 6.987, R(2) = 0.972. The DART technique seems to be a suitable method for evaluation of the quality of mustard seeds and mustard products.

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“…Isocratic elution was carried out for 10 min at a flow rate of 1 ml/min, using a solvent system containing 20% (v/v) acetonitrile and 80% water containing 0.02 M pH 5.5 tetrabutylammonium hydrogen sulfate (TBA) as an ion-pair agent. The injection volume used was 5 μl and the detector absorbance was set at 227 nm (Herzallah and Holley, 2012;Tsao et al, 2002).…”

Section: Verification Of the Absence Or Presence Of Active Myrosinasementioning

confidence: 99%

Contribution of endogenous plant myrosinase to the antimicrobial activity of deodorized mustard against Escherichia coli O157:H7 in fermented dry sausage

Cordeiro

Holley

2014

International Journal of Food Microbiology

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Determination of sinigrin, sinalbin, allyl- and benzyl isothiocyanates by RP-HPLC in mustard powder extracts

Cited by 43 publications

References 50 publications

Development of an efficient glucosinolate extraction method

Development of an efficient glucosinolate extraction method

Characterization of mustard seeds and paste by DART ionization with time‐of‐flight mass spectrometry

Contribution of endogenous plant myrosinase to the antimicrobial activity of deodorized mustard against Escherichia coli O157:H7 in fermented dry sausage

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